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Recombinant Equine IL-1 beta/IL-1F2 Protein, CF

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Product Details

Summary
Reactivity EqSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
3340-EL-010
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Recombinant Equine IL-1 beta/IL-1F2 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 5-30 pg/mL.
Source
E. coli-derived equine IL-1 beta/IL-1F2 protein
Ala116-Ala268 (Glu179Gly, Met188Thr, Thr194Ile, Ser245Lys and Arg256Gln)
Accession #
N-terminal Sequence
Ala116
Protein/Peptide Type
Recombinant Proteins
Gene
IL1B
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
3340-EL/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Equine IL-1 beta/IL-1F2 Protein, CF

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in equine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1 - 4). The equine IL-1 beta cDNA encodes a 268 aa precursor. A 115 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5 - 7). An alternatively spliced form of equine IL-1 beta has a deletion which encompasses the Caspase-1 cleavage site and potentially results in a membrane-associated form (8). The 17 kDa mature equine IL-1 beta shares 65% - 75% aa sequence identity with canine, cotton rat, feline, human, mouse, porcine, rat, and rhesus IL-1 beta .

  1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
  4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
  5. Kato, H. et al. (1997) Vet. Immunol. Immunopathol. 48:221.
  6. Howard, R.D. et al. (1998) Am. J. Vet. Res. 59:704.
  7. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
  8. Kato, H. et al. (1996) Gene 177:11.

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3340-EL/CF
Recombinant Equine IL-1 beta/IL-1F2 Protein, CF
Species: Eq
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Publications for IL-1 beta/IL-1F2 (3340-EL/CF)(11)

We have publications tested in 3 confirmed species: Equine, Horse, Porcine.

We have publications tested in 3 applications: Bioassay, Cell Culture, In Vivo.


Filter By Application
Bioassay
(9)
Cell Culture
(1)
In Vivo
(1)
All Applications
Filter By Species
Equine
(9)
Horse
(1)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using 3340-EL/CF Applications Species
NA Moyo, D Westcott, R Simmonds, F Steinbach Equine Arteritis Virus in Monocytic Cells Suppresses Differentiation and Function of Dendritic Cells Viruses, 2023-01-16;15(1):. 2023-01-16 [PMID: 36680295] (Bioassay, Equine) Bioassay Equine
LC Garbin, CW McIlwraith, DD Frisbie Use of allogeneic freeze-dried conditioned serum for the prevention of degradation in cartilage exposed to IL-1� Oncogene, 2022-07-11;18(1):265. 2022-07-11 [PMID: 35820849] (Bioassay, Equine) Bioassay Equine
B Jeremiasse, C Matta, CR Fellows, DJ Boocock, JR Smith, S Liddell, F Lafeber, WE van Spil, A Mobasheri Alterations in the chondrocyte surfaceome in response to pro-inflammatory cytokines BMC Mol Cell Biol, 2020-06-26;21(1):47. 2020-06-26 [PMID: 32586320] (Bioassay, Equine) Bioassay Equine
L Bundgaard, A Stensballe, KJ Elbæk, LC Berg Mass spectrometric analysis of the in vitro secretome from equine bone marrow-derived mesenchymal stromal cells to assess the effect of chondrogenic differentiation on response to interleukin-1&amp;beta treatment Stem Cell Res Ther, 2020-05-20;11(1):187. 2020-05-20 [PMID: 32434555] (Cell Culture, Horse) Cell Culture Horse
L McGlinchey, G Agne, T Passler, R Cole, J Schumacher An Objective Assessment of the Effect of Anesthetizing the Median Nerve on Lameness Caused by Pain in the Cubital Joint J. Equine Vet. Sci., 2018-12-19;75(0):9-13. 2018-12-19 [PMID: 31002100] (In Vivo, Equine) In Vivo Equine
JM Cassano, LV Schnabel, MB Goodale, LA Fortier Inflammatory licensed equine MSCs are chondroprotective and exhibit enhanced immunomodulation in an inflammatory environment Stem Cell Res Ther, 2018-04-03;9(1):82. 2018-04-03 [PMID: 29615127] (Bioassay, Equine) Bioassay Equine
M Castro Mar, MJ Peffers, K Lee, LM Rubio-Mart Effects of stanozolol on normal and IL-1?-stimulated equine chondrocytes in vitro BMC Vet. Res., 2018-03-20;14(1):103. 2018-03-20 [PMID: 29554899] (Bioassay, Equine) Bioassay Equine
H Singleton, SP Graham, JP Frossard, KB Bodman-Smi, F Steinbach Infection of monocytes with European porcine reproductive and respiratory syndrome virus (PRRSV-1) strain Lena is significantly enhanced by dexamethasone and IL-10 Virology, 2018-03-02;0(0):. 2018-03-02 [PMID: 29502802] (Bioassay, Porcine) Bioassay Porcine
LJ Berghaus, S Giguère, AI Bordin, ND Cohen Effects of priming with cytokines on intracellular survival and replication of Rhodococcus equi in equine macrophages Cytokine, 2017-12-12;102(0):7-11. 2017-12-12 [PMID: 29245049] (Bioassay, Equine) Bioassay Equine
Danièle Noel Utility of a Mouse Model of Osteoarthritis to Demonstrate Cartilage Protection by IFN?-Primed Equine Mesenchymal Stem Cells Front Immunol, 2016-09-27;7(0):392. 2016-09-27 [PMID: 27729913] (Bioassay, Equine) Bioassay Equine
Show All 11 Publications.

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Bioinformatics

Gene Symbol IL1B
Uniprot