Home » PU.1/Spi-1 » PU.1/Spi-1 Antibodies » PU.1/Spi-1 Antibody (PU1/2146) [mFluor Violet 450 SE]
Novus Biologicals products are now on bio-techne.com

PU.1/Spi-1 Antibody (PU1/2146) [mFluor Violet 450 SE]

 Submit a Review

Images

 
(
Enlarge)
PU.1/Spi-1 Antibody (PU1/2146) [mFluor Violet 450 SE] [NBP2-75766MFV450] - Vial of mFluor Violet 450 conjugated antibody. mFluor Violet 450 is optimally excited at 406 nm by the Violet laser (405 nm) and has an emission maximum of 445 nm.
PU.1/Spi-1 Antibody (PU1/2146) [mFluor Violet 450 SE] [NBP2-75766MFV450] - Vial of mFluor Violet 450 conjugated antibody. mFluor Violet 450 is optimally excited at 406 nm by the Violet laser (405 nm) and has an emission ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, MA
Clone
PU1/2146
Clonality
Monoclonal
Host
Mouse
Conjugate
mFluor Violet 450 SE

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-75766AF350
Alexa Fluor 405
NBP2-75766AF405
Alexa Fluor 488
NBP2-75766AF488
Alexa Fluor 532
NBP2-75766AF532
Alexa Fluor 594
NBP2-75766AF594
Alexa Fluor 647
NBP2-75766AF647
Alexa Fluor 700
NBP2-75766AF700
Alexa Fluor 750
NBP2-75766AF750
Allophycocyanin
NBP2-75766APC
Biotin
NBP2-75766B
DyLight 350
NBP2-75766UV
DyLight 405
NBP2-75766V
DyLight 488
NBP2-75766G
DyLight 550
NBP2-75766R
DyLight 594
NBP2-75766DL594
DyLight 650
NBP2-75766C
DyLight 680
NBP2-75766FR
DyLight 755
NBP2-75766IR
FITC
NBP2-75766F
HRP
NBP2-75766H
Janelia Fluor 549
NBP2-75766JF549
Janelia Fluor 646
NBP2-75766JF646
PE
NBP2-75766PE
PerCP
NBP2-75766PCP
Unconjugated
NBP2-75738-100ug
mFluor Violet 500 SE
NBP2-75766MFV500
mFluor Violet 610 SE
NBP2-75766MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-75738-100ug
Azide and BSA Free
NBP2-75766
100% Guarantee on every product

PU.1/Spi-1 Antibody (PU1/2146) [mFluor Violet 450 SE] Summary

Immunogen
Recombinant fragment (around aa 16-170) of human PU.1/Spi-1 protein (Exact sequence is proprietary) (Uniprot: P17947 )
Localization
Nuclear
Marker
B-Cell Marker
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SPI1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Protein Array
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for PU.1/Spi-1 Antibody (PU1/2146) [mFluor Violet 450 SE]

  • hematopoietic transcription factor PU.1
  • OF
  • PU.1
  • SFPI1
  • SFPI131 kDa transforming protein
  • SPI-1 proto-oncogene
  • SPI1
  • Spi-1
  • SPI-A
  • spleen focus forming virus (SFFV) proviral integration oncogene spi1,31 kDa-transforming protein
  • transcription factor PU.1

