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PTPN14/PTPD2 Antibody (448701)

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Western blot shows lysates of DU145 human prostate carcinoma cell line, HT-29 human colon adenocarcinoma cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human PTPN14 Monoclonal Antibody (Catalog # MAB4458) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTPN14 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of DU145 human prostate carcinoma cell line, HT-29 human colon adenocarcinoma cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human PTPN14 ...read more
PTPN14 was detected in immersion fixed paraffin-embedded sections of human placenta using Human PTPN14 Monoclonal Antibody (Catalog # MAB4458) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
448701
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB4458-SP
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PTPN14/PTPD2 Antibody (448701) Summary

Immunogen
E. coli-derived recombinant human PTPN14
Leu329-Leu900
Accession # Q15678.2
Specificity
Detects endogenous human PTPN14 in Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) PTPN13 or rhPTPN22.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PTPN14
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB4458 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PTPN14/PTPD2 Antibody (448701)

  • EC 3.1.3
  • EC 3.1.3.48
  • MGC126803
  • PEZ
  • PEZcytoskeletal-associated protein tyrosine phosphatase
  • protein tyrosine phosphatase, non-receptor type 14
  • Protein-tyrosine phosphatase pez
  • PTP36
  • PTPN14
  • tyrosine-protein phosphatase non-receptor type 14

Background

Protein Tyrosine Phosphatase, Non-receptor type 14 (PTPN14), also called Phosphatase with Ezrin Domain (PEZ) and PTP36, is a 135 kDa cytosolic protein that dephosphorylates tyrosine residues in proteins. PTPN14’s Ezrin domain is homologous to erythrocyte Band 4.1 protein, targeting the phosphatase to cytoskeletal elements such as actin. PTPN14 associates with beta-catenin in adhesion zones in cells that are touching, but is nuclear in isolated, proliferating cells. Loss-of-function mutations in PTPN14 are found in 26% of colorectal cancers, suggesting it is a tumor suppressor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PTPN14/PTPD2 Antibody (MAB4458)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: IHC, IHC-P, Western Blot.


Filter By Application
IHC
(1)
IHC-P
(1)
Western Blot
(6)
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 9 of 9.
Publications using MAB4458 Applications Species
LT Wang, MÈ Proulx, AD Kim, V Lelarge, L McCaffrey A proximity proteomics screen in three-dimensional spheroid cultures identifies novel regulators of lumen formation Scientific Reports, 2021-11-23;11(1):22807. 2021-11-23 [PMID: 34815476] (IHC, Western Blot, Human) IHC, Western Blot Human
NI Díaz-Valdi, J Díaz, P Contreras, A Campos, V Rojas-Celi, RA Burgos-Rav, L Lobos-Gonz, VA Torres, VI Perez, B Frei, L Leyton, AFG Quest The non-receptor tyrosine phosphatase type 14 blocks caveolin-1-enhanced cancer cell metastasis Oncogene, 2020-03-09;0(0):. 2020-03-09 [PMID: 32152405] (Western Blot, Human) Western Blot Human
J Szelachows, P Donizy, K Ratajczak-, A Halon, D Zielecka-D, K Lichon, A Maciejczyk, E Lata-Wozni, A Piotrowska, R Matkowski The effect of YAP expression in tumor cells and tumor stroma on the prognosis of patients with squamous cell carcinoma of the oral cavity floor and oral surface of the tongue Oncol Lett, 2019-07-31;18(4):3561-3570. 2019-07-31 [PMID: 31579068] (IHC-P, Human) IHC-P Human
Gareth W Fearnley, Katherine A Young, James R Edgar, Robin Antrobus, Iain M Hay, Wei-Ching Liang et al. The homophilic receptor PTPRK selectively dephosphorylates multiple junctional regulators to promote cell–cell adhesion eLife 3/29/2019 [PMID: 30924770]
Fan G, Aleem S, Yang M, Miller W, Tonks N Protein-tyrosine Phosphatase and Kinase Specificity in Regulation of SRC and Breast Tumor Kinase. J Biol Chem, 2015-04-20;290(26):15934-47. 2015-04-20 [PMID: 25897081] (Western Blot, Human) Western Blot Human
Ramesh M, Krishnan N, Muthuswamy S, Tonks N A novel phosphatidic acid-protein-tyrosine phosphatase D2 axis is essential for ERBB2 signaling in mammary epithelial cells. J Biol Chem, 2015-02-13;290(15):9646-59. 2015-02-13 [PMID: 25681440] (Western Blot, Human) Western Blot Human
Fan G, Lin G, Lucito R, Tonks N Protein-tyrosine phosphatase 1B antagonized signaling by insulin-like growth factor-1 receptor and kinase BRK/PTK6 in ovarian cancer cells. J Biol Chem, 2013-06-28;288(34):24923-34. 2013-06-28 [PMID: 23814047] (Western Blot, Human) Western Blot Human
Chrysiis Michaloglou, Waltraut Lehmann, Typhaine Martin, Clara Delaunay, Andreas Hueber, Louise Barys et al. The Tyrosine Phosphatase PTPN14 Is a Negative Regulator of YAP Activity PLoS ONE 4/16/2013 [PMID: 23613971]
Huang JM, Nagatomo I YAP modifies cancer cell sensitivity to EGFR and survivin inhibitors and is negatively regulated by the non-receptor type protein tyrosine phosphatase 14. 2012-06-11;0(0):. 2012-06-11 [PMID: 22689061] (Western Blot, Human) Western Blot Human

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Bioinformatics

Gene Symbol PTPN14
Uniprot