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LYVE-1 Antibody [Unconjugated]

 (141 Publications)
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LYVE-1 was detected in perfusion fixed frozen sections of mouse liver using 15 µg/mL Goat Anti-Mouse LYVE-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2125) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of endothelial cells in sinusoids. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
LYVE-1 was detected in perfusion fixed frozen sections of mouse liver using 15 µg/mL Goat Anti-Mouse LYVE-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2125) overnight at 4 °C. Tissue was stained with ...read more
Western blot shows lysates of mouse liver tissue and bEnd.3 mouse brain endothelial cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse LYVE-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
17D-204 causes viscerotropic abnormalities in the absence of IFN-gamma . H&E sections (a–c) antibody-stained frozen sections (b, d) of spleen (a, b) and liver (c, d) were presented. a Spleen sections from ...read more
Molecular characterization of LECs in the SCS ceiling with RNA FISH.(A-B) Expression of new cLEC/cluster 2 marker genes Ackr3 (A) and Btnl9 (B) by RNA sequencing (left panels) and RNA FISH (right panels). As GFP ...read more
Molecular characterization of LECs in the SCS ceiling with immunofluorescence staining.(A–C) Expression of new cLEC/cluster 2 marker genes ANXA2 (A), FABP4 (B), and CD36 (C) by RNA sequencing (left panels) and ...read more
Molecular characterization of LECs in the SCS ceiling with RNA FISH.(A-B) Expression of new cLEC/cluster 2 marker genes Ackr3 (A) and Btnl9 (B) by RNA sequencing (left panels) and RNA FISH (right panels). As GFP ...read more
POSTN is upregulated in (pre)-metastatic LN. Morphometric analysis of POSTN and LYVE1+ lymphatic vessels in experimental (pre)-metastatic LN (in the ear sponge assay using B16F10 cells). CTRLs correspond to mice ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, Dual ISH-IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF2125
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Catalog# & Formulation Size Price
Carrier Free
AF2125-SP
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LYVE-1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse LYVE-1
Ala24-Thr234
Accession # Q8BHC0
Specificity
Detects mouse LYVE-1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LYVE1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Dual RNAscope ISH-IHC 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.25 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF2125 in the following applications:
Publications
Read Publications using
AF2125 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LYVE-1 Antibody [Unconjugated]

  • cell surface retention sequence binding protein-1
  • Cell surface retention sequence-binding protein 1
  • CRSBP1
  • CRSBP-1
  • extracellular link domain containing 1
  • extracellular link domain-containing 1
  • Extracellular link domain-containing protein 1
  • HAR
  • Hyaluronic acid receptor
  • lymphatic vessel endothelial hyaluronan receptor 1
  • lymphatic vessel endothelial hyaluronic acid receptor 1
  • LYVE1
  • LYVE-1
  • UNQ230/PRO263
  • XLKD1

Background

Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a recently identified receptor of HA, a linear high molecular weight polymer composed of alternating units of D-glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease. The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively. LYVE-1 shares 41% homology with the other known HA receptor, CD44. The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican. Human and mouse LYVE-1 share 69% amino acid sequence identity.

LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels. In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells. LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes. Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LYVE-1 Antibody (AF2125)(141)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Porcine, Transgenic Mouse.

We have publications tested in 13 applications: Cell Selection, Flow Cytometry, ICC, ICC/IF, IF, IF/IHC, IHC, IHC-Fr, IHC-P, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot.