Background

PU.1/Spi1 encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

GATA-1 Antibody
(4F5) - BSA Free
NBP1-47492
Immunohistochemistry GATA-1 Antibody (4F5) - BSA Free
Immunocytochemistry/ Immunofluorescence GATA-1 Antibody (4F5) - BSA Free
Immunohistochemistry-Paraffin GATA-1 Antibody (4F5) - BSA Free
(1 Review)
(2 Publications)
Species: Pm, Hu
Applications: ChIP, ELISA, ICC/IF, IHC, IHC-P, WB
c-jun [p Ser63]
Antibody (1018Y) [Unc ...
MAB8930
Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
(1 Review)
(3 Publications)
Species: Hu
Applications: ICC, Simple Western, WB
Spi-B Antibody
AF7204
Immunocytochemistry Spi-B Antibody
Intracellular Staining by Flow Cytometry Spi-B Antibody
Immunocytochemistry/ Immunofluorescence Spi-B Antibody
(8 Publications)
Species: Mu
Applications: CyTOF-ready, ICC, ICFlow
IRF8 Antibody
AF5117
Western Blot IRF8 Antibody
Immunohistochemistry IRF8 Antibody
(1 Publication)
Species: Hu
Applications: IHC, WB
RUNX1/CBFA2
Antibody
NBP1-89105
Immunocytochemistry/ Immunofluorescence RUNX1/CBFA2 Antibody
Immunohistochemistry-Paraffin RUNX1/CBFA2 Antibody
Immunohistochemistry-Paraffin RUNX1/CBFA2 Antibody
(1 Review)
(2 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Ets-1 Antibody
[Unconjugated]
AF7284
Western Blot Ets-1 Antibody [Unconjugated]
Immunohistochemistry Ets-1 Antibody [Unconjugated]
Species: Hu, Mu, Rt
Applications: IHC, WB
SP1 Antibody
NB600-232
Western Blot SP1 Antibody
Immunohistochemistry-Paraffin SP1 Antibody
Western Blot SP1 Antibody
(1 Review)
(7 Publications)
Species: Hu, Mu
Applications: ChIP, IHC, IHC-P, IP, WB
GM-CSF
[Unconjugated]
7954-GM/CF
Bioactivity GM-CSF [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
c-Fos Antibody
(2H2)
NBP2-50037
Knockout Validated c-Fos Antibody (2H2)
Immunocytochemistry/ Immunofluorescence c-Fos Antibody (2H2)
Immunocytochemistry/ Immunofluorescence c-Fos Antibody (2H2)
(1 Review)
(18 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KO, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(803 Publications)
Species: Hu
Applications: BA
Erythropoietin/EPO
[HRP]
MEP00B
N/A Erythropoietin/EPO [HRP]
N/A Erythropoietin/EPO [HRP]
(104 Publications)
Species: Mu
Applications: ELISA
Pax5/BSAP Antibody
(1207C) [Unconjugated ...
MAB3487
Flow Cytometry Pax5/BSAP Antibody (1207C) [Unconjugated]
Western Blot Pax5/BSAP Antibody (1207C) [Unconjugated]
Immunohistochemistry Pax5/BSAP Antibody (1207C) [Unconjugated]
(1 Publication)
Species: Hu, Mu
Applications: Flow, ICC, IHC, Simple Western, WB
PSG1 [HRP]
DPSG10
N/A PSG1 [HRP]
N/A PSG1 [HRP]
(2 Publications)
Species: Hu
Applications: ELISA
COCO/DAND5
[Unconjugated]
3047-CC
COCO/DAND5 [Unconjugated]
(4 Publications)
Species: Hu
Applications: BA
SCL/Tal1 Antibody
(OTI5H1)
NBP2-01488
Western Blot SCL/Tal1 Antibody (OTI5H1)
Flow Cytometry SCL/Tal1 Antibody (OTI5H1)
Immunocytochemistry/ Immunofluorescence SCL/Tal1 Antibody (OTI5H1)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
IL-3 [Unconjugated]
203-IL
SDS-PAGE IL-3 [Unconjugated]
Bioactivity IL-3 [Unconjugated]
SEC-MALS IL-3 [Unconjugated]
(203 Publications)
Species: Hu
Applications: BA
GM-CSF
[Unconjugated]
7954-GM/CF
Bioactivity GM-CSF [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
CD11b Antibody -
BSA Free
NB110-89474
Immunocytochemistry/ Immunofluorescence CD11b Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence CD11b Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence CD11b Antibody - BSA Free
(12 Reviews)
(104 Publications)
Species: Bv, Ce, ChHa, Hu, Pm, Mu, Rt, RM
Applications: Dual ISH-IHC, Flow-CS, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, ISH, Simple Western, Single-Cell Western, WB

Publications for PU.1/Spi-1 Antibody (NBP2-75766MFV450) (0)

There are no publications for PU.1/Spi-1 Antibody (NBP2-75766MFV450).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PU.1/Spi-1 Antibody (NBP2-75766MFV450) (0)

There are no reviews for PU.1/Spi-1 Antibody (NBP2-75766MFV450). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PU.1/Spi-1 Antibody (NBP2-75766MFV450) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional PU.1/Spi-1 Products

Research Areas for PU.1/Spi-1 Antibody (NBP2-75766MFV450)

Find related products by research area.

Blogs on PU.1/Spi-1

There are no specific blogs for PU.1/Spi-1, but you can read our latest blog posts.
Earn Double Rewards for Data!

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PU.1/Spi-1 Antibody (PU1/2146) [mFluor Violet 450 SE] and receive a gift card or discount.


Bioinformatics

Gene Symbol SPI1