Rad50 Antibody

RAD50 (DNA repair protein RAD50) is a component of MRN complex (Mre11-Rad50-Nbs1) that plays an important role in detection and signaling of DNA double strand breaks (DSBs) through acting as DSB sensor, co-activator of DSB-induced cell cycle checkpoint signaling, and as repair-effector in two competing DSB repair pathways: homologous recombination (HR) and non-homologous end-joining (NHEJ). MRN complex also associates with telomeres at the ends of linear chromosomes, where it contributes to their maintenance. RAD50 is required to bind DNA ends for holding them in close proximity where it facilitates searches for short or long regions of sequence homology in the recombining DNA templates, and also stimulate the activity of DNA ligases and/or restrict MRE11A's nuclease activity to prevent nucleolytic degradation. DSBs can be caused by ionizing radiation, certain chemotherapy drugs, metabolic ROS, as errors during replication, by programmed enzymatic activities during meiosis/V(D)J recombination etc., and if left unrepaired, DSBs can generate chromosomal translocations, aneuploidy and carcinogenesis. Defects in RAD50 have been linked to Nijmegen breakage syndrome-like disorder (NBSLD).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Hamster.

We have publications tested in 7 applications: Dot Blot, ICC/IF, IP, Immunoprecipitation, In Vivo, WB, Western Blot.

Showing Publications 1 - 10 of 34. Show All 34 Publications. Collapse Publications.

