CLEC-2/CLEC-1B Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

C-type lectin-like receptor 2 (CLEC-2) is a 32 kDa type II transmembrane glycoprotein and member of the C-type lectin-like family of receptors (1-4). CLEC-2 consists of a 33 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane region, and a 175 aa extracellular domain. The cytoplasmic domain contains multiple threonine and serine residues which are sites of potential phosphorylation, and a YXXL (Tyr-Xaa-Xaa-Leu) motif through which CLEC-2 does its signaling (2, 4-5). Ligand binding and cross-linking of CLEC-2 induces Src kinase-dependent tyrosine phosphorylation of the YXXL sequence, inducing activation of the tyrosine kinase Syk and initiation of a signaling pathway that culminates in activation of phospholipase C gamma 2 (2, 5). The extracellular domain contains three potential sites of N-linked glycosylation, and a single carbohydrate recognition domain (CRD) which shows conservation of six cysteine residues (1, 6). Unlike most other members of the
C‑type lectin-like family of receptors, CLEC-2's CRD lacks the amino acid residues that are crucial for Ca²⁺-dependent carbohydrate binding, making it a
non‑classical C-type lectin receptor (1, 6). A splicing variant at aa 22-55 produces two isoforms for CLEC-2. Isoform 1 is the longer protein, and in isoform 2, an alanine residue is substituted for aa 22-55. Human CLEC-2 shares 63% aa sequence identity with mouse CLEC-2. CLEC-2 is expressed preferentially in liver, and is also detected in myeloid cells (monocytes, dendritic cells, and granulocytes) (1), platelets, and megakaryocytes (4). CLEC-2 is the receptor for the
platelet‑aggregating snake venom protein rhodocytin (3-4) and the molecule podoplanin, a transmembrane sialoglycoprotein that, when bound to CLEC-2, is involved in platelet aggregation, tumor metastasis, and lymphatic vessel formation (2, 7). CLEC-2 has also been shown to enhance infectivity of HIV-1 by mediating HIV-1 attachment and transfer by CLEC-2 transfected cells and platelets (8).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: Functional Assay, ICC, IHC, IHC-P, Immunoprecipitation, Neutralization, Western Blot.

Showing Publications 1 - 8 of 8.

Publications using AF1718	Applications	Species
Luis A. Morán, Ying Di, Marcin A. Sowa, Lidia Hermida-Nogueira, María N. Barrachina, Eleyna Martin et al. Katacine Is a New Ligand of CLEC-2 that Acts as a Platelet Agonist Thrombosis and Haemostasis 8/1/2022 [PMID: 35170009]
Yifan Wu, Zhimin Zeng, Yubiao Guo, Lizhen Song, Jill E. Weatherhead, Xinyan Huang et al. Candida albicans elicits protective allergic responses via platelet mediated T helper 2 and T helper 17 cell polarization Immunity 11/1/2021 [PMID: 34506733]
Nobuo Watanabe, Masako Kidokoro, Yusuke Suzuki, Makiko Tanaka, Shigeaki Inoue, Hideo Tsukamoto et al. A pull-down and slot blot-based screening system for inhibitor compounds of the podoplanin-CLEC-2 interaction PLOS ONE 9/25/2019 [PMID: 31553741]
A Milasan, G Jean, F Dallaire, JC Tardif, Y Merhi, M Sorci-Thom, C Martel Apolipoprotein A-I Modulates Atherosclerosis Through Lymphatic Vessel-Dependent Mechanisms in Mice J Am Heart Assoc, 2017-09-22;6(9):. 2017-09-22 [PMID: 28939717] (Western Blot, Mouse)	Western Blot	Mouse
C Delierneux, N Donis, L Servais, O Wéra, C Lecut, M Vandereyke, L Musumeci, S Rahmouni, J Schneider, JA Eble, P Lancellott, C Oury Targeting of C-type lectin-like receptor 2 or P2Y12 for the prevention of platelet activation by immunotherapeutic CpG oligodeoxynucleotides J. Thromb. Haemost, 2017-04-13;0(0):. 2017-04-13 [PMID: 28296036] (Functional Assay, Immunoprecipitation, Western Blot, Human, Mouse)	Functional Assay, Immunoprecipitation, Western Blot	Human, Mouse
Del Rey M, Fare R, Izquierdo E, Usategui A, Rodriguez-Fernandez J, Suarez-Fueyo A, Canete J, Pablos J Clinicopathological correlations of podoplanin (gp38) expression in rheumatoid synovium and its potential contribution to fibroblast platelet crosstalk. PLoS ONE, 2014-06-16;9(6):e99607. 2014-06-16 [PMID: 24932813] (IHC, Western Blot, Human)	IHC, Western Blot	Human
Fuller GL, Williams JA, Tomlinson MG, Eble JA, Hanna SL, Pohlmann S, Suzuki-Inoue K, Ozaki Y, Watson SP, Pearce AC The C-type lectin receptors CLEC-2 and Dectin-1, but not DC-SIGN, signal via a novel YXXL-dependent signaling cascade. J. Biol. Chem., 2007-03-05;282(17):12397-409. 2007-03-05 [PMID: 17339324] (Functional Assay, Human)	Functional Assay	Human
Chaipan C, Soilleux EJ, Simpson P, Hofmann H, Gramberg T, Marzi A, Geier M, Stewart EA, Eisemann J, Steinkasserer A, Suzuki-Inoue K, Fuller GL, Pearce AC, Watson SP, Hoxie JA, Baribaud F, Pohlmann S DC-SIGN and CLEC-2 mediate human immunodeficiency virus type 1 capture by platelets. J. Virol., 2006-09-01;80(18):8951-60. 2006-09-01 [PMID: 16940507] (ICC, IHC-P, Neutralization, Human)	ICC, IHC-P, Neutralization	Human

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Verified Customer	IHC-P	Human	10/05/2020	View Summary Application Immunohistochemistry-Paraffin Sample Tested FFPE Species Human Lot KSC012007A

Array AF1718

• CLEC-2/CLEC-1B Antibodies
• CLEC-2/CLEC-1B Proteins and Enzymes