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Moesin Antibody (MSN/491)

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Western Blot: Moesin Antibody (MSN/491) [NBP2-32875] - Western Blot Analysis of human PC3 cell lysate using Moesin Antibody (MSN/491).
Immunocytochemistry/ Immunofluorescence: Moesin Antibody (MSN/491) [NBP2-32875] - Immunofluorescence Analysis of PFA-fixed HeLa cells labeling Moesin with Moesin Antibody (MSN/491) followed by Goat anti-Mouse IgG-CF488 ...read more
Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) [NBP2-32875] - Fomalin-paraffin Rat colon stained with Moesin MAb (MSN/491)
Flow Cytometry: Moesin Antibody (MSN/491) [NBP2-32875] - Flow Cytometric Analysis of K562 cells using Moesin Antibody (MSN/491) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Western Blot: Moesin Antibody (MSN/491) [NBP2-32875] - Analysis using the Azide and BSA Free version of NBP2-32875. Detection of Moesin in human HT29 Cells.
Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) [NBP2-32875] - Formalin-paraffin human placenta stained with Moesin MAb (MSN/491)
Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) [NBP2-32875] - Formalin-paraffin human Melanoma stained with Moesin Mab (MSN/491)
Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) [NBP2-32875] - Formalin-paraffin Rat Lung stained with Moesin MAb (MSN/491)
Simple Western: Moesin Antibody (MSN/491) [NBP2-32875] - Simple Western lane view shows a specific band for Moesin in 0.1 mg/ml of HUVEC lysate. This experiment was performed under reducing conditions using the 12-230 ...read more
Simple Western: Moesin Antibody (MSN/491) [NBP2-32875] - Electropherogram image of the corresponding Simple western lane view. Moesin antibody was used at 1 ug/ml dilution on HUVEC lysate(s) respectively.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC
Clone
MSN/491
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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Moesin Antibody (MSN/491) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34685)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant full-length human Moesin protein (Uniprot: P26038)
Localization
Cytoplasmic & Cell Surface
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MSN
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 2-4 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Simple Western 1.0 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-32875.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Moesin Antibody (MSN/491)

  • Membrane-organizing extension spike protein
  • moesin

Background

Moesin (membrane-organizing extension spike protein) has previously been characterized as a possible receptor protein for heparan sulfate and also as a cytoskeletal linker protein that stabilizes cell surface microvilli, filopodia and lamellipodia. Data indicate that moesin is identical to the 77-kDa band that copurifies with ezrin in its isolation from human placenta (1). Members of the ezrin-radixin-moesin (ERM) family of membrane-cytoskeletal linking proteins have NH2- and COOH-terminal domains that associate with the plasma membrane and the actin cytoskeleton, respectively (2). It has been demonstrated that ezrin-radixin-moesin proteins are rapidly inactivated after antigen recognition through a Vav1-Rac1 pathway. The resulting disanchoring of the cortical actin cytoskeleton from the plasma membrane decreased cellular rigidity, leading to more efficient T cell-antigen-presenting cell conjugate formation (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Moesin Antibody (NBP2-32875)(1)

We have publications tested in 1 application: Western Blot.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Moesin Antibody (NBP2-32875). (Showing 1 - 2 of 2 FAQ).

  1. I am looking to use shRNA to inhibit Moesin expression. I have had people advise me that my initial MOI should be low as 'less is more' and 'a little goes a long way' in terms of siRNA. I was wondering if you could elaborate on this for me and explain why my initial MOI should be low.
    • The reason for a low MOI is most likely because RNAi is a very strong and efficient technique. Wikipedia does a good job of explaining <a href="http://en.wikipedia.org/wiki/RNA_interference" target="_blank">RNA interference</a>. However, I would imagine that in a cell, there will be at most 1-2 copies of the gene mRNA present at any given time, unless you're dealing with a highly expressed protein such as Actin, where I would imagine silencing Actin would be lethal to the cell. I can imagine a few reasons to not use too much siRNA. First, it is expensive, so you don't want to waste it. Second, using too much would cause there to be a lot of non-translatable RNA present in the cell, which could trigger an immune response, as the presence of uncapped RNAs can indicate presence of a virus and one of the TLRs may respond to this.
  2. What is the optimal dilution of this item (NBP2-32875-0.1ml) for use in IHC-P?
    • NBP2-32875-0.1ml was supplied at 200ug/ml concentration. The suggested concentration range for IHC-P is 0.5-1.0ug/ml, which will be a dilution range of 1:100-1:200

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MSN
Uniprot