![Western Blot: PPAR gamma/NR1C3 Antibody [NB120-19481] - Dnmt3a1 decreases lipid droplet accumulation in the early stage of adipocyte differentiation. Relative mRNA level of some general genes involved in adipogenesis or energy homeostasis after transfection with si-Dnmt3a1 and si-NC into cells for 48 h. Values represent mean +- SEM from four separate experiments. *P less than 0.05, **P less than 0.01, and ***P less than 0.001. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fphys.2018.01270/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PPAR-gamma-NR1C3-Antibody-Western-Blot-NB120-19481-img0001.jpg "Western Blot: PPAR gamma/NR1C3 Antibody [NB120-19481] - Dnmt3a1 decreases lipid droplet accumulation in the early stage of adipocyte differentiation. Relative mRNA level of some general genes involved in adipogenesis or energy homeostasis after transfection with si-Dnmt3a1 and si-NC into cells for 48 h. Values represent mean +- SEM from four separate experiments. *P less than 0.05, **P less than 0.01, and ***P less than 0.001. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fphys.2018.01270/full) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, Rt, Po, Bv, Ca, Ch, HaSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | Synthetic peptide corresponding to the C terminus of human PPAR gamma. |
| Localization | Nuclear |
| Specificity | Will recognize both PPARgamma1 and PPARgamma2 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PPARG |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | IHC-P reactivity reported in ( PMID: 25187315). |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS with 0.2% gelatin |
| Preservative | 0.1% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
WB | Mouse | 12/20/2011 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for PPAR gamma/NR1C3 Antibody (NB120-19481)Find related products by research area.
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PPAR gamma - An important target in human metabolism Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells by interacting with members of the nuclear hormone receptor superfamily known as peroxisome proliferator activated receptors (PPARs). There are f... Read full blog post. |
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![Western Blot: PPAR gamma/NR1C3 Antibody [NB120-19481] - Dnmt3a1 decreases lipid droplet accumulation in the early stage of adipocyte differentiation. (A) Representative images of oil red O staining (red) & DAPI staining (blue) after transfection with pSDS-Dnmt3a1 & pSDS-NC for 48 h are showed; scale bar: 25 μm. (B) Lipid droplet content by oil red O staining & extraction method of cells transfected with pSDS-Dnmt3a1 & pSDS-NC for 48 h. Values represent mean ± SEM from three independent experiments. ∗∗P < 0.01. (C) Representative images of oil red O staining (red) & DAPI staining (blue) after transfection with si-Dnmt3a1 & si-NC for 48 h are showed; scale bar: 25 μm. (D) Lipid droplet content by oil red O staining & the extraction method of cells transfected with si-Dnmt3a1 & si-NC for 48 h. Values represent mean ± SEM from three independent experiments. ∗P < 0.05. (E) Relative mRNA level of some general genes implicated in adipogenesis or energy homeostasis induced by pSDS-Dnmt3a1 & pSDS-NC into cells. Values represent mean ± SEM from four separate experiments. ∗P < 0.05, ∗∗P < 0.01, & ∗∗∗P < 0.001. (F) Protein expression of some general adipose genes induced by pSDS-Dnmt3a1 & pSDS-NC into cells. (G) Relative mRNA level of some general genes involved in adipogenesis or energy homeostasis after transfection with si-Dnmt3a1 & si-NC into cells for 48 h. Values represent mean ± SEM from four separate experiments. ∗P < 0.05, ∗∗P < 0.01, & ∗∗∗P < 0.001. (H) Protein expression of some general genes involved in adipogenesis at 48 h after transfection by si-Dnmt3a1 & si-NC into cells. The band intensity of Western blotting was obtained by averaging the data from three independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30333755), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-19481_rabbit-polyclonal-ppar-gamma-nr1c3-antibody-31020241620517.jpg "Western Blot: PPAR gamma/NR1C3 Antibody [NB120-19481] - Dnmt3a1 decreases lipid droplet accumulation in the early stage of adipocyte differentiation. (A) Representative images of oil red O staining (red) & DAPI staining (blue) after transfection with pSDS-Dnmt3a1 & pSDS-NC for 48 h are showed; scale bar: 25 μm. (B) Lipid droplet content by oil red O staining & extraction method of cells transfected with pSDS-Dnmt3a1 & pSDS-NC for 48 h. Values represent mean ± SEM from three independent experiments. ∗∗P < 0.01. (C) Representative images of oil red O staining (red) & DAPI staining (blue) after transfection with si-Dnmt3a1 & si-NC for 48 h are showed; scale bar: 25 μm. (D) Lipid droplet content by oil red O staining & the extraction method of cells transfected with si-Dnmt3a1 & si-NC for 48 h. Values represent mean ± SEM from three independent experiments. ∗P < 0.05. (E) Relative mRNA level of some general genes implicated in adipogenesis or energy homeostasis induced by pSDS-Dnmt3a1 & pSDS-NC into cells. Values represent mean ± SEM from four separate experiments. ∗P < 0.05, ∗∗P < 0.01, & ∗∗∗P < 0.001. (F) Protein expression of some general adipose genes induced by pSDS-Dnmt3a1 & pSDS-NC into cells. (G) Relative mRNA level of some general genes involved in adipogenesis or energy homeostasis after transfection with si-Dnmt3a1 & si-NC into cells for 48 h. Values represent mean ± SEM from four separate experiments. ∗P < 0.05, ∗∗P < 0.01, & ∗∗∗P < 0.001. (H) Protein expression of some general genes involved in adipogenesis at 48 h after transfection by si-Dnmt3a1 & si-NC into cells. The band intensity of Western blotting was obtained by averaging the data from three independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30333755), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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