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PU.1/Spi-1 Antibody (S02-1D7)

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Western Blot: PU.1/Spi-1 Antibody (S02-1D7) [NBP3-19911] - Western blot detection of PU.1/Spi1 in CHO-K1, C6 cell lysates using NBP3-19911 (1:1000 diluted). Predicted band size: 31kDa. Observed band size: 31kDa.
Western Blot: PU.1/Spi-1 Antibody (S02-1D7) [NBP3-19911] - Western blot detection of PU.1/Spi1 in CHO-K1, C6 cell lysates using NBP3-19911 (1:1000 diluted). Predicted band size: 31kDa. Observed band size: 31kDa.
Immunocytochemistry/ Immunofluorescence: PU.1/Spi-1 Antibody (S02-1D7) [NBP3-19911] - Immunocytochemistry of PU.1/Spi1 (green) in Raw264. 7 using NBP3-19911 at dilution 1/50, and DAPI (blue)

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ICC/IF
Clone
S02-1D7
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.3 mg/ml

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PU.1/Spi-1 Antibody (S02-1D7) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide of mouse PU.1/Spi1 (Uniprot # P17433)
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
SPI1
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1/50
  • Western Blot 1/1000
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Concentration
0.3 mg/ml
Purity
Affinity purified

Alternate Names for PU.1/Spi-1 Antibody (S02-1D7)

  • hematopoietic transcription factor PU.1
  • OF
  • PU.1
  • SFPI1
  • SFPI131 kDa transforming protein
  • SPI-1 proto-oncogene
  • SPI1
  • Spi-1
  • SPI-A
  • spleen focus forming virus (SFFV) proviral integration oncogene spi1,31 kDa-transforming protein
  • transcription factor PU.1

Background

PU.1/Spi1 encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Bioinformatics

Gene Symbol SPI1