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HMGA2 Antibody [Unconjugated]

 (11 Publications)
 
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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, MDA-MD-231 human breast cancer cell line, NIH-3T3 mouse embryonic fibroblast cell line, and P19 mouse embryonal carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse HMGA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3184) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HMGA2 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, MDA-MD-231 human breast cancer cell line, NIH-3T3 mouse embryonic fibroblast cell line, and P19 mouse embryonal carcinoma cell line. PVDF ...read more
HMGA2 was detected in immersion fixed IMR‑90 human lung fibroblast cell line using Goat Anti-Human/Mouse HMGA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3184) at 5 µg/mL for 3 hours at ...read more
Simple Western lane view shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HMGA2 at approximately 30 kDa (as indicated) using 10 µg/mL of Goat ...read more
Immunocytochemical staining.A: Native CT1258 cells, B: CT1258-EGFP cells, C: CT1258-EGFP-HMGA2 cells. Approximately 50% of the native CT1258 cell line and of CT1258-EGFP cells showed a HMGA2-positive nuclear labelling. ...read more
Nuclear Src activation and association with HMGA2 and SMYD3 gene promoters are observed in patient samples of PDACA Immunohistochemistry of human PDAC tumor samples. Human PDAC slides were immunostained for pY416Src (i) ...read more
Nuclear Src and p300 associate with HMGA2 and SMYD3 gene promoters and regulate their expression in PDAC and MEF cellsA and B. Chromatin-immunoprecipitation (ChIP) and quantitative polymerase-chain reaction (qPCR) ...read more
Immunocytochemical staining.A: Native CT1258 cells, B: CT1258-EGFP cells, C: CT1258-EGFP-HMGA2 cells. Approximately 50% of the native CT1258 cell line and of CT1258-EGFP cells showed a HMGA2-positive nuclear labelling. ...read more
HMGA2 immunohistochemistry in canine OSCC. Immunolabelling of a canine tumour grade II: overview (A), tumour centre (B) and invasive front (C). HMGA2 staining in the tumour centre (B) revealed approx. 25% tumour cells ...read more
HMGA2 immunohistochemistry in human OSCC. Immunolabelling of a human tumour: overview (A), tumour centre (B) and invasive front (C). In the tumour centre (B) lower numbers of tumour cells with nuclear immunolabelling ...read more
HMGA2 immunohistochemistry in canine OSCC. Immunolabelling of a canine tumour grade II: overview (A), tumour centre (B) and invasive front (C). HMGA2 staining in the tumour centre (B) revealed approx. 25% tumour cells ...read more
HMGA2 immunohistochemistry in human OSCC. Immunolabelling of a human tumour: overview (A), tumour centre (B) and invasive front (C). In the tumour centre (B) lower numbers of tumour cells with nuclear immunolabelling ...read more
HMGA2 immunohistochemistry in canine OSCC. Immunolabelling of a canine tumour grade II: overview (A), tumour centre (B) and invasive front (C). HMGA2 staining in the tumour centre (B) revealed approx. 25% tumour cells ...read more
HMGA2 immunohistochemistry in human OSCC. Immunolabelling of a human tumour: overview (A), tumour centre (B) and invasive front (C). In the tumour centre (B) lower numbers of tumour cells with nuclear immunolabelling ...read more
Immunocytochemical staining.A: Native CT1258 cells, B: CT1258-EGFP cells, C: CT1258-EGFP-HMGA2 cells. Approximately 50% of the native CT1258 cell line and of CT1258-EGFP cells showed a HMGA2-positive nuclear labelling. ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF3184-SP
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HMGA2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human HMGA2
Ser2-Asp109
Accession # P52926
Specificity
Detects human and mouse HMGA2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human HMGB1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
HMGA2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF3184 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HMGA2 Antibody [Unconjugated]

  • BABL
  • BABLHMGI-C
  • high mobility group AT-hook 2
  • High mobility group AT-hook protein 2
  • high mobility group protein HMGI-C
  • high-mobility group (nonhistone chromosomal) protein isoform I-C
  • High-mobility group protein HMGI-C
  • HMGA2
  • HMGIC
  • HMGICSTQTL9
  • LIPO

Background

HMGA2, previously known as HMGI-C, belongs to the group of high mobility chromosomal proteins that have the AT-hook DNA-binding motif. It is highly and ubiquitously expressed during embryogenesis. Chromosomal rearrangements of the HMGA2 gene and overexpression are frequently associated with benign and malignant tumors, respectively. Human and mouse HMGA2 share 95% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HMGA2 Antibody (AF3184)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Canine.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, Western Blot.


