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Recombinant Human VEGF (aa 207-318) Protein, CF

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Recombinant Human VEGF (aa 207-318) (Catalog # 298-VS/CF) stimulates cell proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner. The ED50 is 0.75-3.75 ng/mL.
Recombinant Human VEGF (aa 207-318) (Catalog # 298-VS/CF) stimulates cell proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner. The ED50 is 0.75-3.75 ng/mL.
1 μg/lane of Recombinant Human VEGF (aa 207-318) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 13 kDa and 36 kDa, respectively.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
298-VS-025/CF
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Recombinant Human VEGF (aa 207-318) Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 0.75-3.75 ng/mL.
Source
E. coli-derived human VEGF protein
Ala207-Arg318
Accession #
N-terminal Sequence
Ala207
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
VEGFA
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
14.1 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
298-VS/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 50 µg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human VEGF (aa 207-318) Protein, CF

  • MVCD1
  • VAS
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • Vasculotropin
  • VEGF
  • VEGFA
  • VEGF-A
  • VEGFMGC70609
  • VPF
  • VPFvascular endothelial growth factor

Background

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1 - 3). It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cysteine knot structure (4). Humans express alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) in length (4). VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189 (3, 4). VEGF121 is the only form that lacks a basic heparin-binding region and is freely diffusible (4). Mouse embryos expressing only the corresponding isoform (VEGF120) do not survive to term, and show defects in skeletogenesis (5). Human VEGF121 shares 87% aa sequence identity with corresponding regions of mouse and rat, 93% with feline, equine and bovine, and 91%, 95% and 96% with ovine, canine and porcine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells (4). Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). VEGF165 binds the semaphorin receptor, Neuropilin-1; VEGF121 binding has also been reported (6). VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells (3, 4). In adults, VEGF functions mainly in wound healing and the female reproductive cycle (3). Pathologically, it is involved in tumor angiogenesis and vascular leakage (7, 8). Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus (9). VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M and TNF-alpha (3, 4, 10).

  1. Leung, D.W. et al. (1989) Science 246:1306. 
  2. Keck, P.J. et al. (1989) Science 246:1309. 
  3. Byrne, A.M. et al. (2005) J. Cell. Mol. Med. 9:777.
  4. Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853.
  5. Zelzer, E. et al. (2002) Development 129:1893.
  6. Pan, Q. et al. (2007) J. Biol. Chem. 282:24049.
  7. Weis, S.M. and D.A. Cheresh (2005) Nature 437:497.
  8. Thurston, G. (2002) J. Anat. 200:575.
  9. Carvalho, J.F. et al. (2007) J. Clin. Immunol. 27:246.
  10. Angelo, L.S. and R. Kurzrock (2007) Clin. Cancer Res. 13:2825.

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Recombinant Human
VEGF (aa 207-318) Pro ...
298-VS/CF
Recombinant Human VEGF (aa 207-318) Protein, CF
Species: Hu
Applications: Bioactivity

Publications for VEGF (298-VS/CF)(12)

We have publications tested in 2 confirmed species: Human, Bovine.

We have publications tested in 4 applications: Binding Assay, Bioassay, ELISA (Standard), Surface Plasmon Resonance (SPR.


Filter By Application
Binding Assay
(1)
Bioassay
(10)
ELISA (Standard)
(1)
Surface Plasmon Resonance (SPR
(1)
All Applications
Filter By Species
Human
(10)
Bovine
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using 298-VS/CF Applications Species
S Li, CS Wang, A Montel-Hag, HC Chen, S Lopez, O Zhou, K Dai, S Tsai, W Satyadi, C Botero, C Wong, D Casero, GM Crooks, CS Seet Strength of CAR signaling determines T�cell versus ILC differentiation from pluripotent stem cells Cell Reports, 2023-03-11;42(3):112241. 2023-03-11 [PMID: 36906850] (Bioassay, Human) Bioassay Human
A Montel-Hag, CS Seet, S Li, B Chick, Y Zhu, P Chang, S Tsai, V Sun, S Lopez, HC Chen, C He, CJ Chin, D Casero, GM Crooks Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells Cell Stem Cell, 2019-01-17;0(0):. 2019-01-17 [PMID: 30661959] (Bioassay, Human) Bioassay Human
N Seo, A Polozova, M Zhang, Z Yates, S Cao, H Li, S Kuhns, G Maher, HJ McBride, J Liu Analytical and functional similarity of Amgen biosimilar ABP 215 to bevacizumab MAbs, 2018-04-20;0(0):1-58. 2018-04-20 [PMID: 29553864] (Bioassay, Surface Plasmon Resonance (SPR, Human) Bioassay, Surface Plasmon Resonance (SPR Human
Zacchigna S, Pattarini L, Zentilin L, Moimas S, Carrer A, Sinigaglia M, Arsic N, Tafuro S, Sinagra G, Giacca M Bone marrow cells recruited through the neuropilin-1 receptor promote arterial formation at the sites of adult neoangiogenesis in mice. J. Clin. Invest., 2008-06-01;118(6):2062-75. 2008-06-01 [PMID: 18483621] (Bioassay, Human) Bioassay Human
Matsuda A, Fukuda S, Matsumoto K, Saito H Th1/Th2 cytokines reciprocally regulate in vitro pulmonary angiogenesis via CXC chemokine synthesis. Am. J. Respir. Cell Mol. Biol., 2007-08-20;38(2):168-75. 2007-08-20 [PMID: 17709600] (Bioassay, Human) Bioassay Human
Pan Q, Chathery Y, Wu Y, Rathore N, Tong RK, Peale F, Bagri A, Tessier-Lavigne M, Koch AW, Watts RJ Neuropilin-1 binds to VEGF121 and regulates endothelial cell migration and sprouting. J. Biol. Chem., 2007-06-16;282(33):24049-56. 2007-06-16 [PMID: 17575273] (Binding Assay, Human) Binding Assay Human
Ball SG, Shuttleworth CA, Kielty CM Vascular endothelial growth factor can signal through platelet-derived growth factor receptors. J. Cell Biol., 2007-04-30;177(3):489-500. 2007-04-30 [PMID: 17470632] (Bioassay, Human) Bioassay Human
Neria F, Caramelo C, Peinado H, Gonzalez-Pacheco FR, Deudero JJ, de Solis AJ, Fernandez-Sanchez R, Penate S, Cano A, Castilla MA Mechanisms of endothelial cell protection by blockade of the JAK2 pathway. Am. J. Physiol., Cell Physiol., 2006-10-11;292(3):C1123-31. 2006-10-11 [PMID: 17035297] (Bioassay, Human) Bioassay Human
Lake AC, Vassy R, Di Benedetto M, Lavigne D, Le Visage C, Perret GY, Letourneur D Low molecular weight fucoidan increases VEGF165-induced endothelial cell migration by enhancing VEGF165 binding to VEGFR-2 and NRP1. J. Biol. Chem., 2006-10-06;281(49):37844-52. 2006-10-06 [PMID: 17028197] (Bioassay, Human) Bioassay Human
Narazaki M, Tosato G Ligand-induced internalization selects use of common receptor neuropilin-1 by VEGF165 and semaphorin3A. Blood, 2006-01-19;107(10):3892-901. 2006-01-19 [PMID: 16424390] (ELISA (Standard)) ELISA (Standard)
Show All 12 Publications.

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FAQs for VEGF (298-VS/CF). (Showing 1 - 1 of 1 FAQs).

  1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
    • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

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Getting Physical: Link between Lipid Metabolism and Hypoxia Target Genes
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Bioinformatics

Gene Symbol VEGFA
Uniprot