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PNMT Antibody

Images

 
Western Blot: PNMT Antibody [NBP2-19877] - Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: PNMT Antibody [NBP2-19877] - Analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.
Western Blot: PNMT Antibody [NBP2-19877] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PNMT antibody [N2C3] (NBP2-19877) diluted at 1:1000. The HRP-conjugated ...read more
Immunohistochemistry-Paraffin: PNMT Antibody [NBP2-19877] - PNMT antibody [N2C3] detects PNMT protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse placenta.PNMT stained by PNMT antibody ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PNMT Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PNMT. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PNMT
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Porcine (89%), Rat (86%), Bovine (89%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for PNMT Antibody

  • EC 2.1.1
  • EC 2.1.1.28
  • Noradrenaline N-methyltransferase
  • PENT
  • PENTMGC34570
  • phenylethanolamine N-methylase
  • phenylethanolamine N-methyltransferase
  • PNMT
  • PNMTase

Background

The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PNMT
Uniprot