Recombinant Human CD36/SR-B3 Fc Chimera Protein, CF Summary
Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human CD36/Fc Chimera is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Human TSP-2/His that producecs 50% of the optimal binding response is fround to be 15-120 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CD36/SR-B3 protein
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.
Applications/Dilutions
Dilutions
Binding Activity
Theoretical MW
73 kDa (monomer). Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
53-64 kDa & 93 kDa, under reducing conditions.
Publications
Read Publications using 1955-CD in the following applications:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human CD36/SR-B3 Fc Chimera Protein, CF
BDPLT10
CD_antigen: CD36
CD36 antigen (collagen type I receptor, thrombospondin receptor)
CD36 antigen
CD36 molecule (thrombospondin receptor)
CD36
CHDS7
cluster determinant 36
Collagen R
FAT
Fatty acid translocase
Glycoprotein IIIb
GP3B
GP4
GPIIIb
GPIV
PAS IV
PAS-4 protein
PAS-4
PASIV
Platelet collagen receptor
platelet glycoprotein 4
SCARB3
scavenger receptor class B, member 3
SRB3
SR-B3
Thrombospondin R
Thrombospondin receptor
Background
CD36, alternatively known as platelet membrane glycoprotein IV (GPIV), GPIIIb, thrombospondin receptor, collagen receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3), is an integral membrane glycoprotein that has multiple physiological functions (1). It is broadly expressed on a variety of cell types including microvascular endothelium, adipocytes, skeletal muscle, epithelial cells of the retina, breast, and intestine, smooth muscle cells, erythroid precursors, platelets, megakaryocytes, dendritic cells, monocytes/macrophages, and microglia (1, 2). As a member of the scavenger receptor family, CD36 is a multiligand pattern recognition receptor that interacts with a large number of structurally dissimilar ligands, including long chain fatty acid (LCFA), advanced glycation end products (AGE), thrombospondin-1, oxidized low-density lipoproteins (oxLDLs), high density lipoprotein (HDL), phosphatidylserine, apoptotic cells, beta -amyloid fibrils (fA beta ), collagens I and IV, and Plasmodium falciparum-infected erythrocytes (3). CD36 is required for the anti-angiogenic effects of thrombospondin-1 in the corneal neovascularization assay (4). It plays a role in lipid metabolism and has been identified as a fatty acid translocase necessary for the binding and transport of LCFA in cells and tissues (5). CD36 has been implicated in the clearance of apoptotic cells and cell debris and has also been shown to mediate the internalization and degradation of a variety of its ligands such as oxLDL, AGE and fA beta (3). Upon ligand binding, CD36 transduces signals that mediate a wide range of pro-inflammatory cellular responses (2). CD36 plays a significant role in the initiation and pathogenesis of chronic inflammatory diseases such as Alzheimer’s disease and atherosclerosis (2, 3). The human CD36 gene encodes a single-chain 472 amino acid residue protein containing both an N- and a C-terminal cytoplasmic tail and an extracellular loop.
Febbraio, M. et al. (2001) J. Clin. Invest. 108:785.
Khoury, J. et al. (2003) J. Exp. Med. 197:1657.
Husemann, J. et al. (2002) Glia 40:195.
Armstrong, L and P. Bornstein (2003) Matrix. Biol. 22:63.
Febbraio M. et al. (1999) J. Biol. Chem. 274:19055.
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