Home » CCL8/MCP-2 » CCL8 Peptides and Proteins » Recombinant Human CCL8/MCP-2 Protein, CF
Novus Biologicals products are now on bio-techne.com

Recombinant Human CCL8/MCP-2 Protein, CF

 (9 Publications)
 See All Related
Submit a Review

Images

 
There are currently no images for CCL8/MCP-2 (281-CP/CF).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
281-CP-010
Carrier Free
281-CP-050/CF
100% Guarantee on every product

Recombinant Human CCL8/MCP-2 Protein, CF Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR5. The ED50 for this effect is 0.01‑0.06 µg/mL. Measured by its ability to chemoattract THP‑1 human acute monocytic leukemia cells. The ED50 for this effect is 0.03‑0.1 µg/mL.
Source
E. coli-derived human CCL8/MCP-2 protein
Gln24-Pro99
Accession #
N-terminal Sequence
Gln24
Protein/Peptide Type
Recombinant Proteins
Gene
CCL8
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Bioactivity2
Theoretical MW
9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
281-CP/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL8/MCP-2 Protein, CF

  • C-C motif chemokine 8
  • CCL8
  • chemokine (C-C motif) ligand 8
  • HC14
  • MCP2
  • MCP-2
  • member 8 (monocyte chemotactic protein 2)
  • monocyte chemoattractant protein 2
  • SCYA10
  • small-inducible cytokine A8

Background

MCP-2 and MCP-3 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and MCP-3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. MCP-3 also shares 58% amino acid identity with MCP-2.

Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Customers Who Viewed This Item Also Viewed...

CCL2/JE/MCP-1 [HRP]
DCP00
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
(250 Publications)
Species: Hu
Applications: ELISA
CCL7/MCP-3/MARC
Antibody [Unconjugate
AF-456-NA
Neutralization CCL7/MCP-3/MARC Antibody [Unconjugated]
(15 Publications)
Species: Mu
Applications: Neut, WB
CCL5/RANTES [HRP]
DRN00B
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
(99 Publications)
Species: Hu
Applications: ELISA
CCL13/MCP-4
[Biotin]
DY327
N/A CCL13/MCP-4 [Biotin]
(12 Publications)
Species: Hu
Applications: ELISA
CCL11/Eotaxin [HRP]
MME00
N/A CCL11/Eotaxin [HRP]
N/A CCL11/Eotaxin [HRP]
(57 Publications)
Species: Mu
Applications: ELISA
CD46 Antibody
[Unconjugated]
AF2005
Immunohistochemistry CD46 Antibody [Unconjugated]
Western Blot CD46 Antibody [Unconjugated]
(5 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, WB
CapG Antibody
NBP1-90214
Immunocytochemistry/ Immunofluorescence CapG Antibody
Western Blot CapG Antibody
Immunohistochemistry-Paraffin CapG Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
CXCL10/IP-10/CRG-2
[HRP]
DIP100
N/A CXCL10/IP-10/CRG-2 [HRP]
N/A CXCL10/IP-10/CRG-2 [HRP]
N/A CXCL10/IP-10/CRG-2 [HRP]
(151 Publications)
Species: Hu
Applications: ELISA
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(803 Publications)
Species: Hu
Applications: BA
CCR2 Antibody
(48607) [Unconjugated
MAB150
Immunohistochemistry CCR2 Antibody (48607) [Unconjugated]
Flow Cytometry CCR2 Antibody (48607) [Unconjugated]
(43 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC
CXCL8/IL-8
Antibody (6217) [Unco ...
MAB208
Neutralization CXCL8/IL-8 Antibody (6217) [Unconjugated]
Immunocytochemistry CXCL8/IL-8 Antibody (6217) [Unconjugated]
Western Blot CXCL8/IL-8 Antibody (6217) [Unconjugated]
(1 Review)
(180 Publications)
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB
CCR5 Antibody
(45531) [Unconjugated
MAB182
Flow Cytometry CCR5 Antibody (45531) [Unconjugated]
Neutralization CCR5 Antibody (45531) [Unconjugated]
Immunocytochemistry CCR5 Antibody (45531) [Unconjugated]
(1 Review)
(32 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, ICC, Neut
CCL3/MIP-1 alpha
[HRP]
MMA00
N/A CCL3/MIP-1 alpha [HRP]
N/A CCL3/MIP-1 alpha [HRP]
(77 Publications)
Species: Mu
Applications: ELISA
CXCL9/MIG Antibody
[Unconjugated]
AF-492-NA
Neutralization CXCL9/MIG Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence CXCL9/MIG Antibody [Unconjugated]
Western Blot CXCL9/MIG Antibody [Unconjugated]
(31 Publications)
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), Neut
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(445 Publications)
Species: Hu
Applications: BA
IL-4 [Unconjugated]
6507-IL/CF
Bioactivity IL-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(287 Publications)
Species: Mu
Applications: ELISA
CXCL1/GRO
alpha/KC/CINC-1 [Biot
DY453
N/A CXCL1/GRO alpha/KC/CINC-1 [Biotin]
(280 Publications)
Species: Mu
Applications: ELISA

