PU.1/Spi-1 Antibodies
We offer PU.1/Spi-1 Antibodies for use in common research applications: ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Intracellular Staining by Flow Cytometry, Peptide ELISA, Sandwich ELISA, Western Blot. Each PU.1/Spi-1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PU.1/Spi-1 Antibodies can be used in a variety of model species: Bovine, Canine, Equine, Human, Mouse, Primate. Use the list below to choose the PU.1/Spi-1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PU.1/Spi-1 polyclonal antibodies and browse our PU.1/Spi-1 monoclonal antibody catalog.
Alternate Names for PU.1/Spi-1 Antibodies
anti-PU.1/Spi-1 antibody, anti-SPI1 antibody, anti-hematopoietic transcription factor PU.1 antibody, anti-OF antibody, anti-PU.1 antibody, anti-SFPI131 kDa transforming protein antibody, anti-SPI-1 antibody, anti-SPI-1 proto-oncogene antibody, anti-SPI-A antibody, anti-spleen focus forming virus (SFFV) proviral integration oncogene spi1,31 kDa-transforming protein antibody, anti-transcription factor PU.1 antibody
11 Results for "pu1-spi-1" in Primary Antibodies
![Western Blot: PU.1/Spi-1 Antibody [NBP2-27163] - PU.1/Spi1 Antibody [NBP2-27163] - Analysis of PU.1/Spi1 in U937 cell lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-27163.](http://images.novusbio.com/fullsize/PU.1-Spi-1-Antibody-Western-Blot-NBP2-27163-img0005.jpg "Western Blot: PU.1/Spi-1 Antibody [NBP2-27163] - PU.1/Spi1 Antibody [NBP2-27163] - Analysis of PU.1/Spi1 in U937 cell lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-27163.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: PU.1/Spi-1 Antibody [NBP2-27163] - PU.1/Spi1 Antibody [NBP2-27163] - Intracellular analysis of PU.1 in mouse RAW cells (top) and PMA treated (20 ng/ml, overnight) human ThP1 cells (bottom) using 1.25 ug/10^6 cells. Green peak represents isotype control this antibody ; red represents anti-PU.1 antibody.](http://images.novusbio.com/fullsize/PU.1-Spi-1-Antibody-Flow-Cytometry-NBP2-27163-img0006.jpg)
![Western Blot: PU.1/Spi1 Antibody [NBP2-27163] - analysis of 293T cell lysate (30ug) using anti-PU.1/Spi1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/PU.1-Spi1-Antibody-Western-Blot-NBP2-27163-img0003.jpg)
![Western Blot: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Analysis of THP-1 cell lysate using PU.1 Mouse Monoclonal Antibody (PU1/2146).](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-PU1-2146-Western-Blot-NBP2-75738-img0005.jpg "Western Blot: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Analysis of THP-1 cell lysate using PU.1 Mouse Monoclonal Antibody (PU1/2146).")
![Immunocytochemistry/Immunofluorescence: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Immunofluorescence Analysis of PFA-fixed Ramos cells labeling PU.1 with PU.1/Spi-1 Antibody (PU1/2146) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is labeled with Phalloidin (Red).](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-PU1-2146-Immunocytochemistry-Immunofluorescence-NBP2-75738-img0008.jpg)
![Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Formalin-fixed, paraffin-embedded human Spleen stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-PU1-2146-Immunohistochemistry-Paraffin-NBP2-75738-img0004.jpg)
![Flow Cytometry: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Flow Cytometric Analysis of PFA-fixed Ramos cells. PU.1/Spi-1 Antibody (PU1/2146) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-PU1-2146-Flow-Cytometry-NBP2-75738-img0007.jpg)
![Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-PU1-2146-Immunohistochemistry-Paraffin-NBP2-75738-img0001.jpg)
![Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-PU1-2146-Immunohistochemistry-Paraffin-NBP2-75738-img0002.jpg)
![Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Formalin-fixed, paraffin-embedded human Lymph Node stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-PU1-2146-Immunohistochemistry-Paraffin-NBP2-75738-img0003.jpg)
![Protein Array: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PU.1/Spi-1 Antibody (PU1/2146)Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-PU1-2146-Protein-Array-NBP2-75738-img0009.jpg)