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Cell Selection
(1)
Flow Cytometry
(2)
ICC
(5)
ICC/IF
(1)
IF
(1)
IF/IHC
(1)
IHC
(54)
IHC-Fr
(7)
IHC-P
(9)
Immunofluorescence
(1)
Immunohistochemistry
(7)
Immunoprecipitation
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(5)
Mouse
(79)
Rat
(1)
Porcine
(1)
Transgenic Mouse
(6)
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Showing Publications 1 - 10 of 141. Show All 141 Publications.
Publications using AF2125 Applications Species
Igarashi, A;Hayashi, T;Shimizu, T;Yuda, K;Yamagami, S; Inhibiting corneal transplantation rejection via lymphatic vessel ligation in a novel murine model Scientific reports 2024-10-28 [PMID: 39465339] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Zhao, D;Huang, Z;Li, X;Wang, H;Hou, Q;Wang, Y;Yan, F;Yang, W;Liu, D;Yi, S;Han, C;Hao, Y;Tang, L; GDF2 and BMP10 coordinate liver cellular crosstalk to maintain liver health eLife 2024-10-25 [PMID: 39453386] (Immunohistochemistry, Transgenic Mouse) Immunohistochemistry Transgenic Mouse
Rosmus, DD;Koch, J;Hausmann, A;Chiot, A;Arnhold, F;Masuda, T;Kierdorf, K;Hansen, SM;Kuhrt, H;Fröba, J;Wolf, J;Boneva, S;Gericke, M;Ajami, B;Prinz, M;Lange, C;Wieghofer, P; Redefining the ontogeny of hyalocytes as yolk sac-derived tissue-resident macrophages of the vitreous body Journal of neuroinflammation 2024-07-03 [PMID: 38961498] (Immunofluorescence, Mouse) Immunofluorescence Mouse
Felgendreff, P;Hosseiniasl, SM;Minshew, A;Amiot, BP;Wilken, S;Ahmadzada, B;Huebert, RC;Sakrikar, NJ;Engles, NG;Halsten, P;Mariakis, K;Barry, J;Riesgraf, S;Fecteau, C;Ross, JJ;Nyberg, SL; First Application of a Mixed Porcine-Human Repopulated Bioengineered Liver in a Preclinical Model of Post-Resection Liver Failure Biomedicines 2024-06-07 [PMID: 38927479] (Immunohistochemistry, Human, Porcine) Immunohistochemistry Human, Porcine
Sorets, AG;Schwensen, KR;Francini, N;Kjar, A;Abdulrahman, AM;Shostak, A;Katdare, KA;Schoch, KM;Cowell, RP;Park, JC;Ligocki, AP;Ford, WT;Ventura-Antunes, L;Hoogenboezem, EN;Prusky, A;Castleberry, M;Michell, DL;Miller, TM;Vickers, KC;Schrag, MS;Duvall, CL;Lippmann, ES; Lipid-siRNA conjugate accesses a perivascular transport mechanism and achieves widespread and durable knockdown in the central nervous system bioRxiv : the preprint server for biology 2024-06-10 [PMID: 38915549] (Immunohistochemistry, Rat) Immunohistochemistry Rat
Gan, C;Yaqoob, U;Lu, J;Xie, M;Anwar, AA;Jalan-Sakrikar, N;Jerez, S;Sehrawat, T;Navarro-Corcuera, A;Kostallari, E;Habash, NW;Cao, S;Shah, VH; Liver Sinusoidal Endothelial Cells Contribute to Portal Hypertension Through Collagen Type IV-Driven Sinusoidal Remodeling JCI insight 2024-05-07 [PMID: 38713515] (Immunohistochemistry, Transgenic Mouse) Immunohistochemistry Transgenic Mouse
Vishlaghi, N;Guo, L;Griswold-Wheeler, D;Sun, Y;Booker, C;Crossley, JL;Bancroft, AC;Juan, C;Korlakunta, S;Ramesh, S;Pagani, CA;Xu, L;James, AW;Tower, RJ;Dellinger, M;Levi, B; Vegfc-expressing cells form heterotopic bone after musculoskeletal injury Cell reports 2024-04-03 [PMID: 38573853] (Immunohistochemistry, Mouse, Transgenic Mouse) Immunohistochemistry Mouse, Transgenic Mouse
Creff, J;Lamaa, A;Benuzzi, E;Balzan, E;Pujol, F;Draia-Nicolau, T;Nougué, M;Verdu, L;Morfoisse, F;Lacazette, E;Valet, P;Chaput, B;Gross, F;Gayon, R;Bouillé, P;Malloizel-Delaunay, J;Bura-Rivière, A;Prats, AC;Garmy-Susini, B; Apelin-VEGF-C mRNA delivery as therapeutic for the treatment of secondary lymphedema EMBO molecular medicine 2024-01-02 [PMID: 38177539] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Zamora, A;Nougué, M;Verdu, L;Balzan, E;Draia-Nicolau, T;Benuzzi, E;Pujol, F;Baillif, V;Lacazette, E;Morfoisse, F;Galitzky, J;Bouloumié, A;Dubourdeau, M;Chaput, B;Fazilleau, N;Malloizel-Delaunay, J;Bura-Rivière, A;Prats, AC;Garmy-Susini, B; 15-Lipoxygenase promotes resolution of inflammation in lymphedema by controlling Treg cell function through IFN-? Nature communications 2024-01-04 [PMID: 38177096] (IF/IHC, Mouse) IF/IHC Mouse
Rouaud, L;Baudin, L;Gautier-Isola, M;Van Meerbeeck, P;Feyereisen, E;Blacher, S;van Baren, N;Kridelka, F;Lucas, S;Noel, A; Spatial Distribution of Non-Immune Cells Expressing Glycoprotein A Repetitions Predominant in Human and Murine Metastatic Lymph Nodes Cancers 2023-11-28 [PMID: 38067325] (IHC, Mouse) IHC Mouse
Show All 141 Publications.

Review for LYVE-1 Antibody (AF2125) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF2125:
Filter by Applications
Flow
(1)
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Mouse
(1)
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  4
reviewed by:
Verified Customer
 Flow Mouse 01/06/2015
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Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 22287283
SpeciesMouse

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Blogs on LYVE-1.

Check out the latest blog posts on LYVE-1.
Meningeal lymphatics: recent discovery defying the concept of central nervous system 'immune privilege'
By Jennifer Sokolowski, MD, PhD. Identification and characterization of meningeal lymphaticsThe recent discovery of a lymphatic system in the meninges surrounding the brain and spinal cord has spurred a surge of int...  Read full blog post.
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 Verified Customer
01/06/2015
Application: Flow
Species: Mouse
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Bioinformatics

Gene Symbol LYVE1
Uniprot