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BMPR-II Antibody [Unconjugated]

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BMPR-II was detected in formalin fixed paraffin-embedded sections of human prostate using Goat Anti-Human BMPR-II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF811) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
BMPR-II was detected in formalin fixed paraffin-embedded sections of human prostate using Goat Anti-Human BMPR-II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF811) at 15 µg/mL overnight at 4 °C. Tissue ...read more
PC‑3 human prostate cancer cell line was stained with Goat Anti-Human BMPR‑II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF811, filled histogram) or control antibody (Catalog # AB-108-C, open ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF811
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Catalog# & Formulation Size Price
Carrier Free
AF811-SP
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BMPR-II Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human BMPR-II
Ala26-Ile151
Accession # Q13873
Specificity
Detects human BMPR-II in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
BMPR2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF811 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BMPR-II Antibody [Unconjugated]

  • BMP type II receptor
  • BMP type-2 receptor
  • BMPR2
  • BMPR-2
  • BMPR3
  • BMPRII
  • BMPR-II
  • BMPR-IIprimary pulmonary hypertension 1
  • Bone morphogenetic protein receptor type II
  • bone morphogenetic protein receptor type-2
  • bone morphogenetic protein receptor, type II (serine/threonine kinase)
  • BRK-3
  • EC 2.7.11
  • EC 2.7.11.30
  • FLJ41585
  • FLJ76945
  • PPH1BMR2
  • T-ALK
  • type II activin receptor-like kinase
  • type II receptor for bone morphogenetic protein-4

Background

Like other members of the TGF-beta superfamily, cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the binding to heteromeric complex of type I and type II serine-threonine kinase receptors. Both receptor types are required for the signal transduction. BMP receptor II (BMPR-II) is one of the five mammalian type II receptors (including TGF-beta R-II, ActR-II, ActR-IIB, BMPR-II, and MISR-II) for the TGF-beta superfamily ligands. The type II receptors for TGF-beta and activin bind ligands with high affinity by itself. In contrast, BMPR-II binds BMP-2, BMP-4, and BMP-7 weakly in the absence of type I receptor, and the binding can be facilitated by the presence of the type I receptor. BMPR-II mRNA is widely expressed in fetal and adult tissues. Human and mouse BMPR-II are highly conserved sharing 97% sequence identity (1-3).

  1. Rosenzweig, B.L. et al. (1995) Proc. Natl. Acad. Sci. USA 92:7632.
  2. Beppu, H. et al. (1997) Biochem. Biophys. Res. Commun. 235:499.
  3. Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BMPR-II Antibody (AF811)(12)

We have publications tested in 3 confirmed species: Human, Mouse, Rabbit.

We have publications tested in 4 applications: Flow Cytometry, ICC, IHC, Western Blot.


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Flow Cytometry
(1)
ICC
(2)
IHC
(2)
Western Blot
(3)
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Human
(5)
Mouse
(1)
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(1)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using AF811 Applications Species
A Ribeiro, C Freitas, L Matos, A Gouveia, F Gomes, JL Silva Carv, H Almeida Age-related expression of TGF beta family receptors in human cumulus oophorus cells J. Assist. Reprod. Genet., 2017-05-02;0(0):. 5/2/2017 [PMID: 28466233]
SV Hegarty, AM Sullivan, GW O'Keeffe Endocytosis contributes to BMP2-induced Smad signalling and neuronal growth Neurosci. Lett, 2017-02-08;643(0):32-37. 2017-02-08 [PMID: 28188849] (ICC, Human) ICC Human
Bolander J, Ji W, Geris L et al. The combined mechanism of bone morphogenetic protein- and calcium phosphate-induced skeletal tissue formation by human periosteum derived cells. Eur Cell Mater. 1/5/2016 [PMID: 26728496]
Wang Y, Kahaleh B Epigenetic repression of bone morphogenetic protein receptor II expression in scleroderma. J Cell Mol Med, 2013-07-16;17(10):1291-9. 2013-07-16 [PMID: 23859708] (Western Blot, Human) Western Blot Human
Chou H, Lai D, Huang C, McLennan I, Wang H, Wang P BMP4 is a peripherally-derived factor for motor neurons and attenuates glutamate-induced excitotoxicity in vitro. PLoS ONE, 2013-03-05;8(3):e58441. 2013-03-05 [PMID: 23472198] (IHC, Mouse) IHC Mouse
Fong D, Bisson M, Laberge G, McManus S, Grenier G, Faucheux N, Roux S Bone morphogenetic protein-9 activates Smad and ERK pathways and supports human osteoclast function and survival in vitro. Cell Signal, 2013-01-08;25(4):717-28. 2013-01-08 [PMID: 23313128] (ICC, Western Blot, Human) ICC, Western Blot Human
Joy Cogan, Eric Austin, Lora Hedges, Bethany Womack, James West, James Loyd et al. Role of BMPR2 Alternative Splicing in Heritable Pulmonary Arterial Hypertension Penetrance Circulation 2012-10-09 [PMID: 22923426]
Imran N. Mungrue, Peng Zhao, Yucheng Yao, Haijin Meng, Christoph Rau, Jocelyn V. Havel et al. Abcc6 deficiency causes increased infarct size and apoptosis in a mouse cardiac ischemia-reperfusion (I/R) model Arteriosclerosis, Thrombosis, and Vascular Biology 2011-12-01 [PMID: 21979437]
He J, Dong J, Wang T Bone morphogenetic protein receptor IB as a marker for enrichment of osteogenic precursor-like cells in human dermis. Arch. Dermatol. Res., 2011-06-05;303(8):581-90. 2011-06-05 [PMID: 21644047] (Flow Cytometry, Human) Flow Cytometry Human
R Hamid, L K Hedges, E Austin, J A Phillips, J E Loyd, J D Cogan Transcripts from a novel BMPR2 termination mutation escape nonsense mediated decay by downstream translation re-initiation: implications for treating pulmonary hypertension Clinical Genetics 2010-03-01 [PMID: 20095988]
Show All 12 Publications.

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Bioinformatics

Gene Symbol BMPR2
Uniprot