Pyruvate Carboxylase Antibody - BSA Free

Pyruvate Carboxylase (PC) catalyzes an important 2-step reaction - (i) ATP-dependent carboxylation of covalently attached biotin (ii) transfer of carboxyl group to pyruvate, and this interconversion is essential for replenishment of TCA/citric acid cycle intermediates (anaplerosis). By extension, PC participates in many pathways that depend on oxaloacetate (OAA) such as gluconeogenesis, glycogen synthesis, lipogenesis, glycerogenesis, glucose-dependent insulin secretion, and synthesis of amino acids as well as neurotransmitters. With highest expression levels in liver, adipose tissue, kidney, lactating mammary gland, pancreatic islets etc, PC localize to cellular mitochondrial matrix and its activity is highly regulated by its allosteric activator acetyl-CoA. Enhanced acetyl-CoA levels indicate a need for increased OAA synthesis to replenish TCA cycle intermediates or a diversion of pyruvate for glucose synthesis in gluconeogenic tissues. PC's deficiency causes a fatal multiorgan metabolic imbalance that predominantly manifests with lactic acidemia and neurological dysfunction at an early age. Three clinical forms of PC deficiency include an infantile or mild form (Type A), a severe neonatal form (Type B), and a benign form (Type C), all of which exhibit clinical or biochemical parallals of impaired anaplerosis.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: IF/IHC, WB.

Showing Publications 1 - 10 of 15. Show All 15 Publications. Collapse Publications.

Publications using NBP1-49536	Applications	Species
Shankar TS, Ramadurai DKA, Steinhorst K et al. Cardiac-specific deletion of voltage dependent anion channel 2 leads to dilated cardiomyopathy by altering calcium homeostasis Nature Communications 2021-07-28 [PMID: 34321484] (WB)	WB
Rattanapornsompong K, Ngamkham J, Chavalit T, Jitrapakdee S. Generation of Human Pyruvate Carboxylase Knockout Cell Lines Using Retrovirus Expressing Short Hairpin RNA and CRISPR-Cas9 as Models to Study Its Metabolic Role in Cancer Research Methods Mol. Biol. 2019-12-12 [PMID: 30535704] (IF/IHC, Human)	IF/IHC	Human
Toledo M, Batista-Gonzalez A, Merheb E et al. Autophagy Regulates the Liver Clock and Glucose Metabolism by Degrading CRY2 Cell Metab. 2018-08-07 [PMID: 29937374] (Mouse)		Mouse
Li X, Han G, Li X et al. Mitochondrial pyruvate carrier function determines cell stemness and metabolic reprogramming in cancer cells Oncotarget 2017-07-11 [PMID: 28624784] (WB, Mouse)	WB	Mouse
Seale LA, Gilman CL, Hashimoto AC et al. Diet-induced obesity in the selenocysteine lyase knockout mouse Antioxid. Redox Signal. 2015-07-20 [PMID: 26192035]
Tao H, Zhang Y, Zeng X et al. Niclosamide ethanolamine-induced mild mitochondrial uncoupling improves diabetic symptoms in mice. Nat Med 2014-11-01 [PMID: 25282357] (WB)	WB
Lee JH, Jung IR, Choi SE et al. Toxicity generated through inhibition of pyruvate carboxylase and carnitine palmitoyl transferase-1 is similar to high glucose/palmitate-induced glucolipotoxicity. Mol Cell Endocrinol 2013-12-11 [PMID: 24333689] (WB, Rat)	WB	Rat
Lee W, Reyes RC, Gottipati MK et al. Enhanced Ca2+-dependent glutamate release from astrocytes of the BACHD Huntington's disease mouse model. Neurobiol Dis 2013-06-10 [PMID: 23756199] (WB, Mouse)	WB	Mouse
Vastag L, Koyuncu E, Grady SL et al. Divergent effects of human cytomegalovirus and herpes simplex virus-1 on cellular metabolism. PLoS Pathog. 2011-07-01 [PMID: 21779165] (WB, Human)	WB	Human
Chung C, Singh K, Kotiadis V et al. Maladaptive nutrient signalling sustains the m.3243A>G mtDNA mutation bioRxiv 2020-06-19 (WB, Human)	WB	Human
Song X, Liu J, Kuang F et al. PDK4 dictates metabolic resistance to ferroptosis by suppressing pyruvate oxidation and fatty acid synthesis Cell reports 2021-02-23 [PMID: 33626342]
Guo X, Jiang X, Chen K et al. The Role of Palmitoleic Acid in Regulating Hepatic Gluconeogenesis through SIRT3 in Obese Mice Nutrients 2022-04-01 [PMID: 35406095] (WB, Mouse)	WB	Mouse
Schworer S, Pavlova NN, Cimino FV Et al. Fibroblast pyruvate carboxylase is required for collagen production in the tumour microenvironment Nature metabolism 2021-11-01 [PMID: 34764457] (WB, Human)	WB	Human
Chung CY, Singh K, Kotiadis VN Et al. Constitutive activation of the PI3K-Akt-mTORC1 pathway sustains the G mtDNA mutation Nature communications 2021-11-04 [PMID: 34737295] (WB, Human)	WB	Human
Show All 15 Publications. Collapse Publications.

Array NBP1-49536

• Pyruvate Carboxylase Antibodies
• Pyruvate Carboxylase Lysates
• Pyruvate Carboxylase Proteins