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POU2F2 Antibody (OCT2/7073R) - Azide and BSA Free

Images

 
Formalin-fixed, paraffin-embedded human tonsil stained with OCT2 antibody (OCT2/7073R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human tonsil stained with OCT2 antibody (OCT2/7073R). Inset: PBS instead of primary antibody; secondary only negative control.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
OCT2/7073R
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

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POU2F2 Antibody (OCT2/7073R) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13743).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant fragment of human POU2F2 protein (around aa 112-297) (exact sequence is proprietary) (Uniprot: P09086)
Localization
Nucleus
Marker
B-Cell Marker
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Gene
POU2F2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for POU2F2 Antibody (OCT2/7073R) - Azide and BSA Free

  • homeobox protein
  • Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2
  • OCT2
  • Octamer-binding protein 2
  • Octamer-binding transcription factor 2
  • OTF2
  • OTF-2
  • POU class 2 homeobox 2
  • POU domain, class 2, transcription factor 2

Background

Octamer transcription factor-2 (Oct-2) is a transcription factor that specifically binds to the octamer motif (5'- ATTTCAT-3'). Mostly expressed in B cells, Oct-2 posses a "leucine zipper" domain and belongs to the POU family of transcription factors (1-3). Additionally, Oct-2 regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol POU2F2