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Rad51L1 Antibody (OTI7H4)

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Western Blot: Rad51L1 Antibody (7H4) [NBP2-01220] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Rad51L1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Rad51L1.
Western Blot: Rad51L1 Antibody (7H4) [NBP2-01220] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Rad51L1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunocytochemistry/ Immunofluorescence: Rad51L1 Antibody (7H4) [NBP2-01220] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Rad51L1.
Flow Cytometry: Rad51L1 Antibody (7H4) [NBP2-01220] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Rad51L1 antibody, and then ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
OTI7H4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.27 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-73782AF350
Alexa Fluor 405
NBP2-73782AF405
Alexa Fluor 488
NBP2-73782AF488
Alexa Fluor 532
NBP2-73782AF532
Alexa Fluor 594
NBP2-73782AF594
Alexa Fluor 647
NBP2-73782AF647
Alexa Fluor 700
NBP2-73782AF700
Alexa Fluor 750
NBP2-73782AF750
Allophycocyanin
NBP2-73782APC
Allophycocyanin/Cy7
NBP2-73782APCCY7
Biotin
NBP2-73782B
CoraFluor 1
NBP2-73782CL1
DyLight 350
NBP2-73782UV
DyLight 405
NBP2-73782V
DyLight 488
NBP2-73782G
DyLight 550
NBP2-73782R
DyLight 594
NBP2-73782DL594
DyLight 650
NBP2-73782C
DyLight 680
NBP2-73782FR
DyLight 755
NBP2-73782IR
FITC
NBP2-73782F
HRP
NBP2-73782H
Janelia Fluor 525
NBP2-73782JF525
Janelia Fluor 549
NBP2-73782JF549
Janelia Fluor 585
NBP2-73782JF585
Janelia Fluor 635
NBP2-73782JF635
Janelia Fluor 646
NBP2-73782JF646
Janelia Fluor 669
NBP2-73782JF669
PE
NBP2-73782PE
PE/Atto594
NBP2-73782PEATT594
PE/Cy5.5
NBP2-73782PECY55
PE/Cy7
NBP2-73782PECY7
PerCP
NBP2-73782PCP
mFluor Violet 450 SE
NBP2-73782MFV450
mFluor Violet 500 SE
NBP2-73782MFV500
mFluor Violet 610 SE
NBP2-73782MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-73782
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Rad51L1 Antibody (OTI7H4) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 119-346 of human RAD51L1(NP_598193) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RAD51B
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.27 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Rad51L1 Antibody (OTI7H4)

  • DNA repair protein RAD51 homolog 2
  • MGC34245
  • R51H2REC2hREC2
  • RAD51 (S. cerevisiae)-like 1
  • RAD51 homolog B
  • RAD51B
  • RAD51-like 1 (S. cerevisiae)
  • RAD51-like protein 1
  • RecA-like protein
  • recombination repair protein

Background

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are evolutionarily conserved proteins essential for DNA repair by homologous recombination. This protein has been shown to form a stable heterodimer with the family member RAD51C, which further interacts with the other family members, such as RAD51, XRCC2, and XRCC3. Overexpression of this gene was found to cause cell cycle G1 delay and cell apoptosis, which suggested a role of this protein in sensing DNA damage. At least three alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RAD51B
Entrez