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BOB1 Antibody (BOB1/2424)

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Western Blot: BOB1 Antibody (BOB1/2424) [NBP2-75752] - Analysis of Raji cell lysate using BOB1 Mouse Monoclonal Antibody (BOB1/2424).
Immunocytochemistry/ Immunofluorescence: BOB1 Antibody (BOB1/2424) [NBP2-75752] - Immunofluorescent staining of paraformaldehyde-fixed Raji cells using BOB1 Antibody (BOB1/2424).followed by goat anti-Mouse IgG ...read more
Immunohistochemistry-Paraffin: BOB1 Antibody (BOB1/2424) [NBP2-75752] - Formalin-fixed, paraffin-embedded human Spleen stained with BOB1 Mouse Monoclonal Antibody (BOB1/2424).
Flow Cytometry: BOB1 Antibody (BOB1/2424) [NBP2-75752] - Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells. BOB1 Antibody (BOB1/2424).; followed by goat anti-Mouse IgG-CF488 (blue) and Isotype Control (Red).
Protein Array: BOB1 Antibody (BOB1/2424) [NBP2-75752] - Analysis of Protein Array containing >19,000 full-length human proteins using BOB1 Antibody (BOB1/2424) Z- and S- Score: The Z-score represents the strength of a ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, MA
Clone
BOB1/2424
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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BOB1 Antibody (BOB1/2424) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-75753)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant fragment (around aa 148-255) of human BOB1 protein (exact sequence is proprietary) (Uniprot: Q16633)
Localization
Nuclear
Marker
B-Cell Marker
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
POU2AF1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for BOB1 Antibody (BOB1/2424)

  • B-cell-specific coactivator OBF-1
  • BOB-1
  • OBF-1
  • OBF1BOB1
  • OCAB
  • OCA-B
  • OCT-binding factor 1
  • POU class 2 associating factor 1
  • POU domain class 2, associating factor 1
  • POU domain class 2-associating factor 1
  • POU domain, class 2, associating factor 1

Background

BOB1 is a novel 35kDa protein designated Bob1 (B cell Oct binding protein 1) is a lymphocyte specific transcription co activator protein. B cell specific transcription is mediated by octamer motifs found in immunoglobulin heavy and light chain gene promoters and in some immunoglobulin enhancers. These sites are bound by the B cell specific Oct1 and Oct2 transcription factors, and the B cell restricted co activator BOB.1/OBF.1. This protein interacts only with the Oct1/2 proteins through sub domains in the POU domain of the Oct1/2 proteins, enhancing their transcriptional efficacy. Although having no intrinsic capacity for DNA binding, Bob1 associates tightly with the octomer motif in the presence of Oct1 and Oct2. Bob1 is expressed at highest levels in spleen and peripheral blood leukocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BOB1 Antibody (NBP2-75752) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for BOB1 Antibody (NBP2-75752)

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Blogs on BOB1.

Winter Protein Games
Get into the Winter Protein Games 2014! Check out the contenders competing including SKI, POLE, ICEBURG, SKT, TRAIL, WIN and BOB1. Learn more about each protein's function, gene name, molecular weight and family.View all the fun and games happening ...  Read full blog post.

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Bioinformatics

Gene Symbol POU2AF1
Uniprot