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ICC/IF
(2)
IHC
(2)
IHC-P
(4)
Western Blot
(1)
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Human
(6)
Mouse
(2)
Canine
(1)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF3184 Applications Species
T Campbell, O Hawsawi, V Henderson, P Dike, BJ Hwang, Y Liadi, EZ White, J Zou, G Wang, Q Zhang, N Bowen, D Scott, CV Hinton, V Odero-Mara Novel roles for HMGA2 isoforms in regulating oxidative stress and sensitizing to RSL3-Induced ferroptosis in prostate cancer cells Heliyon, 2023-04-07;9(4):e14810. 2023-04-07 [PMID: 37113783] (ICC/IF, Human) ICC/IF Human
T Campbell, O Hawsawi, V Henderson, P Dike, BJ Hwang, Y Liadi, EZ White, J Zou, G Wang, Q Zhang, N Bowen, D Scott, CV Hinton, V Odero-Mara Novel roles for HMGA2 isoforms in regulating oxidative stress and sensitizing to RSL3-Induced ferroptosis in prostate cancer cells Heliyon, 2023;9(4):e14810. 2023 [PMID: 37113783] (ICC/IF, Human) ICC/IF Human
DL Coutu, KD Kokkaliari, L Kunz, T Schroeder Multicolor quantitative confocal imaging cytometry Nat. Methods, 2017-11-13;15(1):39-46. 2017-11-13 [PMID: 29320487] (IHC, Mouse) IHC Mouse
DF Colombo, L Burger, T Baubec, D Schübeler Binding of high mobility group A proteins to the mammalian genome occurs as a function of AT-content PLoS Genet., 2017-12-21;13(12):e1007102. 12/21/2017 [PMID: 29267285]
Paladino David, Yue Peibin, Furuya Hideki et al. A novel nuclear Src and p300 signaling axis controls migratory and invasive behavior in pancreatic cancer. Oncotarget 1/1/2016 [PMID: 26695438]
Sterenczak K, Eckardt A, Kampmann A, Willenbrock S, Eberle N, Langer F, Kleinschmidt S, Hewicker-Trautwein M, Kreipe H, Nolte I, Murua Escobar H, Gellrich N HMGA1 and HMGA2 expression and comparative analyses of HMGA2, Lin28 and let-7 miRNAs in oral squamous cell carcinoma. BMC Cancer, 2014-09-23;14(0):694. 2014-09-23 [PMID: 25245141] (IHC, Human) IHC Human
Willenbrock , Saskia, Wagner , Siegfrie, Reimann-Berg , Nicola, Moulay , Mohammed, Hewicker-Trautwein , Marion, Nolte , Ingo, Murua Escobar , Hugo Generation and characterisation of a canine EGFP-HMGA2 prostate cancer in vitro model. PLoS ONE, 2014-06-10;9(6):e98788. 2014-06-10 [PMID: 24914948] (IHC-P, Canine) IHC-P Canine
Boudoukha S, Rivera Vargas T, Dang I, Kropp J, Cuvellier S, Gautreau A, Polesskaya A MiRNA let-7g regulates skeletal myoblast motility via Pinch-2. FEBS Lett, 2014-03-05;588(9):1623-9. 2014-03-05 [PMID: 24613920] (Western Blot, Mouse) Western Blot Mouse
Downregulation of HMGA2 by the pan-deacetylase inhibitor panobinostat is dependent on hsa-let-7b expression in liver cancer cell lines. Exp Cell Res, 2012-06-08;318(15):1832-43. 2012-06-08 [PMID: 22683924] (IHC-P, Human) IHC-P Human
Sredni ST, Gadd S, Huang CC, Breslow N, Grundy P, Green DM, Dome JS, Shamberger RC, Beckwith JB, Perlman EJ Subsets of very low risk wilms tumor show distinctive gene expression, histologic, and clinical features. Clin. Cancer Res., 2009-11-10;15(22):6800-9. 2009-11-10 [PMID: 19903788] (IHC-P, Human) IHC-P Human
Show All 11 Publications.

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Bioinformatics

Gene Symbol HMGA2
Uniprot