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PIAS2 Antibody

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Western Blot: PIAS2 Antibody [NBP1-52022] - WB analysis of PIAS2 using anti-PIAS2 antibody on Jurkat cell lyste (30 ug cell lysate boiled in lysis buffer). Image from verified customer review.
Western Blot: PIAS2 Antibody [NBP1-52022] - Western blot analysis of PIAS2 (aa185-196) in Human Peripheral Lymphocytes lysate (35ug protein in RIPA buffer) using NBP1-52022 at 1ug/ml. Primary incubation was 1 hour. ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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PIAS2 Antibody Summary

Immunogen
Peptide with sequence PQQVREICISRD corresponding to internal region according to NP_004662.2.
Epitope
PQQVREICISRD
Specificity
This antibody is expected to recognize both reported isoforms (NP_775298.1; NP_004662.2).
Predicted Species
Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PIAS2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in human spleen and peripheral mononucleocytes lysates (calculated MW of 63.4 kDa band according to NP_775298.1).
Reviewed Applications
Read 1 Review rated 4
using
NBP1-52022 in the following applications:

Publications
Read Publication using NBP1-52022.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PIAS2 Antibody

  • Androgen receptor-interacting protein 3
  • ARIP3
  • DAB2-interacting protein
  • DIP
  • E3 SUMO-protein ligase PIAS2
  • MGC102682
  • miz
  • MIZ1
  • Msx-interacting zinc finger protein
  • Msx-interacting-zinc finger
  • PIAS2
  • PIAS-NY protein
  • PIASx alpha
  • PIASX
  • PIASX-ALPHA
  • PIASX-BETA
  • Protein inhibitor of activated STAT x
  • protein inhibitor of activated STAT, 2
  • Protein inhibitor of activated STAT2
  • SIZ2
  • zinc finger, MIZ-type containing 4
  • ZMIZ4

Background

This gene encodes a protein involved in the regulation of transcription factors involved in MAP kinase signaling. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1. Two alternatively spliced transcripts encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA

Publications for PIAS2 Antibody (NBP1-52022)(1)

Review for PIAS2 Antibody (NBP1-52022) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-52022:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot PIAS2 NBP1-52022
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4
reviewed by:
Verified Customer
WB Human 10/02/2014
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Summary

ApplicationWestern Blot
Sample TestedJurkat cells
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PIAS2 Antibody (NBP1-52022) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for PIAS2 Antibody (NBP1-52022)

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Blogs on PIAS2

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Recent Reviews

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4
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1
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Verified Customer
10/02/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol PIAS2
Entrez
Uniprot