| Reactivity | Hu, MuSpecies Glossary |
| Applications | IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | E. coli-derived recombinant human PIWIL1/HIWI Arg82-Thr290 Accession # Q96J94 |
| Specificity | Detects human PIWIL1/HIWI in direct ELISAs. |
| Source | N/A |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | PIWIL1 |
| Purity Statement | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Sterile PBS to a final concentration of 0.2 mg/mL. |
PIWI (P-element-induced wimpy testis; also HIWI and PIWIL1) is a 92-98 kDa member of the Piwi subfamily, Argonaute family of proteins. It is expressed in germline cells, particularly spermatocytes and spermatids, and also appears in CD34+ hematopoietic stem cells plus select tumor types. PIWI binds to a series of 28‑34 nucleic acid long noncoding RNAs that participate in gametogenesis. It does so under the influence of Arg methyltransferases, and in conjunction with Tudor‑containing proteins. Human PIWI/PIWIL1 is 861 amino acids (aa) in length. It contains a methylation target region in the N-terminus, followed by an RNA-binding PAZ domain (aa 277-391) and an RNA-cleaving PIWI domain (aa 555-847). There are two isoform variants. One is widely expressed and shows a three aa substitution for aa 1‑89. The second contains a six aa substitution for aa 824-861. Over aa 82-290, human PIWI (HIWI) shares 95% aa sequence identity with mouse PIWI (MIWI).
Secondary Antibodies |
Isotype Controls |
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