Publications for CCL8/MCP-2 (281-CP/CF)(9)

We have publications tested in 2 confirmed species: Human, Bovine.

We have publications tested in 2 applications: Bioassay, ELISA (Standard).


Filter By Application
Bioassay
(7)
ELISA (Standard)
(1)
All Applications
Filter By Species
Human
(7)
Bovine
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using 281-CP/CF Applications Species
Z Su, J Brooks, J Pelker, T Andreyeva, H Sobon, R Gifford, M Powers, J Wang, C Dower, M Hegen, D Messing, AD Sheehan, JJ Brennan Studies with neutralizing antibodies suggest CXCL8-mediated neutrophil activation is independent of C-C motif chemokine receptor-like 2 (CCRL2) ligand binding function PLoS ONE, 2023-01-20;18(1):e0280590. 2023-01-20 [PMID: 36662882] (Bioassay, Human) Bioassay Human
J Lee, Y Lee, KH Kim, DK Kim, KW Joo, SJ Shin, YS Kim, SH Yang Chemokine (C-C Motif) Ligand 8 and Tubulo-Interstitial Injury in Chronic Kidney Disease Cells, 2022-02-14;11(4):. 2022-02-14 [PMID: 35203308] (Bioassay, Human) Bioassay Human
Y Barsheshet, G Wildbaum, E Levy, A Vitenshtei, C Akinseye, J Griggs, SA Lira, N Karin CCR8(+)FOXp3(+) Treg cells as master drivers of immune regulation Proc. Natl. Acad. Sci. U.S.A., 2017-05-22;0(0):. 2017-05-22 [PMID: 28533380] (Bioassay, Human) Bioassay Human
R Sakumoto, KG Hayashi, S Fujii, H Kanahara, M Hosoe, T Furusawa, K Kizaki Possible Roles of CC- and CXC-Chemokines in Regulating Bovine Endometrial Function during Early Pregnancy Int J Mol Sci, 2017-03-31;18(4):. 2017-03-31 [PMID: 28362325] (Bioassay, Bovine) Bioassay Bovine
Karta , Maya R, Wickert , Lisa E, Curran , Colleen, Gavala , Monica L, Denlinger , Loren C, Gern , James E, Bertics , Paul J Allergen challenge in vivo alters rhinovirus-induced chemokine secretion from human airway macrophages. J Allergy Clin Immunol, 2014-02-14;133(4):1227-30. 2014-02-14 [PMID: 24529411] (Human) Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human) Bioassay Human
Nguyen AN, Stebbins EG, Henson M, O&amp;apos;Young G, Choi SJ, Quon D, Damm D, Reddy M, Ma JY, Haghnazari E, Kapoun AM, Medicherla S, Protter A, Schreiner GF, Kurihara N, Anderson J, Roodman GD, Navas TA, Higgins LS Normalizing the bone marrow microenvironment with p38 inhibitor reduces multiple myeloma cell proliferation and adhesion and suppresses osteoclast formation. Exp. Cell Res., 2006-04-04;312(10):1909-23. 2006-04-04 [PMID: 16600214] (Bioassay, Human) Bioassay Human
Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands. J. Immunol., 2004-12-01;173(11):6592-602. 2004-12-01 [PMID: 15557149] (Bioassay, Human) Bioassay Human
Tay SS, McCormack A, Rose ML Effect of cognate human CD4+ T cell and endothelial cell interactions upon chemokine production. Transplantation, 2004-10-15;78(7):987-94. 2004-10-15 [PMID: 15480163] (ELISA (Standard)) ELISA (Standard)

Reviews for CCL8/MCP-2 (281-CP/CF) (0)

There are no reviews for CCL8/MCP-2 (281-CP/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for CCL8/MCP-2 (281-CP/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CCL8/MCP-2 Products

Blogs on CCL8/MCP-2

There are no specific blogs for CCL8/MCP-2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human CCL8/MCP-2 Protein, CF and receive a gift card or discount.


Bioinformatics

Gene Symbol CCL8
Uniprot