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Periostin/OSF-2 Antibody [Unconjugated]

 (5 Publications)
 (1 Review)
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Human Periostin/OSF-2 was immunoprecipitated from human milk samples diluted in 1X Sample Diluent Concentrate 2 (DYC002) and incubated with 3 µg Goat Anti-Human Periostin/OSF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3548) or Normal Goat IgG Control (AB-108-C) plus 30 µL Protein G beads overnight. Immunoprecipitated Periostin/OSF-2 was detected by Western blot under reducing conditions using 1 µg/mL Goat Anti-Human Periostin/OSF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3548). View our recommended buffer recipes for immunoprecipitation.
Human Periostin/OSF-2 was immunoprecipitated from human milk samples diluted in 1X Sample Diluent Concentrate 2 (DYC002) and incubated with 3 µg Goat Anti-Human Periostin/OSF-2 Antigen Affinity-purified Polyclonal ...read more
Periostin/OSF-2 was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human Periostin/OSF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3548) at 10 µg/mL overnight at ...read more
Western blot shows lysates of Human Breast Cancer. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Periostin/OSF‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3548) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF3548
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Catalog# & Formulation Size Price
Carrier Free
AF3548-SP
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Periostin/OSF-2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Periostin
Asn22-Gln836
Accession # Q15063
Specificity
Detects human Periostin/OSF-2 in direct ELISAs and Western blots. In direct ELISAs, less than 40% cross‑reactivity with recombinant mouse Periostin and recombinant rat Periostin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
POSTN
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF3548 in the following applications:
Publications
Read Publications using
AF3548 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Periostin/OSF-2 Antibody [Unconjugated]

  • Fasciclin I-like
  • MGC119510
  • MGC119511
  • OSF2
  • OSF-2
  • OSF-2osteoblast specific factor 2 (fasciclin I-like)
  • OSF2periodontal ligament-specific periostin
  • Osteoblast-specific factor 2
  • PDLPOSTN
  • periostin isoform thy2
  • periostin isoform thy4
  • periostin isoform thy6
  • periostin isoform thy8
  • Periostin
  • periostin, osteoblast specific factor
  • PNRP11-412K4.1
  • POSTN
  • TRIF52

Background

Human OSF-2 (Osteoblast-Specific Factor 2), also known as Periostin, is a 170 kDa secreted homodimeric protein that belongs to the periostin family of the FAS1 superfamily of molecules. It is a TGF-beta inducible molecule that serves as both an adhesion molecule and tumor suppressor. It is synthesized as an 836 amino acid (aa) precursor that contains a 21 aa signal sequence and an 815 aa mature region. It is unknown if the molecule has any significant glycosylation. The human homodimer is not disulfide-linked. The molecule consists of two distinct regions. The N-terminus contains an 55 aa EMI domain, while the C-terminus contains four 130 aa Fasciculin type 1 (FAS1) domains. The EMI domain is cysteine-rich and shows a highly basic alpha -helix. Each FAS1 repeat exhibits a novel seven-stranded beta -wedge with a multiple alpha -helical fold. Three alternate splice forms are known that are C-terminal to the fourfold FAS1 repeats. These mature molecules are 758 and 761 aa in length. The first shows a one aa substitution for aa 649-706 of the mature molecule. The second shows a one aa substitution for aa 649-676, and a deletion of 27 aa between aa 784-810 of the mature molecule. The significance of the alternate splice forms is not clear. OSF-2 is known to bind to alpha v beta 3 and alpha v beta 5 integrins. It is synthesized by smooth muscle cells, fibroblasts, osteoblasts, and multiple carcinoma cell types. OSF-2 induces expression of VEGFR2/KDR on endothelial cells (EC) by binding to EC alpha v beta 3. It also promotes cell transformation to a tumorigenic phenotype, accompanied by MMP-9 and fibronectin production and cell migration. Mature human OSF-2 is 91%, 96% and 91% aa identical to rat, dog, and mouse OSF-2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Periostin/OSF-2 Antibody (AF3548)(5)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 2 applications: IHC, Western Blot.


Filter By Application
IHC
(1)
Western Blot
(2)
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Human
(2)
Rat
(1)
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Showing Publications 1 - 5 of 5.
Publications using AF3548 Applications Species
Huang, Z;Byrd, O;Tan, S;Hu, K;Knight, B;Lo, G;Taylor, L;Wu, Y;Berchuck, A;Murphy, SK; Periostin facilitates ovarian cancer recurrence by enhancing cancer stemness Scientific reports 2023-12-04 [PMID: 38049490] (IHC, Human) IHC Human
Karol Nowicki-Osuch, Lizhe Zhuang, Tik Shing Cheung, Emily L. Black, Neus Masqué-Soler, Ginny Devonshire et al. Single-Cell RNA Sequencing Unifies Developmental Programs of Esophageal and Gastric Intestinal Metaplasia Cancer Discovery 6/2/2023 [PMID: 36929873]
BA Barta, M Ruppert, KE Fröhlich, M Cosenza-Co, A Oláh, AA Sayour, K Kovács, GB Karvaly, M Biniossek, B Merkely, O Schilling, T Radovits Sex-related differences of early cardiac functional and proteomic alterations in a rat model of myocardial ischemia Journal of Translational Medicine, 2021-12-11;19(1):507. 2021-12-11 [PMID: 34895263] (Western Blot, Rat) Western Blot Rat
Cédrik Labrèche, David P. Cook, John Abou-Hamad, Julia Pascoal, Benjamin R. Pryce, Khalid N. Al-Zahrani et al. Periostin gene expression in neu-positive breast cancer cells is regulated by a FGFR signaling cross talk with TGF beta /PI3K/AKT pathways Breast Cancer Research 11/22/2021 [PMID: 34809697]
Yang L, Serada S, Fujimoto M, Terao M, Kotobuki Y, Kitaba S, Matsui S, Kudo A, Naka T, Murota H, Katayama I Periostin facilitates skin sclerosis via PI3K/Akt dependent mechanism in a mouse model of scleroderma. PLoS ONE, 2012-07-24;7(7):e41994. 2012-07-24 [PMID: 22911870] (Western Blot, Human) Western Blot Human

Review for Periostin/OSF-2 Antibody (AF3548) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF3548:
Filter by Applications
ICC
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Mohamad Youness
 ICC Human 08/01/2023
View

Summary

ApplicationImmunocytochemistry
Sample Testedprimary human cardiac fibroblasts
SpeciesHuman
LotYIGO421101

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.***

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Immunocytochemistry Periostin/OSF-2 AF3548
Enlarge
 Mohamad Youness
08/01/2023
Application: ICC
Species: Human
read this review

Bioinformatics

Gene Symbol POSTN
Uniprot