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TRIM32 Antibody (2E5)

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Western Blot: TRIM32 Antibody (2E5) [H00022954-M09] - Analysis of TRIM32 expression in transfected 293T cell line by TRIM32 monoclonal antibody (M09), clone 2E5. Lane 1: TRIM32 transfected lysatE (72 KDa). Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: TRIM32 Antibody (2E5) [H00022954-M09] - Analysis of monoclonal antibody to TRIM32 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: TRIM32 Antibody (2E5) [H00022954-M09] - Analysis of TRIM32 over-expressed 293 cell line, cotransfected with TRIM32 Validated Chimera RNAi or non-transfected control. Blot probed with H00022954-M09. GAPDH ...read more
ELISA: TRIM32 Antibody (2E5) [H00022954-M09] - Detection limit for recombinant GST tagged TRIM32 is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, KD
Clone
2E5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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TRIM32 Antibody (2E5) Summary

Immunogen
TRIM32 (NP_036342, 105 a.a. ~ 204 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RRLPRQFCRSCGLVLCEPCREADHQPPGHCTLPVKEAAEERRRDFGEKLTRLRELMGELQRRKAALEGVSKDLQARYKAVLQEYGHEERRVQDELARSRK
Specificity
TRIM32 (2E5)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TRIM32
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Knockdown Validated
  • Western Blot 1:500
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA.
Publications
Read Publications using
H00022954-M09 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TRIM32 Antibody (2E5)

  • 72 kDa Tat-interacting protein
  • BBS11
  • BBS1172-KD
  • EC 6.3.2.-
  • HT2A
  • HT2Alimb girdle muscular dystrophy 2H (autosomal recessive)
  • LGMD2H
  • TATIP
  • TRIM32
  • tripartite motif containing 32
  • Zinc finger protein HT2A
  • zinc-finger protein HT2A

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00022954-M09
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, KD

Publications for TRIM32 Antibody (H00022954-M09)(10)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(1)
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Filter By Species
Human
(1)
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Showing Publications 1 - 10 of 10.
Publications using H00022954-M09 Applications Species
Liu J, Lu S, Zheng L et al. ATM-CHK2-TRIM32 axis regulates ATG7 ubiquitination to initiate autophagy under oxidative stress Cell reports 2023-11-28 [PMID: 37943659]
Nicklas S, Hillje AL, Okawa S et al. A complex of the ubiquitin ligase TRIM32 and the deubiquitinase USP7 balances the level of c-Myc ubiquitination and thereby determines neural stem cell fate specification. Cell Death Differ 2018-06-13 [PMID: 29899379]
Assereto S, Piccirillo R, Baratto S et al. The ubiquitin ligase tripartite-motif-protein 32 is induced in Duchenne muscular dystrophy. Lab Invest 2016-06-13 [PMID: 27295345]
Bahnassawy L, Perumal TM, Gonzalez-Cano L et al. TRIM32 modulates pluripotency entry and exit by directly regulating Oct4 stability. Sci Rep. 2015-08-26 [PMID: 26307407]
Lee ES, Kim WR, Kim Y et al. Polarized and Stage-Dependent Distribution of Immunoreactivity for Novel PDZ-Binding Protein Preso1 in Adult Neurogenic Regions. Endocrinol Metab (Seoul). 2014-09-25 [PMID: 25309794]
Izumi H, Kaneko Y. Trim32 facilitates degradation of MYCN on spindle poles and induces asymmetric cell division in human neuroblastoma cells. Cancer Res 2014-10-01 [PMID: 25100564] (ICC/IF, Human) ICC/IF Human
Nicklas S, Otto A, Wu X et al. TRIM32 Regulates Skeletal Muscle Stem Cell Differentiation and Is Necessary for Normal Adult Muscle Regeneration. PLoS One. 2012-01-27 [PMID: 22299041]
Zhang Y, Liu J, Yao S et al. Nuclear factor kappa B signaling initiates early differentiation of neural stem cells. Stem Cells. 2012-03-01 [PMID: 22134901]
Sato T, Okumura F, Kano S et al. TRIM32 promotes neural differentiation through retinoic acid receptor-mediated transcription. J Cell Sci. 2011-10-07 [PMID: 21984809]
Schwamborn JC, Berezikov E, Knoblich JA et al. The TRIM-NHL Protein TRIM32 Activates MicroRNAs Prevents Self-Renewal in Mouse . Neural Progenitors Cell;136(5):913-25. 2009-03-06 [PMID: 19269368]

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol TRIM32
Entrez
OMIM
Uniprot