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Intelectin-1/Omentin Antibody [Unconjugated]

 (8 Publications)
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Intelectin‑1/Omentin was detected in immersion fixed frozen sections of mouse intestine using 10 µg/mL Sheep Anti-Human Intelectin‑1/Omentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4254) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Intelectin‑1/Omentin was detected in immersion fixed frozen sections of mouse intestine using 10 µg/mL Sheep Anti-Human Intelectin‑1/Omentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4254) ...read more
Intelectin-1/Omentin was detected in immersion fixed U937 human histiocytic lymphoma cell line using Sheep Anti-Human Intelectin-1/Omentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4254) at 5 µg/mL for ...read more
Intelectin‑1/Omentin was detected in immersion fixed sections of ileum ALI using Sheep Anti-Human Intelectin‑1/Omentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4254) at ...read more
SPINK4 and ITLN1 mark early goblet cells. a Volcano plot showing fold change and P value of genes differentially expressed in the ‘goblet-type’ cell cluster as compared to all other cell clusters (see Fig. 3). ...read more
SPINK4 and ITLN1 mark early goblet cells. a Volcano plot showing fold change and P value of genes differentially expressed in the ‘goblet-type’ cell cluster as compared to all other cell clusters (see Fig. 3). ...read more
SPINK4 and ITLN1 mark early goblet cells. a Volcano plot showing fold change and P value of genes differentially expressed in the ‘goblet-type’ cell cluster as compared to all other cell clusters (see Fig. 3). ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4254-SP
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Intelectin-1/Omentin Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Intelectin-1/Omentin
Thr19-Ser298
Accession # Q8WWA0
Specificity
Detects human Intelectin-1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
ITLN1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF4254 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Intelectin-1/Omentin Antibody [Unconjugated]

  • Endothelial lectin HL-1
  • FLJ20022
  • Galactofuranose-binding lectin
  • hIntL
  • HL-1
  • intelectin 1 (galactofuranose binding)
  • Intelectin1
  • Intelectin-1
  • INTLHL1
  • ITLN1
  • Itlna
  • ITLNITLN-1
  • LFR
  • LFRIntestinal lactoferrin receptor
  • Omentin
  • Omentin-1

Background

Intelectin-1 (intestinal lectin; also ITLN-1 and omentin) is a 40 kDa Ca-dependent galactofuranose-binding lectin that is not a C-type lectin. It is expressed on multiple cell types and appears to participate in insulin signaling and microbe recognition. The human ITLN-1 preproprecursor is 313 amino acids (aa) in length. It contains an 18 aa signal sequence, a 280 aa mature segment (aa 19-298), and a 15 aa C-terminal proregion that is cleaved to generate a GPI-linkage. One fibrinogen-related domain exists in the mature molecule (aa 36-209). Human ITLN-1 exists as a disulfide-linked glycosylated homotrimer. Mouse ITLN-1 is unglycosylated and a monomer. Mature human ITLN-1 is 82% aa identical to mouse ITLN-1. Human ITLN-1 shows one alternate start site at Met72, and a splice form that contains a 32 aa substitution for aa 264-313.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Intelectin-1/Omentin Antibody (AF4254)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 2 applications: IHC-P, Western Blot.


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(1)
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(3)
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(1)
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Showing Publications 1 - 8 of 8.
Publications using AF4254 Applications Species
Juan D. Matute, Jinzhi Duan, Magdalena B. Flak, Paul Griebel, Jose A. Tascon-Arcila, Shauni Doms et al. Intelectin-1 binds and alters the localization of the mucus barrier-modifying bacterium Akkermansia muciniphila J Exp Med 2023-01-02 [PMID: 36413219]
A Barbe, P Kurowska, E Mlyczy?ska, C Ramé, C Staub, E Venturi, Y Billon, A Rak, J Dupont Adipokines expression profiles in both plasma and peri renal adipose tissue in Large White and Meishan sows: a possible involvement in the fattening and the onset of puberty Gen. Comp. Endocrinol., 2020-08-19;0(0):113584. 2020-08-19 [PMID: 32827511] (Western Blot, Porcine) Western Blot Porcine
M Howell, DG Dumitrescu, LR Blankenshi, D Herkert, SK Hatzios Functional characterization of a subtilisin-like serine protease from Vibrio cholerae J. Biol. Chem., 2019-05-10;0(0):. 2019-05-10 [PMID: 31076508] (Western Blot, Human) Western Blot Human
Richard Peter Owen, Michael Joseph White, David Tyler Severson, Barbara Braden, Adam Bailey, Robert Goldin et al. Single cell RNA-seq reveals profound transcriptional similarity between Barrett’s oesophagus and oesophageal submucosal glands Nature Communications 2018-10-15 [PMID: 30323168]
Stavroula K. Hatzios, Sören Abel, Julianne Martell, Troy Hubbard, Jumpei Sasabe, Diana Munera et al. Chemoproteomic profiling of host and pathogen enzymes active in cholera Nature Chemical Biology 2016-04-01 [PMID: 26900865]
Darryl A. Wesener, Kittikhun Wangkanont, Ryan McBride, Xuezheng Song, Matthew B. Kraft, Heather L. Hodges et al. Recognition of microbial glycans by human intelectin-1 Nature Structural & Molecular Biology 2015-08-01 [PMID: 26148048]
Greulich , Sabrina, Chen , Weena J, Maxhera , Bujar, Rijzewijk , Luuk J, van der Meer , Rutger W, Jonker , Jacqueli, Mueller , Heidi, de Wiza , Daniella, Floerke , Ralf-Rue, Smiris , Konstant, Lamb , Hildo J, de Roos , Albert, Bax , Jeroen J, Romijn , Johannes, Smit , Jan W A, Akhyari , Payam, Lichtenberg , Artur, Eckel , Juergen, Diamant , Michaela, Ouwens , D Margri Cardioprotective properties of omentin-1 in type 2 diabetes: evidence from clinical and in vitro studies. PLoS ONE, 2013-03-29;8(3):e59697. 2013-03-29 [PMID: 23555749] (Western Blot, Human) Western Blot Human
Bahaie NS, Kang BN, Frenzel EM, Hosseinkhani MR, Ge XN, Greenberg Y, Ha SG, Demetriou M, Rao SP, Sriramarao P N-Glycans differentially regulate eosinophil and neutrophil recruitment during allergic airway inflammation. J. Biol. Chem., 2011-09-12;286(44):38231-41. 2011-09-12 [PMID: 21911487] (IHC-P, Mouse) IHC-P Mouse

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Bioinformatics

Gene Symbol ITLN1
Uniprot