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Recombinant Mouse BMP-4 Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Mouse BMP-4 Protein, CF Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. <EM>et al</EM>. (1999) Exp. Cell Res. <STRONG>250</STRONG>:351.<br />The ED<sub>50</sub> for this effect is&nbsp;5-40 ng/mL.<br /><br />
Accession #
N-terminal Sequence
Ser293
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Bmp4
Purity
>95.000%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
Endotoxin Note
<0.010 EU per 1 µg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
13.1 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
18-26 kDa, reducing conditions
Publications
Read Publications using
5020-BP/CF in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>95.000%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
Reconstitution Instructions
Reconstitute at 100 μg/mL in 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse BMP-4 Protein, CF

  • BMP2B
  • BMP-2B
  • BMP2B1
  • BMP2BMCOPS6
  • BMP4
  • BMP-4
  • Bone morphogenetic protein 2B
  • bone morphogenetic protein 4
  • DVR4
  • MCOPS6
  • OFC11
  • ZYME

Background

BMP-4 is a TGF-beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1-5). The mouse BMP-4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). The propeptide is cleaved intracellularly by furin or proprotein convertase 6, enabling the 15 kDa mature BMP-4 monomer to form an active disulfide linked homodimer or heterodimer with BMP-7 (7-9). Mature mouse and human BMP-4 share 98% aa sequence identity. Mouse BMP-4 shares 85% aa sequence identity with mouse BMP-2 and 35%-54% with other mouse BMPs. Compared to BMP-4 homodimers, BMP-4/BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP-4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPR-IA) and type II (primarily Activin RIIA or BMPR-II) receptors (11, 12). The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans (13-15).

  1. Zhang, P. et al. (2008) Blood 111:1933.
  2. Gambaro, K. et al. (2006) Cell Death Differ. 13:1075.
  3. Simic, P. and S. Vukicevic (2005) Cytokine Growth Factor Rev. 16:299.
  4. Sadlon, T.J. et al. (2004) Stem Cells 22:457.
  5. Frank, D.B. et al. (2005) Circ. Res. 97:496.
  6. Oida, S. et al. (1995) DNA Seq. 5:273.
  7. Cui, Y. et al. (1998) EMBO J. 17:4735.
  8. Cui, Y. et al. (2001) Genes Dev. 15:2797.
  9. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
  10. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
  11. Chen, D. et al. (2004) Growth Factors 22:233.
  12. Lavery, K. et al. (2008) J. Biol. Chem. April 24 epub.
  13. Rosen, V. (2006) Ann. N.Y. Acad. Sci. 1068:19.
  14. Jones, C.M. and J.C. Smith (1998) Dev. Biol. 194:12.
  15. Takada, T. et al. (2003) J. Biol. Chem. 278:43229.

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5020-BP/CF
Species: Mu
Applications: Bioactivity

Publications for BMP-4 (5020-BP/CF)(108)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Ptychodera flava, Rabbit, Sea Urchin.

We have publications tested in 5 applications: Bioassay, Cell Culture, ELISA (Capture), In Vivo, Western Blot Control.


