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Recombinant Mouse B7-1/CD80 Fc Chimera His-tag Protein, CF

 (10 Publications)
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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
740-B1-01M
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Recombinant Mouse B7-1/CD80 Fc Chimera His-tag Protein, CF Summary

Details of Functionality
Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909. The ED50 for this effect is 5‑30 ng/mL in the presence of PHA.
Source
Mouse myeloma cell line, NS0-derived mouse B7-1/CD80 protein
Mouse B7-1
(Asp37-Lys245)
Accession # Q00609		 DIEGRMD Human IgG1
(Pro100-Lys330) 		6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Asp37
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Cd80
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
51.3 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
80-90 kDa, reducing conditions
Publications
Read Publications using
740-B1 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile, deionized water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse B7-1/CD80 Fc Chimera His-tag Protein, CF

  • Activation B7-1 antigen
  • B7
  • B71
  • B7-1
  • BB1
  • CD28LG1
  • CD28LGB7-1 antigen)
  • CD80 antigen
  • CD80 molecule
  • CD80
  • costimulatory factor CD80
  • costimulatory molecule variant IgV-CD80
  • CTLA-4 counter-receptor B7.1
  • T-lymphocyte activation antigen CD80

Background

B7-1/CD80 and B7-2/CD86, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses (1). Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response (2-6). Mature mouse B7-1 consists of a 209 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 22 aa transmembrane segment, and a 38 aa cytoplasmic domain (7). Within the ECD, mouse B7-1 shares 50% and 68% aa sequence identity with human and rat B7-1, respectively. Alternative splicing generates a short isoform that lacks the second Ig-like domain but retains the ability to bind CD28 (8). Both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4 (1). B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells (2).
  1. Ville, S. et al. (2015) Front. Immunol. 6:411.
  2. Azuma, M. et al. (1993) Nature 366:76.
  3. Freeman, G.J. et al. (1993) Science 262:909.
  4. Chen, C. et al. (1994) J. Immunol. 152:4929.
  5. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
  6. Lanier, L. et al. (1995) J. Immunol. 154:97.
  7. Freeman, G. et al. (1991) J. Exp. Med. 174:625.
  8. Inobe, M. et al. (1994) Biochem. Biophys. Res. Commun. 200:443.

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Publications for B7-1/CD80 (740-B1)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: Bioassay, ICC, Surface Plasmon Resonance.


Filter By Application
Bioassay
(8)
ICC
(1)
Surface Plasmon Resonance
(1)
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Human
(1)
Mouse
(7)
Rat
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using 740-B1 Applications Species
J Morimoto, M Matsumoto, R Miyazawa, H Yoshida, K Tsuneyama, M Matsumoto Aire suppresses CTLA-4 expression from the thymic stroma to control autoimmunity Cell Reports, 2022-02-15;38(7):110384. 2022-02-15 [PMID: 35172142] (Bioassay, Mouse) Bioassay Mouse
J Guiteras, L De Ramon, E Crespo, N Bolaños, S Barcelo-Ba, L Martinez-V, P Fontova, M Jarque, A Torija, O Bestard, D Resina, JM Grinyó, J Torras Dual and Opposite Costimulatory Targeting with a Novel Human Fusion Recombinant Protein Effectively Prevents Renal Warm Ischemia Reperfusion Injury and Allograft Rejection in Murine Models International Journal of Molecular Sciences, 2021-01-26;22(3):. 2021-01-26 [PMID: 33530581] (Surface Plasmon Resonance, Rat) Surface Plasmon Resonance Rat
L Li, SM Jay, Y Wang, SW Wu, Z Xiao IL-12 stimulates CTLs to secrete exosomes capable of activating bystander CD8(+) T cells Sci Rep, 2017-10-17;7(1):13365. 2017-10-17 [PMID: 29042682] (Bioassay, Mouse) Bioassay Mouse
Stojanovic A, Fiegler N, Brunner-Weinzierl M, Cerwenka A CTLA-4 is expressed by activated mouse NK cells and inhibits NK Cell IFN-gamma production in response to mature dendritic cells. J Immunol, 2014-03-31;192(9):4184-91. 2014-03-31 [PMID: 24688023] (Bioassay, Mouse) Bioassay Mouse
Kloog , Yoel, Mor , Adam Cytotoxic-T-lymphocyte antigen 4 receptor signaling for lymphocyte adhesion is mediated by C3G and Rap1. Mol Cell Biol, 2014-01-06;34(6):978-88. 2014-01-06 [PMID: 24396067] (Bioassay, Human) Bioassay Human
Drees J, Augustin L, Mertensotto M, Schottel J, Leonard A, Saltzman D Soluble production of a biologically active single-chain antibody against murine PD-L1 in Escherichia coli. Protein Expr Purif, 2013-11-08;94(0):60-6. 2013-11-08 [PMID: 24215864] (Bioassay, Mouse) Bioassay Mouse
Hammerbeck CD, Mescher MF Antigen controls IL-7Ralpha expression levels on CD8 T cells during full activation or tolerance induction. J. Immunol., 2008-02-15;180(4):2107-16. 2008-02-15 [PMID: 18250416] (Bioassay, Mouse) Bioassay Mouse
Butte MJ, Keir ME, Phamduy TB, Sharpe AH, Freeman GJ Programmed death-1 ligand 1 interacts specifically with the B7-1 costimulatory molecule to inhibit T cell responses. Immunity, 2007-07-12;27(1):111-22. 2007-07-12 [PMID: 17629517] (Bioassay) Bioassay
Pentcheva-Hoang T, Egen JG, Wojnoonski K, Allison JP B7-1 and B7-2 selectively recruit CTLA-4 and CD28 to the immunological synapse. Immunity, 2004-09-01;21(3):401-13. 2004-09-01 [PMID: 15357951] (ICC, Mouse) ICC Mouse
Shin T, Kennedy G, Gorski K, Tsuchiya H, Koseki H, Azuma M, Yagita H, Chen L, Powell J, Pardoll D, Housseau F Cooperative B7-1/2 (CD80/CD86) and B7-DC costimulation of CD4+ T cells independent of the PD-1 receptor. J. Exp. Med., 2003-07-07;198(1):31-8. 2003-07-07 [PMID: 12847135] (Bioassay, Mouse) Bioassay Mouse

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Additional B7-1/CD80 Products

Blogs on B7-1/CD80.

Check out the latest blog posts on B7-1/CD80.
CD80: A co-stimulator of T cell activation
CD80 is a 60kD single chain type I transmembrane glycoprotein that is a member of the immunoglobulin family. CD80 is expressed on activated B- and T-lymphocytes, as well as a subpopulation of previously activated B-cells, but not on the majority of re...  Read full blog post.
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Bioinformatics

Gene Symbol Cd80
Uniprot