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Recombinant Human CCL5/Met-RANTES Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

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Catalog# & Formulation Size Price
Standard Formulation
335-RM-025/CF
Carrier Free
335-RM-025/CF
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Recombinant Human CCL5/Met-RANTES Protein Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR5. The ED50 for this effect is 0.02-0.1 µg/mL. Measured by its ability to chemoattract 2-day cultured human monocytes. Schall, T. et al. (1990) Nature 347:669. The ED50 for this effect is 0.4-1.2 µg/mL.
Source
E. coli-derived human CCL5/RANTES protein
Ser24-Ser91, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
CCL5
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Bioactivity2
Theoretical MW
7.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
335-RM in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL5/Met-RANTES Protein

  • CCL5
  • chemokine (C-C motif) ligand 5
  • D17S136Enormally T-expressed, and presumably secreted
  • EoCP
  • Eosinophil chemotactic cytokine
  • RANTES
  • SISd
  • SIS-delta
  • small inducible cytokine A5 (RANTES)
  • small inducible cytokine subfamily A (Cys-Cys), member 5
  • Small-inducible cytokine A5
  • T cell-specific protein P228
  • T-cell specific protein p288
  • TCP228T-cell-specific protein RANTES

Background

CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), is an 8 kDa beta -chemokine that plays a primary role in the inflammatory immune response by means of its ability to attract and activate leukocytes (1 - 3). Human and mouse RANTES exhibit cross-species activity on human and mouse cells (4). Mature human CCL5 shares 75% - 84% aa sequence identity with canine, cotton rat, feline, mouse, and rat CCL5 (5). CCL5 is secreted by many cell types at inflammatory sites, and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5 (6, 7). Inflammatory responses can be impaired by the sequestration of CCL5 by the cytomegalovirus protein US28 (8). In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1 (9). The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor and more effectively blocks M-tropic HIV-1 infection of monocytes (10). Oligomerization of CCL5 on glycosaminoglycans is required for CCR1-mediated leukocyte adhesion and activation as well as CCL5’s interaction with the chemokine CXCL4/PF4 (11 - 13). The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature, but CCL5 oligomerization is not required for the extravasation of adherent leukocytes (14 - 16). CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of proinflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature (17). The Met-RANTES preparation from R&D Systems has been shown to be a partial agonist in monocyte chemotactic assays, exhibiting an ED50 10 - 20 fold higher than that of recombinant RANTES.

  1. Schall, T.J. et al. (1990) Nature 347:669.
  2. Bacon, K.B. et al. (1995) Science 269:1727.
  3. Fischer, F.R. et al. (2001) J. Immunol. 167:1637.
  4. Schall, T.J. et al. (1992) Eur. J. Immunol. 22:1477.
  5. Schall, T.J. et al. (1988) J. Immunol. 141:1018.
  6. Appay, V. and S.L. Rowland-Jones (2001) Trends Immunol. 22:83.
  7. Levy, J.A. (2009) J. Immunol. 182:3945.
  8. Randolph-Habecker, J.R. et al. (2002) Cytokine 19:37.
  9. DeVico, A.L. and Gallo, R.C. (2004) Nat. Rev. Microbiol. 2:401.
  10. Proost, P. et al. (1998) J. Biol. Chem. 273:7222.
  11. Appay, V. et al. (1999) J. Biol. Chem. 274:27505.
  12. Proudfoot, A.E.I. et al. (2003) Proc. Natl. Acad. Sci. 100:1885.
  13. von Hundelshausen, P. et al. (2005) Blood 105:924.
  14. von Hundelshausen, P. et al. (2001) Circulation 103:1772.
  15. Zernecke, A. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1897.
  16. Baltus, T. et al. (2003) Blood 102:1985.
  17. Soria, G. and A. Ben-Baruch (2008) Cancer Lett. 267:271.

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Publications for CCL5/RANTES (335-RM)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Bioassay, In Vivo.


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Bioassay
(2)
In Vivo
(4)
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(2)
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(5)
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Showing Publications 1 - 7 of 7.
Publications using 335-RM Applications Species
T Arsiwala, AS Vogt, AE Barton, V Manolova, F Funk, B Flühmann, MF Bachmann Kupffer Cells and Blood Monocytes Orchestrate the Clearance of Iron-Carbohydrate Nanoparticles from Serum International Journal of Molecular Sciences, 2022-02-28;23(5):. 2022-02-28 [PMID: 35269805] (In Vivo, Mouse) In Vivo Mouse
X Sun, Z Chen Cancer-associated fibroblast-derived CCL5 contributes to cisplatin resistance in A549 NSCLC cells partially through upregulation of lncRNA HOTAIR expression Oncology Letters, 2021-08-02;22(4):696. 2021-08-02 [PMID: 34457051] (Bioassay, Human) Bioassay Human
Y Xu, Y Sun, H Shen, Y Dai, H Liu, R Li, H Zhang, L Wu, X Zhu, X Liu Regulation of the terminal maturation of iNKT cells by mediator complex subunit 23 Nat Commun, 2018-09-24;9(1):3875. 2018-09-24 [PMID: 30250136] (In Vivo, Mouse) In Vivo Mouse
SK Hemalatha, SK Sengodan, R Nadhan, J Dev, RR Sushama, V Somasundar, R Thankappan, A Rajan, NR Latha, GR Varghese, AP Mathew, T Somanathan, P Srinivas Brcal Defective Breast Cancer Cells Induce in vitro Transformation of Cancer Associated Fibroblasts (CAFs) to Metastasis Associated Fibroblasts (MAF) Sci Rep, 2018-09-17;8(1):13903. 2018-09-17 [PMID: 30224826] (Bioassay, Human) Bioassay Human
CCL5/RANTES contributes to hypothalamic insulin signaling for systemic insulin responsiveness through CCR5 Sci Rep, 2016-11-29;6(0):37659. 2016-11-29 [PMID: 27898058] (In Vivo, Mouse) In Vivo Mouse
Kanno S, Nishio H, Tanaka T, Motomura Y, Murata K, Ihara K, Onimaru M, Yamasaki S, Kono H, Sueishi K, Hara T Activation of an innate immune receptor, Nod1, accelerates atherogenesis in Apoe-/- mice. J Immunol, 2014-12-08;194(2):773-80. 2014-12-08 [PMID: 25488987] (Mouse) Mouse
Liou J, Mao C, Ching-Wah Sum D, Liu F, Lai Y, Li J, Day Y Peritoneal Administration of Met-RANTES Attenuates Inflammatory and Nociceptive Responses in a Murine Neuropathic Pain Model. J Pain, 2012-11-23;14(1):24-35. 2012-11-23 [PMID: 23183003] (In Vivo, Mouse) In Vivo Mouse

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Bioinformatics

Gene Symbol CCL5
Uniprot