PIASy Antibody

The interaction of a cytokine with its specific cell surface receptor triggers the activation of intracellular signaling pathways that ultimately program the cellular response. The signal transducers and activators of transcription (STAT) proteins play important roles in the activation of these pathways. Recently, a family of proteins has been identified that inhibits activated STATS and are named as Protein Inhibitors of Activated STAT (PIAS). PIAS proteins comprise a family of five mammalian proteins, which have been identified as potentially important down regulators of the STAT-signaling pathway. The cDNA for mouse and human PIASg encodes a protein of 507 amino acids that is highly homologous to the human protein and is expressed in the mouse as early as d 7.5 of gestation. In situ hybridizations of staged mouse embryos localized the transcript for the PIASg gene to the limbs, neuroepithelium, and the inner root sheath of the hair follicle, suggesting a role in the development of these structures. This protein is also present in hair follicle of human scalp hair and in monkey neuronal cells. Thus PIASg exhibits a highly selective pattern of expression, suggesting that it modulates the response of cells to developmental cues.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Trypanosoma brucei.

We have publications tested in 2 applications: ICC/IF, WB.

Showing Publications 1 - 7 of 7.

Publications using NB600-1318	Applications	Species
Robert Siddaway, Scott Milos, Étienne Coyaud, Hwa Young Yun, Shahir M. Morcos, Sanja Pajovic, Eric I. Campos, Brian Raught, Cynthia Hawkins The in v ivo Interaction Landscape of Histones H3.1 and H3.3 Molecular & Cellular Proteomics : MCP 2022-09-09 [PMID: 36089195]
Tong Hua, Shen Rong, Wang ZhuMing et al. DNA ploidy cytometry testing for cervical cancer screening in China (DNACIC Trial): a prospective randomized, controlled trial. Clin Cancer Res. 2009-10-15 [PMID: 19825960] (ICC/IF, Human)	ICC/IF	Human
La Salle S, Sun F, Zhang XD et al. Developmental control of sumoylation pathway proteins in mouse male germ cells. Dev Biol. 2008-09-01 [PMID: 18602382] (WB) Details: SAE2 (IMG-5111A), PIASg (IMG-290), SENP1 CT (IMG-521). WB: Male germ cells; leptotene/zygotene, prepubertal pachytene, and adult pachytene spermatocytes, Fig 5.	WB
Brayman MJ, Dharmaraj N, Lagow E et al. MUC1 expression is repressed by protein inhibitor of activated signal transducer and activator of transcription-y. Mol Endocrinol. 2007-11-01 [PMID: 17717071] (ICC/IF, Human) Details: IF/ICC (confocal microscopy): Fig 8B, F [HEC-1A cells stably transfected with human progesterone receptor B (PRB) and left untreated or treated with progesterone].	ICC/IF	Human
Albor A, El-Hizawi S, Horn EJ et al. The interaction of Piasy with Trim32, an E3-ubiquitin ligase mutated in limb-girdle muscular dystrophy type 2H, promotes Piasy degradation and regulates UVB-induced keratinocyte apoptosis through NFkappaB. J Biol Chem. 2006-09-01 [PMID: 16816390] (ICC/IF, Trypanosoma brucei) Details: IF/ICC (confocal microscopy): Fig 5 (primary human dermal fibroblasts).	ICC/IF	Trypanosoma brucei
Ihara M, Yamamoto H, Kikuchi A. SUMO-1 modification of PIASy, an E3 ligase, is necessary for PIASy-dependent activation of Tcf-4. Mol Cell Biol. 2005-05-01 [PMID: 15831457] (WB, ICC/IF) Details: WB: Fg 1F (HeLa cell lysates immunoprecipitatated with His6-SUMO-1). IF/ICC (confocal micrcoscopy): Fig 3Aa (COS cells).	WB, ICC/IF
Zoumpoulidou G, Jones MC, Fernandez de Mattos S et al. Convergence of interferon-gamma and progesterone signaling pathways in human endometrium: role of PIASy (protein inhibitor of activated signal transducer and activator of transcription-y). Mol Endocrinol. 2004-08-01 [PMID: 15155784] (ICC/IF, WB, Human) Details: WB: Fig 5A [primary human endometrial stromal cells (ESCs)]; IF/ICC (confocal microscopy): Fig 6C (PIASy or progesterone transfected ESCs).	ICC/IF, WB	Human

Images	Ratings	Applications	Species	Date	Details
Enlarge	3 reviewed by: Verified Customer	WB	Human	10/02/2014	View Summary Application Western Blot Sample Tested Jurkat cells Species Human

Array NB600-1318

• PIASy Antibodies
• PIASy Lysate
• PIASy Protein