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Recombinant Mouse LIF Protein

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D3 mouse ESCs were cultured with Recombinant Mouse LIF (10 ng/mL). After 15 days, and following multiple rounds of passaging, the ESCs were analyzed for pluripotency by staining with PE-conjugated Mouse Anti-Human/Mouse SSEA‑1 PE-conjugated Monoclonal Antibody (Catalog # FAB2155P) and APC-conjugated Mouse Anti-Human/Mouse SSEA‑4 APC-conjugated Monoclonal Antibody (Catalog # FAB1435A). ESCs continue to express the pluripotency marker SSEA-1 and lack expression of SSEA-4, a negative marker for mouse ESCs. Quadrants were set based on samples stained with isotype control antibodies.
D3 mouse ESCs were cultured with Recombinant Mouse LIF (10 ng/mL). After 15 days, and following multiple rounds of passaging, the ESCs were analyzed for pluripotency by staining with PE-conjugated Mouse ...read more
D3 mouse ESCs were cultured in Recombinant Mouse LIF (10 ng/mL) for 15 days, including 4 passages. ESCs continue to express the mouse pluripotency markers, SSEA-1 (red) and SOX2 (green). SSEA-1 was visualized with the ...read more
Recombinant Mouse LIF(Catalog # 8878-LF) induces IL-6 secretion in M1 mouse myeloid leukemia cells. The ED50 for for this effect is 0.1-0.6ng/mL

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
8878-LF-025/CF
Carrier Free
8878-LF-100/CF
Carrier Free
8878-LF-500/CF
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Recombinant Mouse LIF Protein Summary

Details of Functionality
Measured by its ability to induce IL-6 secretion by M1 mouse myeloid leukemia cells. The ED50 for this effect is 0.1-0.6 ng/mL.
Source
E. coli-derived mouse LIF protein
Pro25-Phe203
Accession #
N-terminal Sequence
Pro25
Protein/Peptide Type
Recombinant Proteins
Gene
Lif
Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
20 kDa, reducing conditions
Publications
Read Publications using
8878-LF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 0.2-1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse LIF Protein

  • CDF
  • D Factor
  • DIA
  • differentiation inhibitory activity
  • differentiation stimulating factor
  • Differentiation-stimulating factor
  • Emfilermin
  • HILDA
  • HILDAcholinergic differentiation factor
  • leukemia inhibitory factor (cholinergic differentiation factor)
  • leukemia inhibitory factor
  • LIF
  • Melanoma-derived LPL inhibitor
  • MLPLI

Background

Recombinant mouse LIF (leukemia inhibitory factor) is commonly used in cell culture to maintain the pluripotency of stem cells. LIF is a widely expressed pleiotropic member of the IL-6 family of cytokines (1-3). Mature mouse LIF is expressed as a highly and variably glycosylated 32-62 kDa monomer that shares 78%, 91%, 80%, 76%, and 78% aa sequence identity with human, rat, canine, bovine, and porcine LIF, respectively (4). LIF functions through a heterodimeric receptor complex containing a ligand-binding subunit, LIF R alpha /CD118, and a signal transducing subunit, gp130 (2, 4, 5). gp130 also serves as a subunit of the receptor complexes for Oncostatin M, Cardiotrophin-1, CNTF, IL-6, IL-11, and IL-27 (2, 5). A soluble form of mouse LIF R alpha can be generated by alternative splicing (6). Depending on the cells and their context, LIF either opposes or favors differentiation (2, 7). LIF produced by the uterine endometrium supports successful implantation of the embryo, promotes proliferation and maintenance of pluripotency in embryonic stem cells, and favors proliferation of progenitor cell types such as hematopoietic stem cells (2, 5, 7). However, excess LIF blocks differentiation of embryoid bodies, indicating the importance of LIF regulation (2, 5). LIF is produced by activated CD4+ T cells and is required by the thymic epithelium to support T cell maturation (2, 3). Its expression is upregulated by neuronal injury, and it promotes motor neuron survival and oligodendrocyte myelination (2, 3, 8). It is produced by the adrenal cortex and likely enhances adrenal production of cortisol and aldosterone (9). LIF can also function as an autocrine growth factor in some pancreatic cancers, but it induces differentiation in the myeloid leukemic cell line M1 (1, 10). Tumor cell-derived LIF can also induce formation of immunosuppressive tumor-associated macrophages (11). LIF promotes endometrial remodeling and differentiation of adipocytes and cardiac smooth muscle cells (2, 3, 12). It promotes regulatory T cell and inhibits Th17 cell differentiation, thus down-regulating inflammation and contributing to immune tolerance during pregnancy and in the nervous system (2, 3, 5, 7).
  1. Moreau, J.F. et al. (1988) Nature 336:690.
  2. Trouillas, M. et al. (2009) Eur. Cytokine Netw. 20:51.
  3. Metcalfe, S.M. (2011) Genes Immun. 12:157.
  4. Gearing, D.P. et al. (1987) EMBO J. 6:3995.
  5. Cheng, J.G. et al. (2001) Proc. Natl. Acad. Sci. USA 98:8680.
  6. Tomida, M. et al. (1993) FEBS lett. 334:193.
  7. Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319.
  8. Slaets, H. et al. (2010) Trends Mol. Med. 16:493.
  9. Bamberger, A.M. et al. (2000) Mol. Cell. Endocrinol. 162:145.
  10. Kamohara, H. et al. (2007) Int. J. Oncol. 30:977.
  11. Duluc, D. et al. (2007) Blood 110:4319.
  12. Zouein, F.A. et al. (2013) Eur. Cytokine Netw. 24:11.

