![Western Blot: RBM27 Antibody [NB100-60426] - Detection of Human and Mouse RBM27 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-RBM27 antibody NB100-60426 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RBM27-Antibody-Western-Blot-NB100-60426-img0012.jpg "Western Blot: RBM27 Antibody [NB100-60426] - Detection of Human and Mouse RBM27 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-RBM27 antibody NB100-60426 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 700 and 750 of human RNA binding motif protein 27 using the numbering given in entry Q9P2N5.2 (GeneID 54439). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RBM27 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry: RBM27 Antibody [NB100-60426] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- RBM27 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/RBM27-Antibody-Immunohistochemistry-NB100-60426-img0009.jpg "Immunohistochemistry: RBM27 Antibody [NB100-60426] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- RBM27 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![Western Blot: RBM27 Antibody [NB100-60426] - Detection of Human RBM27 on HeLa whole cell lysate using NB100-60426. RBM27 was also immunoprecipitated by rabbit anti-RBM27 antibody NB100-60427.](http://images.novusbio.com/fullsize/RBM27-Antibody-Western-Blot-NB100-60426-img0003.jpg "Western Blot: RBM27 Antibody [NB100-60426] - Detection of Human RBM27 on HeLa whole cell lysate using NB100-60426. RBM27 was also immunoprecipitated by rabbit anti-RBM27 antibody NB100-60427.")
![Immunoprecipitation: RBM27 Antibody [NB100-60426] - Detection of human RBM27 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RBM27 antibody NB100-60426 used for IP at 6 ug per reaction. RBM27 was also immunoprecipitated by another rabbit anti-RBM27 antibody. For blotting immunoprecipitated RBM27, NB100-60426 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/RBM27-Antibody-Immunoprecipitation-NB100-60426-img0013.jpg "Immunoprecipitation: RBM27 Antibody [NB100-60426] - Detection of human RBM27 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RBM27 antibody NB100-60426 used for IP at 6 ug per reaction. RBM27 was also immunoprecipitated by another rabbit anti-RBM27 antibody. For blotting immunoprecipitated RBM27, NB100-60426 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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