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SLC22A2/OCT2 Antibody (640438) [Unconjugated]

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SLC22A2?/OCT2 Antibody was detected in immersion fixed paraffin-embedded sections of human Kidney using Mouse Anti-Human SLC22A2/OCT2, Monoclonal Antibody (Catalog # MAB6547) at 20ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​
SLC22A2?/OCT2 Antibody was detected in immersion fixed paraffin-embedded sections of human Kidney using Mouse Anti-Human SLC22A2/OCT2, Monoclonal Antibody (Catalog # MAB6547) at 20ug/mL at 37 ° Celsius for 4 minutes. ...read more
SLC22A2 was detected in immersion fixed paraffin-embedded sections of human kidney using Human SLC22A2 Monoclonal Antibody (Catalog # MAB6547) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse ...read more
HEK293 human embryonic kidney cell line transfected with EGP and (A) human SLC22A2/OCT2 or (B) irrelevant protein was stained with Mouse Anti-Human SLC22A2/OCT2 Monoclonal Antibody (Catalog # MAB6547) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, mIF
Clone
640438
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB6547U-100UG
Alexa Fluor 405
FAB6547V-100UG
Alexa Fluor 488
FAB6547G-100UG
Alexa Fluor 532
FAB6547X
Alexa Fluor 594
FAB6547T-100UG
Alexa Fluor 647
FAB6547R-100UG
Alexa Fluor 700
FAB6547N-100UG
Alexa Fluor 750
FAB6547S-100UG
Allophycocyanin
FAB6547A
Allophycocyanin/Cy7
FAB6547APCCY7
Biotin
FAB6547B
CoraFluor 1
FAB6547CL1
DyLight 350
FAB6547D
DyLight 405
FAB6547E
DyLight 488
FAB6547K
DyLight 550
FAB6547L
DyLight 594
FAB6547M
DyLight 650
FAB6547W
DyLight 680
FAB6547Y
DyLight 755
FAB6547Z
FITC
FAB6547F
HRP
FAB6547H
Janelia Fluor 525
FAB6547JF525
Janelia Fluor 549
FAB6547I
Janelia Fluor 646
FAB6547J
Janelia Fluor 669
FAB6547JF669
PE
FAB6547P
PE/Atto594
FAB6547PEATT594
PE/Cy5.5
FAB6547PECY55
PE/Cy7
FAB6547PECY7
PerCP
FAB6547C
mFluor Violet 450 SE
FAB6547MFV450
mFluor Violet 500 SE
FAB6547MFV500
mFluor Violet 610 SE
FAB6547MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB6547T-100UG
Carrier Free
FAB6547R-100UG
Carrier Free
FAB6547G-100UG
Carrier Free
FAB6547V-100UG
Carrier Free
FAB6547U-100UG
Carrier Free
FAB6547N-100UG
Carrier Free
FAB6547S-100UG
Carrier Free
MAB6547-SP
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SLC22A2/OCT2 Antibody (640438) [Unconjugated] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human SLC-22A2

Accession # O15244
Specificity
Detects human SLC22A2 in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SLC22A2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Multiplex Immunofluorescence 20 ug/mL
Publications
Read Publications using
MAB6547 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SLC22A2/OCT2 Antibody (640438) [Unconjugated]

  • hOCT2
  • MGC32628
  • OCT2
  • OCT2Organic cation transporter 2
  • SLC22A2
  • solute carrier family 22 (organic cation transporter), member 2
  • solute carrier family 22 member 2

Background

Solute carrier family 22 member 2 (SLC22A2; also hOCT2) is a 65 kDa member of the major facilitator superfamily and organic cation transporter family of proteins.   Human SLC22A2 is synthesized as a multipass transmembrane protein that is 555 amino acids (aa) in length.  Human SLC22A2 contains one potential site for N-linked glycosylation.  There are also two additional isoforms for human SLC22A2.   Isoform 2 has a 57 aa substitution for aa 427-483 and a deletion of the 72 aa at positions 484-555.   Isoform 3 has an 18 aa substitution for aa 225-242 and a deletion of residues 243-555.  SLC22A2 has its highest expression in the kidney.  It is also expressed at lower levels in neurons of the cerebral cortex and in various subcortical nuclei.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SLC22A2/OCT2 Antibody (MAB6547)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Immunohistochemistry.


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Bioinformatics

Gene Symbol SLC22A2
Uniprot