Independent Antibodies: Western Blot: PCM1 Antibody [NB100-58829] - Detection of Human PCM1 on HeLa whole cell lysate using NB100-58829. PCM1 was also immunoprecipitated by rabbit anti-PCM1 antibody NB100-58828.
The immunogen recognized by this antibody maps to a region between residue 1972 and 2022 of human pericentriolar material 1 using the numbering given in entry NP_006188.2 (GeneID 5108).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PCM1
Purity
Immunogen affinity purified
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Mouse reactivity reported in scientific literature (PMID: 30035751).
Packaging, Storage & Formulations
Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified
Alternate Names for PCM1 Antibody
hPCM-1
pericentriolar material 1 protein
pericentriolar material 1
PTC4PCM-1
Background
DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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