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Publications for LIF (8878-LF)(15)

We have publications tested in 2 confirmed species: Mouse, Transgenic Mouse.

We have publications tested in 2 applications: Bioassay, In vivo assay.


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Bioassay
(14)
In vivo assay
(1)
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Mouse
(14)
Transgenic Mouse
(1)
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using 8878-LF Applications Species
Gogoi, M;Clark, PA;Ferreira, ACF;Rodriguez Rodriguez, N;Heycock, M;Ko, M;Murphy, JE;Chen, V;Luan, SL;Jolin, HE;McKenzie, ANJ; ILC2-derived LIF licences progress from tissue to systemic immunity Nature 2024-08-07 [PMID: 39112698] (In vivo assay, Transgenic Mouse) In vivo assay Transgenic Mouse
Donahue, KL;Watkoske, HR;Kadiyala, P;Du, W;Brown, K;Scales, MK;Elhossiny, AM;Espinoza, CE;Lasse Opsahl, EL;Griffith, BD;Wen, Y;Sun, L;Velez-Delgado, A;Renollet, NM;Morales, J;Nedzesky, NM;Baliira, RK;Menjivar, RE;Medina-Cabrera, PI;Rao, A;Allen, B;Shi, J;Frankel, TL;Carpenter, ES;Bednar, F;Zhang, Y;Pasca di Magliano, M; Oncogenic KRAS-dependent stromal interleukin-33 directs the pancreatic microenvironment to promote tumor growth Cancer discovery 2024-07-03 [PMID: 38958646] (Bioassay, Mouse) Bioassay Mouse
Bilgic, SN;Domaniku, A;Toledo, B;Agca, S;Weber, BZC;Arabaci, DH;Ozornek, Z;Lause, P;Thissen, JP;Loumaye, A;Kir, S; EDA2R-NIK signalling promotes muscle atrophy linked to cancer cachexia Nature 2023-05-01 [PMID: 37165186] (Bioassay, Mouse) Bioassay Mouse
FA Graca, M Rai, LC Hunt, A Stephan, YD Wang, B Gordon, R Wang, G Quarato, B Xu, Y Fan, M Labelle, F Demontis The myokine Fibcd1 is an endogenous determinant of myofiber size and mitigates cancer-induced myofiber atrophy Nature Communications, 2022-05-02;13(1):2370. 2022-05-02 [PMID: 35501350] (Bioassay, Mouse) Bioassay Mouse
I Kelava, I Chiaradia, L Pellegrini, AT Kalinka, MA Lancaster Androgens increase excitatory neurogenic potential in human brain organoids Nature, 2022-01-19;0(0):. 2022-01-19 [PMID: 35046577] (Bioassay, Mouse) Bioassay Mouse
K Noh, H Lee, TY Choi, Y Joo, SJ Kim, H Kim, JY Kim, JW Jahng, S Lee, SY Choi, SJ Lee Negr1 controls adult hippocampal neurogenesis and affective behaviors Mol. Psychiatry, 2019-01-16;0(0):. 2019-01-16 [PMID: 30651602] (Bioassay, Mouse) Bioassay Mouse
Y Tang, S Xiong, P Yu, F Liu, L Cheng Direct Conversion of Mouse Fibroblasts into Neural Stem Cells by Chemical Cocktail Requires Stepwise Activation of Growth Factors and Nup210 Cell Rep, 2018-07-31;24(5):1355-1362.e3. 2018-07-31 [PMID: 30067988] (Bioassay, Mouse) Bioassay Mouse
YY Wang, S Ma, Q Chen, D Jiao, Y Yang In vivo selection with lentiviral expression of Bcl2T69A/S70A/S87Amutant in hematopoietic stem cell-transplanted mice Gene Ther., 2018-03-09;0(0):. 2018-03-09 [PMID: 29523881] (Bioassay, Mouse) Bioassay Mouse
J Du, X Wang, X Zhang, X Zhang, H Jiang DNER modulates the length, polarity and synaptogenesis of spiral ganglion neurons via the Notch signaling pathway Mol Med Rep, 2017-11-20;17(2):2357-2365. 2017-11-20 [PMID: 29207144] (Bioassay, Mouse) Bioassay Mouse
J Yang, C Wu, I Stefanescu, A Horowitz Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies J Vis Exp, 2017-04-22;0(122):. 2017-04-22 [PMID: 28518115] (Bioassay, Mouse) Bioassay Mouse
Show All 15 Publications.

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Bioinformatics

Gene Symbol Lif
Uniprot