![Western Blot: Ezrin Antibody [NBP2-16396] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [N2C2], Internal diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody](http://images.novusbio.com/fullsize/Ezrin-Antibody-Western-Blot-NBP2-16396-img0010.jpg "Western Blot: Ezrin Antibody [NBP2-16396] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [N2C2], Internal diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody")
| Reactivity | Hu, Mu, Rt, Ze, Bv, Ca, Ch, Pm, RbSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Ezrin. The exact sequence is proprietary. |
| Localization | Apical cell membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection; microvillus membrane; ruffle membrane; Cytoplasm; cell cortex |
| Predicted Species | Bovine (96%), Rabbit (94%), Chimpanzee (99%), Chicken (92%), Canine (98%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | EZR |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 69 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
WB | Human | 10/16/2015 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Ezrin Antibody (NBP2-16396)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Ezrin Antibody [NBP2-16396] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ezrin stained by Ezrin antibody [N2C2], Internal diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Ezrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16396-img0014.jpg "Immunocytochemistry/Immunofluorescence: Ezrin Antibody [NBP2-16396] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ezrin stained by Ezrin antibody [N2C2], Internal diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-16396] - Rat duodenum. Ezrin antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Ezrin-Antibody-Immunohistochemistry-Paraffin-NBP2-16396-img0008.jpg "Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-16396] - Rat duodenum. Ezrin antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunocytochemistry/Immunofluorescence: Ezrin Antibody [NBP2-16396] - HCT116 cells.](http://images.novusbio.com/fullsize/Ezrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16396-img0005.jpg "Immunocytochemistry/Immunofluorescence: Ezrin Antibody [NBP2-16396] - HCT116 cells.")
![Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-16396] - C2C12 xenograft, using Ezrin antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Ezrin-Antibody-Immunohistochemistry-Paraffin-NBP2-16396-img0002.jpg "Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-16396] - C2C12 xenograft, using Ezrin antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-16396] - Human colon carcinoma, using Ezrin antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Ezrin-Antibody-Immunohistochemistry-Paraffin-NBP2-16396-img0003.jpg "Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-16396] - Human colon carcinoma, using Ezrin antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![IHC-Whole-Mount- Ezrin Antibody [NBP2-16396]--Fixed zebrafish embryos. Ezrin antibody [N2C2], Internal dilution: 1:200.](http://images.novusbio.com/fullsize/antibody/nbp2-16396_rabbit-polyclonal-ezrin-antibody-immunohistochemistry-whole-mount-17720238317..jpg "IHC-Whole-Mount- Ezrin Antibody [NBP2-16396]--Fixed zebrafish embryos. Ezrin antibody [N2C2], Internal dilution: 1:200.")
![IHC-Whole-Mount- Ezrin Antibody [NBP2-16396] - Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo. Green: Ezrin stained by Ezrin antibody [N2C2], Internal diluted at 1:100. Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70C](http://images.novusbio.com/fullsize/antibody/nbp2-16396_rabbit-polyclonal-ezrin-antibody-immunohistochemistry-whole-mount-177202383248.jpg "IHC-Whole-Mount- Ezrin Antibody [NBP2-16396] - Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo. Green: Ezrin stained by Ezrin antibody [N2C2], Internal diluted at 1:100. Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70C")
![Western Blot: Ezrin Antibody [NBP2-16396] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301)) was used to detect the primary antibody](http://images.novusbio.com/fullsize/antibody/nbp2-16396_rabbit-polyclonal-ezrin-antibody-western-blot-177202383425..jpg "Western Blot: Ezrin Antibody [NBP2-16396] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301)) was used to detect the primary antibody")
![Western Blot: Ezrin Antibody [NBP2-16396] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-16396_rabbit-polyclonal-ezrin-antibody-western-blot-177202383550..jpg "Western Blot: Ezrin Antibody [NBP2-16396] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
