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Calcium-binding-protein-P22 Antibody

Images

 
Western Blot: Calcium-binding-protein-P22 Antibody [NBP1-33736] - A. 50 ug mouse liver lysate/extract 12 % SDS-PAGE
Immunocytochemistry/ Immunofluorescence: Calcium-binding-protein-P22 Antibody [NBP1-33736] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calcium binding protein P22 protein stained by Calcium ...read more
Immunohistochemistry-Paraffin: Mouse lung. Calcium binding protein P22 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: Calcium-binding-protein-P22 Antibody [NBP1-33736] - Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 12% SDS PAGE; antibody diluted at 1:1000.
Western Blot: Calcium-binding-protein-P22 Antibody [NBP1-33736] - A. 50 ug rat liver lysate/extract 12 % SDS-PAGE
Immunohistochemistry-Paraffin: SW480 xenograft, using Calcium binding protein P22 antibody at 1:100 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, RM, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Calcium-binding-protein-P22 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Calcium-binding-protein-P22. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Bovine (99%), Rhesus Macaque (100%), Chicken (96%), Xenopus (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CHP1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation
  • Western Blot 1:500-1:3000
Application Notes
IP-Assay dependent
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-33736 in the following applications:

Reactivity Notes

Zebrafish (86%), Xenopus laevis (90%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for Calcium-binding-protein-P22 Antibody

  • Calcineurin B homolog
  • Calcineurin homologous protein
  • calcium binding protein P22
  • Calcium-binding protein CHP
  • calcium-binding protein p22
  • SLC9A1 binding protein
  • SLC9A1BP

Background

This gene encodes a phosphoprotein that binds to the Na+/H+ exchanger NHE1. This protein serves as an essential cofactor which supports the physiological activity of NHE family members and may play a role in the mitogenic regulation of NHE1. The protein shares similarity with calcineurin B and calmodulin and it is also known to be an endogenous inhibitor of calcineurin activity. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Bv, Ch, RM, Xp
Applications: WB, ICC/IF, IHC, IP
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Calcium-binding-protein-P22 Antibody (NBP1-33736)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CHP1