Filter By Application
Bioassay
(96)
Cell Culture
(6)
ELISA (Capture)
(1)
In Vivo
(2)
Western Blot Control
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(101)
Rat
(3)
Ptychodera flava
(1)
Rabbit
(1)
Sea Urchin
(2)
All Species
Showing Publications 1 - 10 of 108. Show All 108 Publications.
Publications using 5020-BP/CF Applications Species
M Manuel, KB Tan, Z Kozic, M Molinek, TS Marcos, MFA Razak, D Dobolyi, R Dobie, BEP Henderson, NC Henderson, WK Chan, MI Daw, JO Mason, DJ Price Pax6 limits the competence of developing cerebral cortical cells to respond to inductive intercellular signals PloS Biology, 2022-09-06;20(9):e3001563. 2022-09-06 [PMID: 36067211] (Bioassay, Mouse) Bioassay Mouse
X Chen, L Chen, G Lin, Z Wang, MC Kodali, M Li, H Chen, SG Lebovitz, TC Ortyl, L Li, S Ismael, P Singh, KU Malik, T Ishrat, FM Zhou, W Zheng, FF Liao White matter damage as a consequence of vascular dysfunction in a spontaneous mouse model of chronic mild chronic hypoperfusion with eNOS deficiency Molecular Psychiatry, 2022-08-10;0(0):. 2022-08-10 [PMID: 35948662] (Cell Culture, Mouse) Cell Culture Mouse
T Yamamoto, Z Diao, M Murakami, F Shimokawa, T Matsui, M Funaba Factors affecting the induction of uncoupling protein 1 in C2C12 myogenic cells Cytokine, 2022-06-20;157(0):155936. 2022-06-20 [PMID: 35738051] (Bioassay, Mouse) Bioassay Mouse
S Yu, C Zhou, J He, Z Yao, X Huang, B Rong, H Zhu, S Wang, S Chen, X Wang, B Cai, G Zhao, Y Chen, L Xiao, H Liu, Y Qin, J Guo, H Wu, Z Zhang, M Zhang, X Zhao, F Lan, Y Wang, J Chen, S Cao, D Pei, J Liu BMP4 drives primed to na�ve transition through PGC-like state Nature Communications, 2022-05-19;13(1):2756. 2022-05-19 [PMID: 35589713] (Bioassay, Mouse) Bioassay Mouse
ML Pérez-Loza, L Sudre, S van Eegher, D Citadelle, A Pigenet, MH Lafage-Pro, P Pastoureau, F De Ceuninc, F Berenbaum, X Houard Gremlin-1 and BMP-4 Overexpressed in Osteoarthritis Drive an Osteochondral-Remodeling Program in Osteoblasts and Hypertrophic Chondrocytes International Journal of Molecular Sciences, 2022-02-14;23(4):. 2022-02-14 [PMID: 35216203] (Bioassay, Mouse) Bioassay Mouse
H Kim, BW Song, SJ Park, SW Choi, H Moon, KC Hwang, SW Kang, SH Moon, Y Yang, IC Kwon, SH Kim Ultraefficient extracellular vesicle-guided direct reprogramming of fibroblasts into functional cardiomyocytes Science Advances, 2022-02-25;8(8):eabj6621. 2022-02-25 [PMID: 35213232] (Bioassay, Mouse) Bioassay Mouse
X Hu, H Wang, GG Tian, C Hou, B Xu, X Zhao, Y Zhao, Q Fang, X Li, L He, X Chen, S Li, J Wu Offspring production of haploid spermatid-like cells derived from mouse female germline stem cells with chromatin condensation Cell & bioscience, 2022-01-04;12(1):5. 2022-01-04 [PMID: 34983631] (Bioassay, Mouse) Bioassay Mouse
D Holoch, M Wassef, C Lövkvist, D Zielinski, S Aflaki, B Lombard, T Héry, D Loew, M Howard, R Margueron A cis-acting mechanism mediates transcriptional memory at Polycomb target genes in mammals Nature Genetics, 2021-11-15;0(0):. 2021-11-15 [PMID: 34782763] (Bioassay, Mouse) Bioassay Mouse
N Hosaka, S Kanda, T Shimono, T Nishiyama Induction of gammadeltaT cells from HSC-enriched BMCs co-cultured with iPSC-derived thymic epithelial cells Journal of Cellular and Molecular Medicine, 2021-10-23;0(0):. 2021-10-23 [PMID: 34687276] (Cell Culture, Mouse) Cell Culture Mouse
SKT Ooi, H Jiang, Y Kang, P Allard Examining the Developmental Trajectory of an in Vitro Model of Mouse Primordial Germ Cells following Exposure to Environmentally Relevant Bisphenol A Levels Environmental health perspectives, 2021-09-29;129(9):97013. 2021-09-29 [PMID: 34585602] (Bioassay, Mouse) Bioassay Mouse
Show All 108 Publications.

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Bioinformatics

Gene Symbol Bmp4
Uniprot