We currently have the SLC22A1 antibody (NBP1-51684), and are in the process of developing a quantitation assay with it. However, we are in need of some kind of standard for the OCT1 protein to generate a standard curve for a colorimetric ELISA. We noticed on the Novus website that the product sheet of another SLC22A1 antibody (NBP1-59464) shows the sequence of the peptide-epitope used to generate that antibody, however we cannot find any sequence data for the immunogen used to generate the antibody that we currently have. The only information we have about this immunogen is that it is a purified recombinant fragment of hSLC22A1 expressed in E. coli. So I guess our question is, do you have any information regarding this immunogen (sequence, size, etc) that you could share with us, or can we order some of the immunogen so that we may use this as a standard for quantitating expressed OCT1? Any information would be greatly appreciated!

Name: SLC22A1 Antibody (2C5) - BSA Free
Brand: Novus Biologicals
SKU: NBP1-51684
Price: 449 USD

Question

Accepted Answer

The immunogen used to create this antibody lies between amino acids 284-347. Unfortunately this immunogen is not available for purchase, however.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, MuSpecies Glossary
Applications	WB, ELISA, Flow, ICC/IF, IHC, MS
Clone	2C5
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Format	BSA Free

Catalog# & Formulation	Size	Price
BSA Free NBP1-51684-0.025ml

Immunogen	Purified recombinant fragment of human SLC22A1 expressed in E. Coli.
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	SLC22A1
Purity	Unpurified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	Ascites
Preservative	0.03% Sodium Azide
Purity	Unpurified

SLC22A1 Antibody (2C5) - BSA Free

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Publications for SLC22A1 Antibody (NBP1-51684)(11)

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Bioinformatics

Publications using NBP1-51684	Applications	Species
Wittern CI, Schr�der S, Jensen O et al. Comprehensive characterization of the OCT1 phenylalanine-244-alanine substitution reveals highly substrate-dependent effects on transporter function. The Journal of biological chemistry 2024-09-27 [PMID: 39342994]
Haas M, Ackermann G, Küpper JH et al. OCT1-dependent uptake of structurally diverse pyrrolizidine alkaloids in human liver cells is crucial for their genotoxic and cytotoxic effects Archives of toxicology 2023-09-07 [PMID: 37676300] (ICC/IF)	ICC/IF
Redeker KM, Jensen O, Gebauer L et al. Atypical Substrates of the Organic Cation Transporter 1 Biomolecules 2022-11-09 [PMID: 36359014] (ICC/IF, Human)	ICC/IF	Human
Meyer MJ, Schreier PCF, Basaran M et al. Amino acids in transmembrane helix 1 confer major functional differences between human and mouse orthologs of the polyspecific membrane transporter OCT1 The Journal of biological chemistry 2022-04-21 [PMID: 35469921] (ICC/IF, Human)	ICC/IF	Human
Kim HI, Raffler J, Lu W et al. Fine Mapping and Functional Analysis Reveal a Role of SLC22A1 in Acylcarnitine Transport Am. J. Hum. Genet. 2017-09-14 [PMID: 28942964] (IF/IHC, Mouse)	IF/IHC	Mouse
Zhang Y, Boxberger KH, Hagenbuch B. Organic anion transporting polypeptide 1B3 can form homo- and hetero-oligomers PLoS ONE 2017-06-23 [PMID: 28644885] (Human)		Human
Sekhar GN, Georgian AR, Sanderson L et al. Organic cation transporter 1 (OCT1) is involved in pentamidine transport at the human and mouse blood-brain barrier (BBB). PLoS ONE. 2017-03-31 [PMID: 28362799] (EM, Mouse)	EM	Mouse
Seitz T, Stalmann R, Dalila N et al. Global genetic analyses reveal strong inter-ethnic variability in the loss of activity of the organic cation transporter OCT1. Genome Med 2015-07-09 [PMID: 26157489] (WB, ICC/IF, Mouse)	WB, ICC/IF	Mouse
Boxberger KH, Hagenbuch B, Lampe JN. Common Drugs Inhibit Human Organic Cation Transporter 1 (OCT1)-Mediated Neurotransmitter Uptake. Drug Metab. Dispos. 2014-01-01 [PMID: 24688079]
Dos Santos Pereira JN et al. The role of membrane transporters in the pharmacokinetics of psychotropic drugs: in vitro studies with special focus on organic cation transporters Thesis. 2014-01-01 (IHC-P, Human)	IHC-P	Human
ROmer S, Meyer MJ, Klein K et al. Effects of a Common Eight Base Pairs Duplication at the Exon 7-Intron 7 Junction on Splicing, Expression, and Function of OCT1 Frontiers in pharmacology 2021-05-07 [PMID: 34025422] (ICC/IF, Human)	ICC/IF	Human
Show All 11 Publications. Collapse Publications.

Gene Symbol	SLC22A1
Entrez	6580(Human)
Uniprot	O15245(Human)