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Podoplanin Antibody [Unconjugated]

 (74 Publications)
 (1 Review)
 
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Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3244) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Podoplanin at approximately 35-42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3244) followed by HRP-conjugated ...read more
Podoplanin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3244) at 15 µg/mL overnight at 4 °C. Tissue was ...read more
Simple Western shows lysates of mouse lung, loaded at 0.5 mg/ml. A specific band was detected for Podoplanin at approximately 60 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse Podoplanin Antigen Affinity-purified ...read more
Mfn1/2i delta AEC2 mice develop spontaneous lung fibrosis. a Schema demonstrating the generation of mice with AEC2 cell-specific tamoxifen-inducible deletion of Mfn1/2. SftpcCreERT2+/+ or SftpcCreERT2+/− mice were ...read more
Effect of VEGFCC156S on angiotensin II-induced cardiac dysfunction, cardiac lymphatics, and skin lymphatics.(A) Pharmacokinetic analysis of VEGFCC156S in mice (n = 2 per dose group). (B) Plasma VEGFC concentration ...read more
Carboplatin induces lymphangiogenesis in healthy tissues (A) Schematic of rat mesentery culture model. (B) Vehicle-treated lymphatic vessels from mesentery cultures stained with LYVE-1 (grey). (i) High magnification ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF3244
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Catalog# & Formulation Size Price
Carrier Free
AF3244-SP
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Podoplanin Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Podoplanin
Gln21-Lys133
Accession # Q546R8
Specificity
Detects mouse Podoplanin in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human Podoplanin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PDPN
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.25 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF3244 in the following applications:
Publications
Read Publications using
AF3244 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Podoplanin Antibody [Unconjugated]

  • 36-KD
  • Aggrus
  • Gp36
  • Gp38
  • GP40
  • HT1A-1
  • hT1alpha-1
  • hT1alpha-2
  • lung type I cell membrane associated glycoprotein
  • lung type-I cell membrane-associated glycoprotein (T1A-2)
  • OTS 8
  • OTS8
  • PA2.26 antigen
  • PDPN
  • Podoplanin
  • RANDAM-2
  • T1A
  • T1A-2
  • T1-alpha

Background

Podoplanin, also known as T1 alpha (T1 alpha ), is a mucin type transmembrane glycoprotein with extensive O-glycosylation. It is specifically expressed by lymphatic endothelial cells but not blood vascular endothelial cells. In addition, non-endothelial cells in numerous normal tissues also express the protein. Within the region used as the immunogen, mouse Podoplanin shares 73.5% and 29% amino acid sequence homology with rat and human Podoplanin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Podoplanin Antibody (AF3244)(74)

We have publications tested in 4 confirmed species: Human, Mouse, Canine, Transgenic Mouse.

We have publications tested in 10 applications: Flow Cytometry, ICC, IF, IHC, IHC-Fr, IHC-P, IHC/IF, Immunohistochemistry, Neutralization, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(2)
IF
(2)
IHC
(17)
IHC-Fr
(10)
IHC-P
(8)
IHC/IF
(1)
Immunohistochemistry
(1)
Neutralization
(1)
Western Blot
(5)
All Applications
Filter By Species
Human
(2)
Mouse
(44)
Canine
(1)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 74. Show All 74 Publications.
Publications using AF3244 Applications Species
Lauer, D;Magnin, CY;Kolly, LR;Wang, H;Brunner, M;Chabria, M;Cereghetti, GM;Gabry?, HS;Tanadini-Lang, S;Uldry, AC;Heller, M;Verleden, SE;Klein, K;Sarbu, AC;Funke-Chambour, M;Ebner, L;Distler, O;Maurer, B;Gote-Schniering, J; Radioproteomics stratifies molecular response to antifibrotic treatment in pulmonary fibrosis JCI insight 2024-07-16 [PMID: 39012714] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Fernandes, R;Barbosa-Matos, C;Borges-Pereira, C;Carvalho, ALRT;Costa, S; Glycogen Synthase Kinase-3 Inhibition by CHIR99021 Promotes Alveolar Epithelial Cell Proliferation and Lung Regeneration in the Lipopolysaccharide-Induced Acute Lung Injury Mouse Model International journal of molecular sciences 2024-01-20 [PMID: 38279281] (Western Blot, Mouse) Western Blot Mouse
Singla B, Aithbathula RV, Pervaiz N et al. CD47 Activation by Thrombospondin-1 in Lymphatic Endothelial Cells Suppresses Lymphangiogenesis and Promotes Atherosclerosis Arteriosclerosis, thrombosis, and vascular biology 7/1/2023 [PMID: 37259865]

