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Pentraxin 3/TSG-14 Antibody [Unconjugated]

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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, MCF-7 human breast cancer cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Pentraxin 3/TSG-14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1826) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Pentraxin 3/TSG-14 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, MCF-7 human breast cancer cell line, and ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF1826
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF1826-SP
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Pentraxin 3/TSG-14 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Pentraxin 3/TSG‑14
Glu18-Ser381
Accession # P26022
Specificity
Detects human Pentraxin 3/TSG‑14 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross‑reactivity with recombinant mouse Pentraxin 3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PTX3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF1826 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pentraxin 3/TSG-14 Antibody [Unconjugated]

  • alpha-induced protein 5
  • pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor
  • Pentaxin-related protein PTX3
  • Pentraxin 3
  • pentraxin 3, long
  • pentraxin-3
  • pentraxin-related gene, rapidly induced by IL-1 beta
  • pentraxin-related protein PTX3
  • PTX3
  • TNF alpha-induced protein 5
  • TNFAIP5
  • TSG14
  • TSG-14
  • TSG-14pentaxin-related gene, rapidly induced by IL-1 beta
  • Tumor necrosis factor alpha-induced protein 5
  • tumor necrosis factor, alpha-induced protein 5
  • Tumor necrosis factor-inducible gene 14 protein
  • tumor necrosis factor-inducible protein TSG-14

Background

Pentraxin 3 (PTX3), TSG-14, was initially identified as a TNF-alpha or IL-1 beta inducible gene (1‑3). It belongs to the pentraxin family, which was named originally for the homo-pentameric structure formed by its members (4). The pentraxin family is divided into two subfamilies: the “short” and the “long” pentraxins with approximate molecular weights of 25 kDa and 50 kDa, respectively. TSG-14 is a member of the long pentraxin subfamily, which also includes the Xenopus laevis XL-PXN1, the guinea pig apexin/p50, the rat neuronal pentraxin I (NPI) and NPR, the human neuronal pentraxin II (NPTX2) and the human neuronal activity-related pentraxin (5).

Mature secreted TSG-14 contains a pentaxin-like domain at its carboxy-terminus that shares 23‑28% amino acid (aa) sequence similarity to C-reactive protein (CRP) and serum amyloid P component (SAP), which belongs to the short pentraxin subfamily. However, the N-terminal sequence of TSG-14 does not share aa sequence homology with any of the “short” pentaxins (3). Unlike CRP and SAP, which forms pentamers only, TSG-14 forms both pentameric and higher ordered oligomers (5). Similarly to CRP and SAP, TSG-14 binds to the complement cascade component C1q (6). However, TSG-14 does not bind to phosphoethanolamine, phosphocholine, or high pyruvate agarose, which are known ligands for CRP and SAP. TSG-14 is a marker of the acute phase response and is highly expressed in advanced atherosclerotic plaques (12). While CRP and SAP are primarily produced in the liver, TSG-14 expression is strongly up‑regulated by TNF-alpha , IL-1 beta , and bacterial LPS in peripheral fibroblasts, endothelial cells, and macrophages (7). At the amino acid level, human and mouse TSG-14 share 88% aa sequence homology (8). TSG-14 concentration is elevated in the joint fluid of patients with rheumatoid arthritis (RA), indicating that TSG-14 may be a potential mediator of immune response (9). TSG‑14 may also function in the regulation of the uptake and clearance of apoptotic cells by dendritic cells (10). In vivo study showed that TSG-14 transgenic mice are more resistant to sepsis and endotoxemia compared to wild type during the inflammatory injury (11). Increased expression of TSG-14 may enhance the immune response to protect the host from infection.

  1. Lee, T.H. et al. (1990) Mol. Cell. Biol. 10:1982.
  2. Breviario, F. et al. (1992) J. Biol. Chem. 267:22190.
  3. Lee, G.W. et al. (1993) J. Immunol. 150:1804.
  4. Osmand, A.P. et al. (1977) Proc. Natl. Acad. Sci. USA 74:739.
  5. Goodman A.R. et al. (1996) Cytokine & Growth Factor Reviews 7:191.
  6. Bottazzi, B. et al. (1997) J. Biol. Chem. 272:32817.
  7. Introna, M. et al. (1996) Blood 87:1862.
  8. Altmeyer, A. et al. (1995) J. Biol. Chem. 270:25584.
  9. Luchetti, M.M. et al. (2000) Clin. Exp. Immunol. 119:196.
  10. Rovere, P. et al. (2000) Blood 96:4300.
  11. Dias, A.A.M. et al. (2001) J. Leukocyte Biol. 69:928.
  12. Rolph, M.S. et al. (2002) Arterioscler. Throm. Vasc. Biol. 22:e10-4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Pentraxin 3/TSG-14 Antibody (AF1826)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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Bioinformatics

Gene Symbol PTX3
Uniprot