![Western Blot: Rad50 Antibody [NBP2-20055] - Rad50 antibody immunoprecipitates Rad50 protein in IP experiments. IP samples: NT2D1 whole cell extract. A. 50 ug NT2D1 whole cell extract B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of Rad50 protein by 4 ug Rad50 antibody. 5 % SDS-PAGE. The immunoprecipitated Rad50 protein was detected by Rad50 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Rad50-Antibody-Western-Blot-NBP2-20055-img0007.jpg "Western Blot: Rad50 Antibody [NBP2-20055] - Rad50 antibody immunoprecipitates Rad50 protein in IP experiments. IP samples: NT2D1 whole cell extract. A. 50 ug NT2D1 whole cell extract B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of Rad50 protein by 4 ug Rad50 antibody. 5 % SDS-PAGE. The immunoprecipitated Rad50 protein was detected by Rad50 antibody diluted at 1:500.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC, IP, WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human Rad50. The exact sequence is proprietary. |
| Localization | Nucleus; Telomere |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RAD50 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 154 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Rad50 Antibody (NBP2-20055)Find related products by research area.
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RAD50 and DNA Damage Response DNA repair protein RAD50 is a component of the MRN complex (Mre11-RAD50-Nbs1) responsible for DNA double strand break (DSB) repair. DSBs are caused by ionizing radiation, certain chemotherapy drugs, metabolic reactive oxygen species (ROS), replication... Read full blog post. |
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The MRE11 Complex and DNA Damage Response The maintenance of genome stability depends on the DNA damage response (DDR) which is a complex signaling network including cell cycle checkpoints, DNA repair and damage tolerance pathways. The DDR complex has the ability to sense DNA damage and tra... Read full blog post. |
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![Immunohistochemistry-Paraffin: RAD50 Antibody [NBP2-20055] - Immunohistochemical analysis of paraffin-embedded Lung carcinoma, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/RAD50-Antibody-Immunohistochemistry-Paraffin-NBP2-20055-img0003.jpg "Immunohistochemistry-Paraffin: RAD50 Antibody [NBP2-20055] - Immunohistochemical analysis of paraffin-embedded Lung carcinoma, using antibody at 1:250 dilution.")
![Western Blot: RAD50 Antibody [NBP2-20055] - Rad50 antibody [N1N2], N-term detects RAD50 protein by Western blot analysis. A. 30 ug Neuro2A whole lysate/extract B. 30 ug GL261 whole cell lysate/extract5 % SDS-PAGERad50 antibody [N1N2], N-term dilution: 1:1000.](http://images.novusbio.com/fullsize/RAD50-Antibody-Western-Blot-NBP2-20055-img0001.jpg "Western Blot: RAD50 Antibody [NBP2-20055] - Rad50 antibody [N1N2], N-term detects RAD50 protein by Western blot analysis. A. 30 ug Neuro2A whole lysate/extract B. 30 ug GL261 whole cell lysate/extract5 % SDS-PAGERad50 antibody [N1N2], N-term dilution: 1:1000.")
![Western Blot: RAD50 Antibody [NBP2-20055] - Sample (30 ug of whole cell lysate) A: NT2D1 5% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/RAD50-Antibody-Western-Blot-NBP2-20055-img0002.jpg "Western Blot: RAD50 Antibody [NBP2-20055] - Sample (30 ug of whole cell lysate) A: NT2D1 5% SDS PAGE gel, diluted at 1:5000.")
![Western Blot: Rad50 Antibody [NBP2-20055] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Rad50-Antibody-Western-Blot-NBP2-20055-img0004.jpg "Western Blot: Rad50 Antibody [NBP2-20055] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Rad50 Antibody [NBP2-20055] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Rad50-Antibody-Western-Blot-NBP2-20055-img0005.jpg "Western Blot: Rad50 Antibody [NBP2-20055] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Rad50 Antibody [NBP2-20055] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Rad50-Antibody-Western-Blot-NBP2-20055-img0006.jpg "Western Blot: Rad50 Antibody [NBP2-20055] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")