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Recombinant Human Chemerin Protein

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human Chemerin Protein Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human ChemR23. The ED50 for this effect is 4-20 ng/mL.
Source
E. coli-derived human Chemerin protein
Glu21-Ser157, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
RARRES2
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
2324-CM in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Chemerin Protein

  • Chemerin
  • RARRES2
  • RAR-responsive protein TIG2
  • retinoic acid receptor responder (tazarotene induced) 2
  • retinoic acid receptor responder protein 2
  • Tazarotene-induced gene 2 protein
  • TIG-2
  • TIG2HP10433

Background

Human Chemerin, also known as Tazarotene-induced Gene 2, (TIG2) is a new, but distant member of the Cystatin superfamily (1 - 3). Members of this superfamily contain at least two intrachain disulfide bonds and an alpha -helical structure over a distance of about 100 amino acids (2, 3). Chemerin is synthesized as a 163 aa precursor that contains a hydrophobic 20 aa N-terminal sequence, an intervening 137 aa Cystatin-fold containing domain, and a six aa C‑terminal prosegment (1, 4). Within the cystatin-fold domain there are three intrachain disulfide bonds that contribute to the fold, and three potential sites for phosphorylation and one for myristoylation (5). The precursor molecule undergoes proteolytic processing at both termini by unknown proteases. The N-terminal residue 20 aa hydrophobic segment is described as being either a signal sequence or a transmembrane (TM) segment for a type II TM protein (1, 6). In either case, it gives rise to a soluble proform that undergoes further processing at the C‑terminus. In human, the C‑terminal six residues are cleaved, giving rise to a monomeric, 16 kDa heparin-binding bioactive molecule (aa 21 - 157) (7). A shorter 134 aa form has been described (5). Bioactivity seems to be concentrated in the nine residues preceding the prosegment (aa 149 - 157). Retention of the prosegment blocks activity (4). The 137 aa mature segment is known to bind to the G-protein coupled receptor termed ChemR23 (5, 7). Binding results in macrophage and immature dendritic cell chemotaxis (7). The distribution of this receptor is limited to immune APCs, and it is assumed that Chemerin is an inflammatory molecule. It is unclear which cells are actually producing Chemerin, but keratinocytes, endothelial cells and osteoclasts are potential candidates (1, 7). Mature human Chemerin shares 67% aa sequence identity with mouse Chemerin (7). There is apparently cross-species activity for the protein (8).

  1. Nagpal, S. et al. (1997) J. Invest. Dermatol. 109:91.
  2. Storici, P. et al. (1996) Eur. J. Biochem. 238:769.
  3. Zanetti, M. (2004) J. Leukoc. Biol. 75:39.
  4. Wittamer, V. et al. (2004) J. Biol. Chem. 279:9956.
  5. Meder, W. et al. (2003) FEBS Lett. 555:495.
  6. Yokoyama-Kobayashi, M. et al. (1999) Gene 228:161.
  7. Wittamer, V. et al. (2003) J. Exp. Med. 198:977.
  8. Busmann, A. et al. (2004) J. Chromatog. B 811:217.

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2324-CM
Species: Hu
Applications: Bioactivity

Publications for Chemerin (2324-CM)(13)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Bioassay, ELISA (Standard), Western Blot.


Filter By Application
Bioassay
(11)
ELISA (Standard)
(2)
Western Blot
(1)
All Applications
Filter By Species
Human
(10)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using 2324-CM Applications Species
Moreira, MLM;de Araújo, IM;Fukada, SY;Venturini, LGR;Guidorizzi, NR;Garrido, CE;Rosen, CJ;de Paula, FJA; Refining Evaluation of Bone Mass and Adipose Distribution in Dunnigan Syndrome International journal of molecular sciences 2023-08-23 [PMID: 37685926] (Bioassay, Human) Bioassay Human
Y Chen, Z Liu, P Gong, H Zhang, Y Chen, S Yao, W Li, Y Zhang, Y Yu The Chemerin/CMKLR1 Axis Is Involved in the Recruitment of Microglia to Abeta Deposition through p38 MAPK Pathway International Journal of Molecular Sciences, 2022-08-12;23(16):. 2022-08-12 [PMID: 36012305] (Bioassay, Mouse) Bioassay Mouse
I Dubois-Ved, D Al Delbany, O De Henau, V Robert, M Vernimmen, F Langa, A Lefort, F Libert, V Wittamer, M Parmentier The antitumoral effects of chemerin are independent from leukocyte recruitment and mediated by inhibition of neoangiogenesis Oncotarget, 2021-09-14;12(19):1903-1919. 2021-09-14 [PMID: 34548907] (Bioassay, Human) Bioassay Human
V Kumar, M LaJevic, M Pandrala, SA Jacobo, SV Malhotra, BA Zabel Novel CMKLR1 Inhibitors for Application in Demyelinating Disease Sci Rep, 2019-05-09;9(1):7178. 2019-05-09 [PMID: 31073181] (Bioassay, Human) Bioassay Human
J Shang, L Wang, Y Zhang, S Zhang, L Ning, J Zhao, G Cheng, D Liu, J Xiao, Z Zhao Chemerin/ChemR23 axis promotes inflammation of glomerular endothelial cells in diabetic nephropathy J. Cell. Mol. Med., 2019-02-19;0(0):. 2019-02-19 [PMID: 30784180] (Bioassay, Human) Bioassay Human
A Momenpour, PDA Lima, YA Chen, CR Tzeng, BK Tsang, H Anis Surface-enhanced Raman scattering for the detection of polycystic ovary syndrome Biomed Opt Express, 2018-01-25;9(2):801-817. 2018-01-25 [PMID: 29552414] (Bioassay, Human) Bioassay Human
Kerry B Goralski CMKLR1 activation ex vivo does not increase proportionally to serum total chemerin in obese humans Endocr Connect, 2016-11-08;5(6):70-81. 2016-11-08 [PMID: 27881447] (Bioassay, Human) Bioassay Human
JD Kumar, S Kandola, L Tiszlavicz, Z Reisz, GJ Dockray, A Varro The role of chemerin and ChemR23 in stimulating the invasion of squamous oesophageal cancer cells Br. J. Cancer, 2016-04-19;114(10):1152-9. 2016-04-19 [PMID: 27092781] (Bioassay, Human) Bioassay Human
Kumar , J Dinesh, Holmberg , Chris, Kandola , Sandhir, Steele , Islay, Hegyi , Peter, Tiszlavicz , Laszlo, Jenkins , Rosalind, Beynon , Robert J, Peeney , David, Giger , Olivier, Alqahtani , Ahlam, Wang , Timothy, Charvat , Trevor T, Penfold , Mark, Dockray , Graham J, Varro , Andrea Increased expression of chemerin in squamous esophageal cancer myofibroblasts and role in recruitment of mesenchymal stromal cells. PLoS ONE, 2014-08-15;9(7):e104877. 2014-08-15 [PMID: 25127029] (Bioassay, Human) Bioassay Human
Doyle J, Krishnaji S, Zhu G, Xu Z, Heller D, Ji R, Levy B, Kumar K, Kopin A Development of a membrane-anchored chemerin receptor agonist as a novel modulator of allergic airway inflammation and neuropathic pain. J Biol Chem, 2014-03-21;289(19):13385-96. 2014-03-21 [PMID: 24659779] (Bioassay, Human) Bioassay Human
Show All 13 Publications.

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Bioinformatics

Gene Symbol RARRES2
Uniprot