Orthogonal Strategies: Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Analysis in human testis and liver tissues. Corresponding Pericentrin RNA-seq data are presented for the same tissues.
Independent Antibodies: Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining of human cerebral cortex, liver, placenta and testis using Anti-Pericentrin antibody NBP1-87772 (A) shows ...read more
Immunocytochemistry/ Immunofluorescence: Pericentrin Antibody [NBP1-87772] - Smear of -20C methanol-fixed mouse small intestinal epithelial cells stained for ZO-1 (red) and pericentrin (green). ICC/IF image submitted by ...read more
Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunocytochemistry/ Immunofluorescence: Pericentrin Antibody [NBP1-87772] - Staining of human cell line A-431 shows localization to centrosome. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining of human testis shows high expression.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining in human testis and liver tissues using anti-PCNT antibody. Corresponding PCNT RNA-seq data are presented for the ...read more
Immunohistochemistry-Paraffin: Pericentrin Antibody [NBP1-87772] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
This antibody was developed against Recombinant Protein corresponding to amino acids: MEDLQNQFQKELAEQRAELEKIFQDKNQAERALRNLESHHQAAIEKLREDLQSEHGRCLEDLEFKFKESEKEKQLELENLQASYEDLKAQSQEEIRRLWSQLDSARTSRQELSELHEQLLARTSRVEDLEQLKQREKTQHESELE
Marker
Centrosome Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PCNT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Mouse reactivity reported from a verified customer review.
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for Pericentrin Antibody
Kendrin
KIAA0402
MOPD2
PCN
PCNT2pericentrin B
PCNTB
pericentrin 2 (kendrin)
pericentrin
pericentrin, kendrin (KIAA0402)10KENPCTN2
pericentrin-2
pericentrin-380
pericentrin-B
SCKL4kendrin
Background
Pericentrin is encoded by this gene binds to calmodulin and is expressed in the centrosome. It is an integral component of the pericentriolar material (PCM). The protein contains a series of coiled-coil domains and a highly conserved PCM targeting motif called the PACT domain near its C-terminus. The protein interacts with the microtubule nucleation component gamma-tubulin and is likely important to normal functioning of the centrosomes, cytoskeleton, and cell-cycle progression.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Review for Pericentrin Antibody (NBP1-87772) (1)
51
Average Rating: 5 (Based on 1 review)
We have 1 review tested in 1 species: Mouse.
Reviews using NBP1-87772:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images
Ratings
Applications
Species
Date
Details
5
reviewed by: Jerrold Turner
ICC
Mouse
08/16/2021
View
Summary
Application
Immunocytochemistry
Sample Tested
Adult small intestine
Species
Mouse
Lot
A119322
Comments
Comments
We tested the antibody on smears (cytology-like preps) of mouse small intestinal epithelial cells fixed with 2% paraformaldehyde/lysine/periodate (PLP; McLean and Nakane. J Histochem Cytochem, 1974) or -20°C methanol. After permeabilizing with 0.1% saponin (PLP-fixed only) and blocking with 5% donkey serum (in PBS), slides were incubated with antibodies at the dilutions indicated below and a rat monoclonal anti-ZO-1 (tight junction protein) antibody (clone R40.76) for 2 hours at room temperature. After washing, species-specific secondary antibodies (cross-adsorbed) produced in goat were applied. ***Novus Innovators Program - new species or application used on a primary antibody.
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.