![Western Blot: HID1 Antibody (OTI2F4) [NBP2-02667] - Analysis of extracts (10ug) from 10 Human tissue by using anti-C17orf28 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).](http://images.novusbio.com/fullsize/HID1-Antibody-OTI2F4-Western-Blot-NBP2-02667-img0015.jpg "Western Blot: HID1 Antibody (OTI2F4) [NBP2-02667] - Analysis of extracts (10ug) from 10 Human tissue by using anti-C17orf28 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).")
| Reactivity | Hu, Mu, Rt, Ca, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI2F4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length human recombinant protein of human C17orf28 (NP_085133) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | HID1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 88.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
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![Immunohistochemistry-Paraffin Rad17 [p Ser645] Antibody](https://images.novusbio.com/images/Rad17-[p-Ser645]-Antibody-Immunohistochemistry-Paraffin-NBP2-24457-img0003.jpg)
![Western Blot Rad17 [p Ser645] Antibody](https://images.novusbio.com/images/Rad17-[p-Ser645]-Antibody-Western-Blot-NBP2-24457-img0005.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: HID1 Antibody (2F4) [NBP2-02667] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY C17orf28.](http://images.novusbio.com/fullsize/HID1-Antibody-2F4-Immunocytochemistry-Immunofluorescence-NBP2-02667-img0010.jpg "Immunocytochemistry/Immunofluorescence: HID1 Antibody (2F4) [NBP2-02667] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY C17orf28.")
![Immunohistochemistry-Paraffin: HID1 Antibody (2F4) [NBP2-02667] - Staining of paraffin-embedded Human prostate tissue using anti-C17orf28 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HID1-Antibody-2F4-Immunohistochemistry-Paraffin-NBP2-02667-img0012.jpg "Immunohistochemistry-Paraffin: HID1 Antibody (2F4) [NBP2-02667] - Staining of paraffin-embedded Human prostate tissue using anti-C17orf28 mouse monoclonal antibody.")
![Flow Cytometry: HID1 Antibody (2F4) [NBP2-02667] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-C17orf28 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/HID1-Antibody-2F4-Flow-Cytometry-NBP2-02667-img0009.jpg "Flow Cytometry: HID1 Antibody (2F4) [NBP2-02667] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-C17orf28 antibody, and then analyzed by flow cytometry.")
![Western Blot: HID1 Antibody (2F4) [NBP2-02667] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C17orf28 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C17orf28.](http://images.novusbio.com/fullsize/HID1-Antibody-2F4-Western-Blot-NBP2-02667-img0013.jpg "Western Blot: HID1 Antibody (2F4) [NBP2-02667] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C17orf28 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C17orf28.")
![Western Blot: HID1 Antibody (2F4) [NBP2-02667] - Analysis of extracts (35ug) from 9 different cell lines by using anti-C17orf28 monoclonal antibody.](http://images.novusbio.com/fullsize/HID1-Antibody-2F4-Western-Blot-NBP2-02667-img0014.jpg "Western Blot: HID1 Antibody (2F4) [NBP2-02667] - Analysis of extracts (35ug) from 9 different cell lines by using anti-C17orf28 monoclonal antibody.")
![Immunohistochemistry-Paraffin: HID1 Antibody (2F4) [NBP2-02667] - Staining of paraffin-embedded Human liver tissue using anti-C17orf28 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HID1-Antibody-2F4-Immunohistochemistry-Paraffin-NBP2-02667-img0011.jpg "Immunohistochemistry-Paraffin: HID1 Antibody (2F4) [NBP2-02667] - Staining of paraffin-embedded Human liver tissue using anti-C17orf28 mouse monoclonal antibody.")
![Flow Cytometry: HID1 Antibody (2F4) [NBP2-02667] - Analysis of Jurkat cells, using anti-C17orf28 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/HID1-Antibody-2F4-Flow-Cytometry-NBP2-02667-img0008.jpg "Flow Cytometry: HID1 Antibody (2F4) [NBP2-02667] - Analysis of Jurkat cells, using anti-C17orf28 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")