Recombinant Human MIS/AMH Protein

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Müllerian inhibiting substance (MIS), also named anti‑Müllerian hormone (AMH), is a tissue-specific TGF-beta superfamily growth factor. Its expression is restricted to the Sertoli cells of fetal and postnatal testis, and to the granulosa cells of postnatal ovary (1). The human MIS gene encodes a 553 amino acid residue (aa) prepropeptide containing a signal a sequence (1-24), a pro‑region (25-455), and the carboxyl-terminal bioactive protein (446-553) (2‑4). MIS is synthesized and secreted as a disulfide-linked homodimeric pro‑protein. Proteolytic cleavage is required to generate the N-terminal pro‑region and the C‑terminal bioactive protein, which remain associated in a non-covalent complex. Recombinant C‑terminal MIS has been shown to be bioactive. However, the complex with the N-terminal pro‑region showed enhanced activity (3, 5). The C‑terminal region contains the seven canonical cysteine residues found in TGF-beta  superfamily members. These cysteine residues are involved in inter- and intra-molecular disulfide bonds, which forms the cysteine knot structure. Human and mouse MIS share 73% and 90% aa sequence identity in their pro‑region and C‑terminal region, respectively. MIS induces Mullerian duct (female reproductive tract) regression during sexual differentiation in the male embryo (6). Posnatally, MIS has been shown to regulate gonadal functions (1). MIS inhibits Leydig cell proliferation and is a regulator of the initial and cyclic recruitment of ovarian follicles. MIS has also been found to have anti‑proliferative effects on breast, ovarian and prostate tumor cells (7-9).

Like other TGF-beta superfamily members, MIS signals via a heteromeric receptor complex consisting of a type I and a type II receptor serine/threonine kinase. Depending on the cell context, different type I receptors (including Act RIA/ALK2, BMP RIA/ALK3, and BMP RIB/ALK6) that are shared by other TGF-beta superfamily members, have been implicated in MIS signaling (10 - 12). In contrast, the type II MIS receptor (MIS RII) is unique and does not bind other TGF-beta superfamily members. Upon ligand binding, MIS RII recruits the non-ligand binding type I receptor into the complex, resulting in phosphorylation the BMP-like signaling pathway effector proteins Smad1, Smad5 and Smad 8 (10‑12).

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Primate - Chlorocebus aethiops (African Green Monkey), Primate - Macaca nemestrina (Southern Pig-tailed Macaque), Sea bass.

We have publications tested in 6 applications: Antibody Absorption, Bioassay, Cell Culture, In Vivo, Organoid Culture, Surface Plasmon Resonance.

Showing Publications 1 - 10 of 18. Show All 18 Publications. Collapse Publications.

