PCM1 Antibody

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: FLOW, ICC/IF, IF/IHC.

Showing Publications 1 - 4 of 4.

Publications using NBP1-87195	Applications	Species
Ke Wei, Vahid Serpooshan, Cecilia Hurtado et al. Epicardial FSTL1 reconstitution regenerates the adult mammalian heart. Nature 2015-09-16 [PMID: 26375005] (ICC/IF, IF/IHC, Mouse)	ICC/IF, IF/IHC	Mouse
Nuhrenberg TG, Hammann N, Schnick T et al. Cardiac Myocyte De Novo DNA Methyltransferases 3a/3b Are Dispensable for Cardiac Function and Remodeling after Chronic Pressure Overload in Mice. PLoS One 2015-01-01 [PMID: 26098432] (IF/IHC, Mouse)	IF/IHC	Mouse
Shiratsuchi G, Takaoka K, Ashikawa T et al. RBM14 prevents assembly of centriolar protein complexes and maintains mitotic spindle integrity. EMBO J 2015-01-02 [PMID: 25385835] (ICC/IF, Human)	ICC/IF	Human
Gilsbach R, Preissl S, Gruning BA et al. Dynamic DNA methylation orchestrates cardiomyocyte development, maturation and disease. Nat Commun 2014-10-22 [PMID: 25335909] (IF/IHC, FLOW, Human)	IF/IHC, FLOW	Human

Array NBP1-87195

• PCM1 Antibodies
• PCM1 Proteins