![Western Blot: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Analysis of THP-1 cell lysate using PU.1 Mouse Monoclonal Antibody (PU1/2146).](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-PU1-2146-Western-Blot-NBP2-75738-img0005.jpg "Western Blot: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Analysis of THP-1 cell lysate using PU.1 Mouse Monoclonal Antibody (PU1/2146).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, MA |
| Clone | PU1/2146 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-75766) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. |
| Immunogen | Recombinant fragment (around aa 16-170) of human PU.1/Spi-1 protein (Exact sequence is proprietary) (Uniprot: P17947 ) |
| Localization | Nuclear |
| Marker | B-Cell Marker |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SPI1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 40 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
![Western Blot Ets-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Ets-1_AF7284_Western_Blot_11854.jpg)
![Immunohistochemistry Ets-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Ets1_AF7284_Immunohistochemistry_22698.jpg)
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Immunofluorescence Analysis of PFA-fixed Ramos cells labeling PU.1 with PU.1/Spi-1 Antibody (PU1/2146) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is labeled with Phalloidin (Red).](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-PU1-2146-Immunocytochemistry-Immunofluorescence-NBP2-75738-img0008.jpg "Immunocytochemistry/Immunofluorescence: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Immunofluorescence Analysis of PFA-fixed Ramos cells labeling PU.1 with PU.1/Spi-1 Antibody (PU1/2146) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is labeled with Phalloidin (Red).")
![Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Formalin-fixed, paraffin-embedded human Spleen stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-PU1-2146-Immunohistochemistry-Paraffin-NBP2-75738-img0004.jpg "Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Formalin-fixed, paraffin-embedded human Spleen stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).")
![Flow Cytometry: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Flow Cytometric Analysis of PFA-fixed Ramos cells. PU.1/Spi-1 Antibody (PU1/2146) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-PU1-2146-Flow-Cytometry-NBP2-75738-img0007.jpg "Flow Cytometry: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Flow Cytometric Analysis of PFA-fixed Ramos cells. PU.1/Spi-1 Antibody (PU1/2146) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).")
![Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-PU1-2146-Immunohistochemistry-Paraffin-NBP2-75738-img0001.jpg "Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).")
![Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-PU1-2146-Immunohistochemistry-Paraffin-NBP2-75738-img0002.jpg "Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).")
![Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Formalin-fixed, paraffin-embedded human Lymph Node stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-PU1-2146-Immunohistochemistry-Paraffin-NBP2-75738-img0003.jpg "Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Formalin-fixed, paraffin-embedded human Lymph Node stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).")
![Protein Array: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PU.1/Spi-1 Antibody (PU1/2146)Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-PU1-2146-Protein-Array-NBP2-75738-img0009.jpg "Protein Array: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PU.1/Spi-1 Antibody (PU1/2146)Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.")
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