![Western Blot: PU.1/Spi-1 Antibody [NBP1-00135] - (0.03ug/ml) Analysis of DAUDI lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PU.1-Spi-1-Antibody-Western-Blot-NBP1-00135-img0005.jpg "Western Blot: PU.1/Spi-1 Antibody [NBP1-00135] - (0.03ug/ml) Analysis of DAUDI lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody [NBP1-00135] - (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PU.1-Spi-1-Antibody-Immunohistochemistry-Paraffin-NBP1-00135-img0007.jpg)
![Flow Cytometry: PU.1/Spi-1 Antibody [NBP1-00135] - Analysis of PU.1/Spi1 in p3xFLAG-PU.1 transfected 293T cells (1 million cells) using anti-PU.1/Spi1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/PU.1-Spi-1-Antibody-Flow-Cytometry-NBP1-00135-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: PU.1/Spi-1 Antibody [NBP2-33427] - Staining of human cell line REH shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PU.1-Spi-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33427-img0003.jpg "Immunocytochemistry/Immunofluorescence: PU.1/Spi-1 Antibody [NBP2-33427] - Staining of human cell line REH shows localization to nucleoplasm. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody [NBP2-33427] - Staining in human bone marrow and pancreas tissues using anti-SPI1 antibody. Corresponding SPI1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PU.1-Spi-1-Antibody-Immunohistochemistry-Paraffin-NBP2-33427-img0006.jpg)
![Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody [NBP2-33427] - Staining of human bone marrow shows high expression.](http://images.novusbio.com/fullsize/PU.1-Spi-1-Antibody-Immunohistochemistry-Paraffin-NBP2-33427-img0004.jpg)
![Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody [NBP2-33427] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/PU.1-Spi-1-Antibody-Immunohistochemistry-Paraffin-NBP2-33427-img0005.jpg)
![Western Blot: PU.1/Spi-1 Antibody (S02-1D7) [NBP3-19911] - Western blot detection of PU.1/Spi1 in CHO-K1, C6 cell lysates using NBP3-19911 (1:1000 diluted). Predicted band size: 31kDa. Observed band size: 31kDa.](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-S02-1D7-Western-Blot-NBP3-19911-img0002.jpg "Western Blot: PU.1/Spi-1 Antibody (S02-1D7) [NBP3-19911] - Western blot detection of PU.1/Spi1 in CHO-K1, C6 cell lysates using NBP3-19911 (1:1000 diluted). Predicted band size: 31kDa. Observed band size: 31kDa.")
![Immunocytochemistry/Immunofluorescence: PU.1/Spi-1 Antibody (S02-1D7) [NBP3-19911] - Immunocytochemistry of PU.1/Spi1 (green) in Raw264. 7 using NBP3-19911 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-S02-1D7-Immunocytochemistry-Immunofluorescence-NBP3-19911-img0001.jpg)
![Western Blot: PU.1/Spi-1 Antibody (PD00-14) [NBP3-32878] - Western blot analysis of PU.1/Spi-1 on different lysates with Rabbit anti-PU.1/Spi-1 antibody (NBP3-32878) at 1/500 dilution. Lane 1: Daudi cell lysate Lane 2: THP-1 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 31 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32878) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32878_rabbit-pu-1-spi-1-mab-pd00-14-288202412461641.jpg "Western Blot: PU.1/Spi-1 Antibody (PD00-14) [NBP3-32878] - Western blot analysis of PU.1/Spi-1 on different lysates with Rabbit anti-PU.1/Spi-1 antibody (NBP3-32878) at 1/500 dilution. Lane 1: Daudi cell lysate Lane 2: THP-1 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 31 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32878) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PU.1/Spi-1 Antibody (PD00-14) [NBP3-32878] - Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Rabbit anti-PU.1/Spi-1 antibody (NBP3-32878) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32878) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32878_rabbit-pu-1-spi-1-mab-pd00-14-298202414115448.jpg)
![Western Blot: PU.1/Spi-1 Antibody (S07-4D3) [NBP3-14966] - Western blot detection of PU. 1/Spi-1 in Daudi, mouse liver cell lysates using PU. 1/Spi-1 Rabbit mAb (1:1000 diluted). Predicted band size: 31kDa. Observed band size: 42kDa.](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-S07-4D3-Western-Blot-NBP3-14966-img0001.jpg "Western Blot: PU.1/Spi-1 Antibody (S07-4D3) [NBP3-14966] - Western blot detection of PU. 1/Spi-1 in Daudi, mouse liver cell lysates using PU. 1/Spi-1 Rabbit mAb (1:1000 diluted). Predicted band size: 31kDa. Observed band size: 42kDa.")