![Western Blot: CTR9 Antibody [NB100-68205] - Detection of Human and Mouse CTR9 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CTR9 antibody NB100-68205 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CTR9-Antibody-Western-Blot-NB100-68205-img0012.jpg "Western Blot: CTR9 Antibody [NB100-68205] - Detection of Human and Mouse CTR9 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CTR9 antibody NB100-68205 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 1123 and 1173 of human Paf1/RNA polymerase II complex component homolog CTR9 using the numbering given in entry NP_055448.1 (GeneID 9646). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CTR9 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
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| Theoretical MW | 133.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB100-68205 | Applications | Species |
|---|---|---|
| Gaballa A, Gebhardt-Wolf A, Krenz B et Al. PAF1c links S-phase progression to immune evasion and MYC function in pancreatic carcinoma Nat Commun 2024-02-16 [PMID: 38365788] |
Secondary Antibodies |
Isotype Controls |
Research Areas for CTR9 Antibody (NB100-68205)Find related products by research area.
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![Immunohistochemistry: CTR9 Antibody [NB100-68205] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-CTR9 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/CTR9-Antibody-Immunohistochemistry-NB100-68205-img0010.jpg "Immunohistochemistry: CTR9 Antibody [NB100-68205] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-CTR9 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).")
![Western Blot: CTR9 Antibody [NB100-68205] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. NB100-68205 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.](http://images.novusbio.com/fullsize/CTR9-Antibody-Western-Blot-NB100-68205-img0002.jpg "Western Blot: CTR9 Antibody [NB100-68205] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. NB100-68205 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.")
![Immunohistochemistry-Paraffin: CTR9 Antibody [NB100-68205] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CTR9 used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/CTR9-Antibody-Immunohistochemistry-Paraffin-NB100-68205-img0006.jpg "Immunohistochemistry-Paraffin: CTR9 Antibody [NB100-68205] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CTR9 used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).")
![Immunohistochemistry-Paraffin: CTR9 Antibody [NB100-68205] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-CTR9 used at a dilution of 1:250.](http://images.novusbio.com/fullsize/CTR9-Antibody-Immunohistochemistry-Paraffin-NB100-68205-img0001.jpg "Immunohistochemistry-Paraffin: CTR9 Antibody [NB100-68205] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-CTR9 used at a dilution of 1:250.")
![Immunohistochemistry: CTR9 Antibody [NB100-68205] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CTR9 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/CTR9-Antibody-Immunohistochemistry-NB100-68205-img0009.jpg "Immunohistochemistry: CTR9 Antibody [NB100-68205] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CTR9 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).")
![Immunoprecipitation: CTR9 Antibody [NB100-68205] - Detection of human CTR9 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CTR9 antibody NB100-68205 (lot NB100-68205-4) used for IP at 6 ug per reaction. CTR9 was also immunoprecipitated by a previous lot of this antibody (lot NB100-68205-3). For blotting immunoprecipitated CTR9, NB100-68205 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CTR9-Antibody-Immunoprecipitation-NB100-68205-img0013.jpg "Immunoprecipitation: CTR9 Antibody [NB100-68205] - Detection of human CTR9 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CTR9 antibody NB100-68205 (lot NB100-68205-4) used for IP at 6 ug per reaction. CTR9 was also immunoprecipitated by a previous lot of this antibody (lot NB100-68205-3). For blotting immunoprecipitated CTR9, NB100-68205 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")