Publications using NB100-154	Applications	Species
Einig E, Jin C, Andrioletti V et al. RNAPII-dependent ATM signaling at collisions with replication forks Nature Communications 2023-08-24 [PMID: 37620345] (Dot Blot)	Dot Blot
Pollina EA, Gilliam DT, Landau AT et al. A NPAS4-NuA4 complex couples synaptic activity to DNA repair Nature 2023-02-01 [PMID: 36792830] (In Vivo, Mouse) Details: CUT&RUN	In Vivo	Mouse
Gnedina O, Morshneva A, Skvortsova E, Igotti M HDAC Inhibitor Sodium Butyrate Attenuates the DNA Repair in Transformed but Not in Normal Fibroblasts International Journal of Molecular Sciences 2022-03-23 [PMID: 35408878] (IP, Mouse)	IP	Mouse
Herok M, Wawrzynow B, Maluszek MJ et al. Chemotherapy of HER2- and MDM2-Enriched Breast Cancer Subtypes Induces Homologous Recombination DNA Repair and Chemoresistance Cancers (Basel) 2021-09-07 [PMID: 34572735] (Immunoprecipitation, Western Blot)	Immunoprecipitation, Western Blot
Yoshimura Y Increased error-free DNA repair gene expression through reprogramming in human iPS cells Regenerative Therapy 2019-06-28 [PMID: 31304203] (WB, Human)	WB	Human
Patel JP, Puck JM, Srinivasan R et al. Nijmegen Breakage Syndrome Detected by Newborn Screening for T Cell Receptor Excision Circles (TRECs) J. Clin. Immunol. 2015-02-13 [PMID: 25677497] (WB, Human)	WB	Human
Kondratova A, Watanabe T, Marotta M et al. Replication fork integrity and intra-S phase checkpoint suppress gene amplification. Nucleic Acids Res 2015-02-11 [PMID: 25672394] (WB, Hamster) Details: Using the DyLight 488 conjugated version of NB100-904, catalog number NB100-904G.	WB	Hamster
Okamoto K, Bartocci C, Ouzounov I et al. A two-step mechanism for TRF2-mediated chromosome-end protection. Nature 2013-02-06 [PMID: 23389450] (WB, IP, Human)	WB, IP	Human
Murad NA, Cullen JK, McKenzie M et al. Mitochondrial dysfunction in a novel form of autosomal recessive ataxia. Mitochondrion 2012-11-01 [PMID: 23178371] (WB, Human)	WB	Human
Lu CS, Truong LN, Aslanian A et al. The RING finger protein RNF8 ubiquitinates Nbs1 to promote DNA double-strand break repair by homologous recombination J Biol Chem 2012-10-31 [PMID: 23115235] (WB, Human)	WB	Human
Buisson R, Masson JY. PALB2 self-interaction controls homologous recombination Nucleic Acids Res 2012-08-31 [PMID: 22941656] (Human)		Human
Thompson R, Montano R, Eastman A et al. The Mre11 nuclease is critical for the sensitivity of cells to Chk1 inhibition. PLoS One 2012-01-01 [PMID: 22937147] (WB, Human)	WB	Human
He J, Shi LZ, Truong LN et al. Rad50 zinc hook is important for the Mre11 complex to bind chromosomal DNA double-stranded breaks and initiate various DNA damage responses. J Biol Chem 2012-09-01 [PMID: 22833675] (WB, Human)	WB	Human
Karen KA, Hoey PJ, Young CSH et al. Temporal regulation of the Mre11-Rad50-Nbs1 complex during adenovirus infection. J Virol;83(9):4565-73. 2009-05-01 [PMID: 19244322]
Bouska A, Lushnikova T, Plaza S et al. Mdm2 promotes genetic instability and transformation independent of p53. Mol Cell Biol;28(15):4862-74. 2008-08-01 [PMID: 18541670]
Dery U, Coulombe Y, Rodrigue A, Stasiak A, Richard S, Masson J-Y. A glycine-arginine domain in control of the human MRE11 DNA repair protein. Mol Cell Biol;28(9):3058-69. 2008-05-01 [PMID: 18285453]
Hartl B, Zeller T, Blanchette P et al. Adenovirus type 5 early region 1B 55-kDa oncoprotein can promote cell transformation by a mechanism independent from blocking p53-activated transcription. Oncogene 2008-06-12 [PMID: 18212738] (ICC/IF, WB, Human)	ICC/IF, WB	Human
Chan, N et al. Chronic hypoxia decreases synthesis of homologous recombination proteins to offset chemoresistance and radioresistance. Cancer Res;68(2):605-14. 2008-01-15 [PMID: 18199558]
Stewart GS, Stankovic T, Byrd PJ et al. RIDDLE immunodeficiency syndrome is linked to defects in 53BP1-mediated DNA damage signaling. Proc Natl Acad Sci U S A 2007-10-01 [PMID: 17940005]
Sedelnikova, OA,et al. DNA double-strand breaks form in bystander cells after microbeam irradiation of three-dimensional human tissue models. Cancer Res;67(9):4295-302. 2007-05-01 [PMID: 17483342]
Rink, L et al. Enhanced phosphorylation of Nbs1, a member of DNA repair/checkpoint complex Mre11-RAD50-Nbs1, can be targeted to increase the efficacy of imatinib mesylate against BCR/ABL-positive leukemia cells. Blood;110(2):651-60. 2007-07-15 [PMID: 17431132] (WB, Human, Mouse)	WB	Human, Mouse
Takemura H, Rao VA, Sordet O et al. Defective Mre11-dependent activation of Chk2 by ataxia telangiectasia mutated in colorectal carcinoma cells in response to replication-dependent DNA double strand breaks. J Biol Chem 2006-10-01 [PMID: 16905549]
Sheen, MR et al. Mre11-Rad50-Nbs1 complex is activated by hypertonicity. Am J Physiol Renal Physiol;291(5):F1014-20. 2006-11-01 [PMID: 16788144]
Kong LJ, Meloni AR, Nevins JR. The Rb-related p130 protein controls telomere lengthening through an interaction with a Rad50-interacting protein, RINT-1. Mol Cell 2006-04-01 [PMID: 16600870]
Cerosaletti, K et al. Active role for nibrin in the kinetics of atm activation. Mol Cell Biol;26(5):1691-9. 2006-03-01 [PMID: 16478990]
Alt, JR et al. Mdm2 binds to Nbs1 at sites of DNA damage and regulates double strand break repair. J Biol Chem;280(19):18771-81. 2005-05-13 [PMID: 15734743] (WB, IP, Human)	WB, IP	Human
Fan, R et al. Defective DNA strand break repair after DNA damage in prostate cancer cells: implications for genetic instability and prostate cancer progression. Cancer Res;64(23):8526-33. 2004-12-01 [PMID: 15574758]
Cerosaletti K, Concannon P. Independent roles for nibrin and Mre11-Rad50 in the activation and function of Atm. J Biol Chem 2004-09-01 [PMID: 15234984]
Cheng, W et al. Linkage between Werner syndrome protein and the Mre11 complex via Nbs1. J Biol Chem;279(20):21169-76. 2004-05-14 [PMID: 15026416] (IP, Human)	IP	Human
Sedelnikova, O et al. Senescing human cells and ageing mice accumulate DNA lesions with unrepairable double-strand breaks. Nat Cell Biol;6(2):168-70. 2004-02-01 [PMID: 14755273] (ICC/IF, Human)	ICC/IF	Human
Udayakumar,D et al. Distinct Pathways of Nonhomologous End Joining That Are Differentially Regulated by DNA-dependent Protein Kinase-mediated Phosphorylation. Journal of Biological Chemistry 278(43):41631-41635. 2003-01-01 [PMID: 12917393]
Siwicki, J et al. Telomere maintenance and cell cycle regulation in spontaneously immortalized T-cell lines from Nijmegen breakage syndrome patients. Exp Cell Res;287(1):178-89. 2003-07-01 [PMID: 12799193]
Furuta, T et al. Phosphorylation of histone H2AX and activation of Mre11, Rad50, and Nbs1 in response to replication-dependent DNA double-strand breaks induced by mammalian DNA topoisomerase I cleavage complexes. J Biol Chem;278(22):20303-12. 2003-05-30 [PMID: 12660252] (ICC/IF, WB, Human)	ICC/IF, WB	Human
Giannini, G et al. Human MRE11 is inactivated in mismatch repair-deficient cancers. EMBO Rep;3(3):248-54. 2002-03-01 [PMID: 11850399]
Show All 34 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Verified Customer	WB	Human	02/18/2019	View Summary Application Western Blot Sample Tested human lung cancer cell line Species Human Lot E1
Enlarge	4 reviewed by: Anatoli Grechov	WB	Human	12/17/2014	View Summary Application Western Blot Sample Tested 293T lysate Species Human

Array NB100-154

• Rad50 Antibodies
• Rad50 ELISA Kits
• Rad50 Protein