Rad50 Antibody

RAD50 (DNA repair protein RAD50) is a component of MRN complex (Mre11-Rad50-Nbs1) that plays an important role in detection and signaling of DNA double strand breaks (DSBs) through acting as DSB sensor, co-activator of DSB-induced cell cycle checkpoint signaling, and as repair-effector in two competing DSB repair pathways: homologous recombination (HR) and non-homologous end-joining (NHEJ). MRN complex also associates with telomeres at the ends of linear chromosomes, where it contributes to their maintenance. RAD50 is required to bind DNA ends for holding them in close proximity where it facilitates searches for short or long regions of sequence homology in the recombining DNA templates, and also stimulate the activity of DNA ligases and/or restrict MRE11A's nuclease activity to prevent nucleolytic degradation. DSBs can be caused by ionizing radiation, certain chemotherapy drugs, metabolic ROS, as errors during replication, by programmed enzymatic activities during meiosis/V(D)J recombination etc., and if left unrepaired, DSBs can generate chromosomal translocations, aneuploidy and carcinogenesis. Defects in RAD50 have been linked to Nijmegen breakage syndrome-like disorder (NBSLD).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.

Showing Publications 1 - 4 of 4.

Publications using NB100-1487	Applications	Species
Zhou H, Kawamura K, Yanagihara H et al. NBS1 is regulated by two kind of mechanisms: ATM-dependent complex formation with MRE11 and RAD50, and cell cycle-dependent degradation of protein J Radiat Res. 2017-03-22 [PMID: 28369484] (WB, Human) Details: The regulatory mechanisms of the MRN complex, formed by NBS1 with MRE11 and RAD50, are investigated through immunoprecipitation.	WB	Human
Chang L, Huang J, Wang K et al. Targeting Rad50 sensitizes human nasopharyngeal carcinoma cells to radiotherapy. BMC Cancer 2016-03-07 [PMID: 26951044] (WB)	WB
Kobayashi J, Saito Y, Okui M, Miwa N. Increased oxidative stress in AOA3 cells disturbs ATM-dependent DNA damage responses Mutation Research/Genetic Toxicology and Environmental Mutagenesis 2015-03-18 [PMID: 25868131] (WB, Human)	WB	Human
Cahill, D et al. Dimerization of the Rad50 protein is independent of the conserved hook domain. Mutagenesis;22(4):269-74. 2007-07-01 [PMID: 17426050] (WB, Human)	WB	Human

Array NB100-1487

• Rad50 Antibodies
• Rad50 ELISA Kits
• Rad50 Protein