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Spi-B Antibody

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Spi-B was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse Spi-B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7204) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Spi-B was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse Spi-B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7204) at 15 µg/mL for 3 hours at room temperature. Cells were stained ...read more
Mouse splenocytes were stained with Sheep Anti-Mouse Spi-B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7204) followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127) and ...read more
OPGhigh M cells cluster more in cecal patches than in Peyer’s patches.a Whole-mount immunostaining of the FAE of Peyer’s patches (left) and cecal patches (right) for OPG (green) and Spi-B (red). Nuclei were stained ...read more
RANKL–RANK signaling is stimulated in the gut epithelia of Opg−/− mice.a GP2+ cells (red) are more effectively induced in the cecal epithelium (CE) and ileal villi (VE) of Opg−/− mice by RANKL administration. ...read more
RANKL–RANK signaling is stimulated in the gut epithelia of Opg−/− mice.a GP2+ cells (red) are more effectively induced in the cecal epithelium (CE) and ileal villi (VE) of Opg−/− mice by RANKL administration. ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications CyTOF-ready, Flow, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF7204-SP
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Spi-B Antibody Summary

Immunogen
E. coli-derived recombinant mouse Spi-B
Tyr18-Glu167 (Tyr110Phe)
Accession # O35906
Specificity
Detects mouse Spi-B in direct ELISAs. In direct ELISAs, less than 2% cross-reactivity with recombinant human Spi-B is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
SPIB
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
Publications
Read Publications using
AF7204 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Spi-B Antibody

  • Spi-B transcription factor (Spi-1/PU.1 related)
  • SpiB
  • Spi-B
  • transcription factor Spi-B

Background

Spi-B (Transcription factor Spi-B) is a 33-45 kDa member of the ets family of transcription factors. It is found in hematopoietic cells such as B cells and plasmacytoid dendritic cells (DC). In transitional B cells, Spi-B promotes their differentiation into follicular (naïve) B cells. In hematopoietic stem cells, Spi-B stimulates the generation of IFN-producing plasmacytoid DC at the expense of T, B and NK cell development. Mouse Spi-B is 267 amino acids (aa) in length. It contains a dual transactivation region (aa 1-62), plus a PEST domain (aa 110-170) and an Ets DNA-binding domain (aa 174-257). There are two isoform variants. One shows a nine aa substitution for aa 1-8, while a second possesses an 18 aa insertion after Leu17. Over aa 18-167, mouse Spi-B shares 91% and 74% aa identity with rat and human Spi-B, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Spi-B Antibody (AF7204)(8)

We have publications tested in 2 confirmed species: Mouse, Transgenic Mouse.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-Fr, IHC-P.


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ICC/IF
(1)
IHC
(4)
IHC-Fr
(1)
IHC-P
(1)
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Mouse
(6)
Transgenic Mouse
(1)
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Showing Publications 1 - 8 of 8.
Publications using AF7204 Applications Species
MV Luna Velez, HK Neikes, RR Snabel, Y Quint, C Qian, A Martens, GJC Veenstra, MR Freeman, SJ van Heerin, M Vermeulen ONECUT2 regulates RANKL-dependent enterocyte and microfold cell differentiation in the small intestine; a multi-omics study Nucleic Acids Research, 2023-02-22;0(0):. 2023-02-22 [PMID: 36625255] (IHC, Mouse) IHC Mouse
K Usami, K Niimi, A Matsuo, Y Suyama, Y Sakai, S Sato, K Fujihashi, H Kiyono, S Uchino, M Furukawa, J Islam, K Ito, T Moriya, Y Kusumoto, M Tomura, RC Hovey, J Sugawara, H Yoneyama, H Kitazawa, K Watanabe, H Aso, T Nochi The gut microbiota induces Peyer's-patch-dependent secretion of maternal IgA into milk Cell Reports, 2021-09-07;36(10):109655. 2021-09-07 [PMID: 34496253] (IHC, Transgenic Mouse) IHC Transgenic Mouse
S Kimura, Y Nakamura, N Kobayashi, K Shiroguchi, E Kawakami, M Mutoh, H Takahashi-, T Yamada, M Hisamoto, M Nakamura, N Udagawa, S Sato, T Kaisho, T Iwanaga, K Hase Osteoprotegerin-dependent M cell self-regulation balances gut infection and immunity Nat Commun, 2020-01-13;11(1):234. 2020-01-13 [PMID: 31932605] (ICC/IF, Mouse) ICC/IF Mouse
Nicole Y. Lai, Melissa A. Musser, Felipe A. Pinho-Ribeiro, Pankaj Baral, Amanda Jacobson, Pingchuan Ma et al. Gut-Innervating Nociceptor Neurons Regulate Peyer’s Patch Microfold Cells and SFB Levels to Mediate Salmonella Host Defense Cell 1/1/2020 [PMID: 31813624]
A Sehgal, DS Donaldson, C Pridans, KA Sauter, DA Hume, NA Mabbott The role of CSF1R-dependent macrophages in control of the intestinal stem-cell niche Nat Commun, 2018-03-28;9(1):1272. 2018-03-28 [PMID: 29593242] (IHC, Mouse) IHC Mouse
T Kanaya, S Sakakibara, T Jinnohara, M Hachisuka, N Tachibana, S Hidano, T Kobayashi, S Kimura, T Iwanaga, T Nakagawa, T Katsuno, N Kato, T Akiyama, T Sato, IR Williams, H Ohno Development of intestinal M cells and follicle-associated epithelium is regulated by TRAF6-mediated NF-?B signaling J. Exp. Med., 2018-01-16;0(0):. 2018-01-16 [PMID: 29339448] (IHC-P, Mouse) IHC-P Mouse
Increased Abundance of M Cells in the Gut Epithelium Dramatically Enhances Oral Prion Disease Susceptibility PLoS Pathog, 2016-12-14;12(12):e1006075. 2016-12-14 [PMID: 27973593] (IHC-Fr, Mouse) IHC-Fr Mouse
c-Rel is dispensable for the differentiation and functional maturation of M cells in the follicle-associated epithelium Immunobiology, 2016-09-18;0(0):. 2016-09-18 [PMID: 27663963] (IHC, Mouse) IHC Mouse

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Bioinformatics

Gene Symbol SPIB
Uniprot