Reactivity | HuSpecies Glossary |
Applications | ELISA, ICC/IF, IHC |
Clone | PDPN/4009R |
Clonality | Monoclonal |
Host | Rabbit |
Conjugate | Unconjugated |
Concentration | 0.2 mg/ml |
Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08816) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
Additional Information | Recombinant Monoclonal Antibody |
Immunogen | Synthetic peptide corresponding to residues within aa1-100 of Podoplanin (Uniprot: Q86YL7) |
Localization | Cell Surface |
Marker | Lymphatic Endothelial & Mesothelial Marker |
Isotype | IgG |
Clonality | Monoclonal |
Host | Rabbit |
Gene | PDPN |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | ELISA: For coating, order antibody without BSA). Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
Theoretical MW | 17 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Storage | Store at 4C. |
Buffer | 10 mM PBS with 0.05% BSA |
Preservative | 0.05% Sodium Azide |
Concentration | 0.2 mg/ml |
Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Podoplanin Antibody (NBP3-07494)Find related products by research area.
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Podoplanin (OST8, Glycoprotein (Gp) 36 or 38, Lung Type I Cell Membrane Associated Glycoprotein) Podoplanin is a mucin-type 1 transmembrane glycoprotein found in a wide range of tissues. It appears to be differentially expressed in endothelial cells of lymphatic but not blood vessel origin. In normal skin and kidney, podoplanin co-localizes with ... Read full blog post. |
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