Details:
IHC Dilution: 1:100
Xiao, Z;Todd, L;Huang, L;Noguera-Ortega, E;Lu, Z;Huang, L;Kopp, M;Li, Y;Pattada, N;Zhong, W;Guo, W;Scholler, J;Liousia, M;Assenmacher, CA;June, CH;Albelda, SM;Puré, E; Desmoplastic stroma restricts T cell extravasation and mediates immune exclusion and immunosuppression in solid tumors bioRxiv : the preprint server for biology 2023-08-07 [PMID: 37090547] (IHC/IF, Mouse) IHC/IF Mouse
J Suzuki, Y Shimizu, T Hayashi, Y Che, Z Pu, K Tsuzuki, S Narita, R Shibata, I Ishii, JW Calvert, T Murohara Hydrogen Sulfide Attenuates Lymphedema Via the Induction of Lymphangiogenesis Through a PI3K/Akt-Dependent Mechanism Journal of the American Heart Association, 2022-10-26;11(21):e026889. 2022-10-26 [PMID: 36285798] (IHC, Mouse) IHC Mouse
Q Li, R Wang, Z Zhang, H Wang, X Lu, J Zhang, AP Kong, XY Tian, HF Chan, AC Chung, JC Cheng, Q Jiang, WY Lee Sirt3 mediates the benefits of exercise on bone in aged mice Cell Death and Differentiation, 2022-09-24;0(0):. 2022-09-24 [PMID: 36153410] (IHC, Mouse) IHC Mouse
Yi Luan, Qianying Yuan, Qijun Wang, Susan Compton, Dianqing Wu, Wenwen Tang Pazopanib Is a Potential Treatment for Coronavirus-Induced Lung Injuries The Journal of Immunology 8/15/2022 [PMID: 35914834]
H Moriyama, J Endo, M Kataoka, Y Shimanaka, N Kono, Y Sugiura, S Goto, H Kitakata, T Hiraide, N Yoshida, S Isobe, T Yamamoto, K Shirakawa, A Anzai, Y Katsumata, M Suematsu, K Kosaki, K Fukuda, H Arai, M Sano Omega-3 fatty acid epoxides produced by PAF-AH2 in mast cells regulate pulmonary vascular remodeling Nature Communications, 2022-05-31;13(1):3013. 2022-05-31 [PMID: 35641514] (IHC, Mouse) IHC Mouse
Alexandra R. Harris, Savieay Esparza, Mohammad S. Azimi, Robert Cornelison, Francesca N. Azar, Danielle C. Llaneza et al. Platinum Chemotherapy Induces Lymphangiogenesis in Cancerous and Healthy Tissues That Can be Prevented With Adjuvant Anti-VEGFR3 Therapy Frontiers in Oncology 3/17/2022 [PMID: 35372032]
G Günes Güns, TM Conlon, A Jeridi, R Kim, Z Ertüz, NJ Lang, M Ansari, M Novikova, D Jiang, M Strunz, M Gaianova, C Hollauer, C Gabriel, I Angelidis, S Doll, JC Pestoni, SL Edelmann, MS Kohlhepp, A Guillot, K Bassler, HP Van Eeckho, Ö Kayalar, N Konyalilar, T Kanashova, S Rodius, C Ballester-, CM Genes Robl, N Smirnova, M Rehberg, C Agarwal, I Krikki, B Piavaux, SE Verleden, B Vanaudenae, M Königshoff, G Dittmar, KR Bracke, JL Schultze, H Watz, O Eickelberg, T Stoeger, G Burgstalle, F Tacke, V Heissmeyer, Y Rinkevich, H Bayram, HB Schiller, M Conrad, R Schneider, AÖ Yildirim The arginine methyltransferase PRMT7 promotes extravasation of monocytes resulting in tissue injury in COPD Nature Communications, 2022-03-14;13(1):1303. 3/14/2022 [PMID: 35288557]
Show All 74 Publications.

Review for Podoplanin Antibody (AF3244) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF3244:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
 ICC Mouse 01/07/2015
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Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 23892627
SpeciesMouse

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By Jennifer Sokolowski, MD, PhD. Identification and characterization of meningeal lymphaticsThe recent discovery of a lymphatic system in the meninges surrounding the brain and spinal cord has spurred a surge of int...  Read full blog post.
Podoplanin (OST8, Glycoprotein (Gp) 36 or 38, Lung Type I Cell Membrane Associated Glycoprotein)
Podoplanin is a mucin-type 1 transmembrane glycoprotein found in a wide range of tissues. It appears to be differentially expressed in endothelial cells of lymphatic but not blood vessel origin. In normal skin and kidney, podoplanin co-localizes with ...  Read full blog post.
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Application: ICC
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