Publications using 1737-MS	Applications	Species
Tarabeih, R;Nemerovsky, L;Bar-Joseph, H;Eldar-Boock, A;Elmechaly, CL;Ben-Ami, I;Shalgi, R; Pigment epithelium-derived factor expression and role in follicular development Reproductive biomedicine online 2024-04-04 [PMID: 38870625] (Bioassay, Human)	Bioassay	Human
M Chen, X Guo, Y Zhong, Y Liu, B Cai, R Wu, C Huang, C Zhou AMH inhibits androgen production in human theca cells The Journal of steroid biochemistry and molecular biology, 2022-11-07;226(0):106216. 2022-11-07 [PMID: 36356855] (Bioassay, Human)	Bioassay	Human
K Wang, F Xu, J Maylie, J Xu Anti-M�llerian Hormone Regulation of Synaptic Transmission in the Hippocampus Requires MAPK Signaling and Kv4.2 Potassium Channel Activity Frontiers in Neuroscience, 2021-12-16;15(0):772251. 2021-12-16 [PMID: 34975379] (Bioassay, Mouse)	Bioassay	Mouse
C Zapater, A Rocha, G Molés, A Mascoli, S Ibañez, S Zanuy, A Gómez Functional Activity of Recombinant Forms of Amh and Synergistic Action with Fsh in European Sea Bass Ovary International Journal of Molecular Sciences, 2021-09-18;22(18):. 2021-09-18 [PMID: 34576257] (Bioassay, Sea bass)	Bioassay	Sea bass
T Tumurbaata, H Kanasaki, Z Tumurgan, A Oride, H Okada, S Kyo Effect of anti-M�llerian hormone on the regulation of pituitary gonadotropin subunit expression: roles of kisspeptin and its receptors in gonadotroph L&betaT2 cells Endocrine journal, 2021-05-15;0(0):. 2021-05-15 [PMID: 33994401] (Bioassay, Mouse)	Bioassay	Mouse
M Peigné, P Pigny, MW Pankhurst, E Drumez, A Loyens, D Dewailly, S Catteau-Jo, P Giacobini The proportion of cleaved anti-M�llerian hormone is higher in serum but not follicular fluid of obese women independently of polycystic ovary syndrome Reprod Biomed Online, 2020-07-25;0(0):. 2020-07-25 [PMID: 33046375] (Human)		Human
Y Li, D Gao, T Xu, MK Adur, L Zhang, L Luo, T Zhu, X Tong, D Zhang, Y Wang, W Ning, X Qi, Z Cao, Y Zhang Anti-M�llerian hormone inhibits luteinizing hormone-induced androstenedione synthesis in porcine theca cells Theriogenology, 2019-11-02;142(0):421-432. 2019-11-02 [PMID: 31711705] (Cell Culture, Human)	Cell Culture	Human
SA Malone, GE Papadakis, A Messina, NEH Mimouni, S Trova, M Imbernon, C Allet, I Cimino, J Acierno, D Cassatella, C Xu, R Quinton, G Szinnai, P Pigny, L Alonso-Cot, L Masgrau, JD Maréchal, V Prevot, N Pitteloud, P Giacobini Defective AMH signaling disrupts GnRH neuron development and function and contributes to hypogonadotropic hypogonadism Elife, 2019-07-10;8(0):. 2019-07-10 [PMID: 31291191] (Bioassay, Mouse, Primate - Chlorocebus aethiops (African Green Monkey))	Bioassay	Mouse, Primate - Chlorocebus aethiops (African Green Monkey)
B Tata, NEH Mimouni, AL Barbotin, SA Malone, A Loyens, P Pigny, D Dewailly, S Catteau-Jo, I Sundström-, TT Piltonen, F Dal Bello, C Medana, V Prevot, J Clasadonte, P Giacobini Elevated prenatal anti-M�llerian hormone reprograms the fetus and induces polycystic ovary syndrome in adulthood Nat. Med., 2018-05-14;0(0):. 2018-05-14 [PMID: 29760445] (In Vivo, Mouse)	In Vivo	Mouse
MW Pankhurst, RL Kelley, RL Sanders, SR Woodcock, DE Oorschot, NJ Batchelor Anti-M�llerian hormone overexpression restricts preantral ovarian follicle survival J. Endocrinol., 2018-03-14;0(0):. 2018-03-14 [PMID: 29540452] (Bioassay, Mouse)	Bioassay	Mouse
A Yamamoto, T Omotehara, Y Miura, T Takada, N Yoneda, T Hirano, Y Mantani, H Kitagawa, T Yokoyama, N Hoshi The mechanisms underlying the effects of AMH on M�llerian duct regression in male mice J. Vet. Med. Sci., 2018-03-09;0(0):. 2018-03-09 [PMID: 29526868] (Organoid Culture, Mouse)	Organoid Culture	Mouse
Xu J, Bishop C, Lawson M, Park B, Xu F Anti-Mullerian hormone promotes pre-antral follicle growth, but inhibits antral follicle maturation and dominant follicle selection in primates. Hum Reprod, 2016-05-09;31(7):1522-30. 2016-05-09 [PMID: 27165618] (Bioassay, Primate - Macaca nemestrina (Southern Pig-tailed Macaque))	Bioassay	Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
Signorile P, Petraglia F, Baldi A Anti-mullerian hormone is expressed by endometriosis tissues and induces cell cycle arrest and apoptosis in endometriosis cells. J Exp Clin Cancer Res, 2014-05-29;33(0):46. 2014-05-29 [PMID: 24886254] (Bioassay, Human)	Bioassay	Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human)	Bioassay	Human
Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth. J. Biol. Chem., 2011-07-07;286(34):30034-46. 2011-07-07 [PMID: 21737454] (Surface Plasmon Resonance, Human, Mouse)	Surface Plasmon Resonance	Human, Mouse
Nilsson EE, Schindler R, Savenkova MI, Skinner MK Inhibitory actions of Anti-Mullerian Hormone (AMH) on ovarian primordial follicle assembly. PLoS ONE, 2011-05-27;6(5):e20087. 2011-05-27 [PMID: 21637711] (Bioassay, Rat)	Bioassay	Rat
Herrera B, Inman GJ A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum. BMC Cell Biol., 2009-03-19;10(0):20. 2009-03-19 [PMID: 19298647] (Bioassay, Mouse)	Bioassay	Mouse
Wang J, Dicken C, Lustbader JW, Tortoriello DV Evidence for a Mullerian-inhibiting substance autocrine/paracrine system in adult human endometrium. Fertil. Steril., 2008-03-07;91(4):1195-203. 2008-03-07 [PMID: 18328480] (Antibody Absorption, Bioassay, Human)	Antibody Absorption, Bioassay	Human
Show All 18 Publications. Collapse Publications.

Array 1737-MS

• MIS/AMH Antibodies
• MIS/AMH ELISA Kits
• MIS/AMH Lysate
• MIS/AMH Protein
• MIS/AMH Proteins and Enzymes
• MIS/AMH ELISA