PCM1 Antibody

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IP, WB.

Showing Publications 1 - 3 of 3.

Publications using NB100-58828	Applications	Species
Chakravarthy B, Menard M, Brown L et al. A synthetic peptide corresponding to a region of the human pericentriolar material 1 (PCM-1) protein binds beta-amyloid (Abeta1-42 ) oligomers J Neurochem 2013-02-22 [PMID: 23432034] (WB, IP, Mouse)	WB, IP	Mouse
Moynihan KL, Pooley R, Miller PM et al. Murine CENP-F regulates centrosomal microtubule nucleation and interacts with Hook2 at the centrosome. Mol Biol Cell. 20(22):4790-803. 2009-11-01 [PMID: 19793914]
Kuriyama R, Bettencourt-Dias M, Hoffmann I et al. Gamma-tubulin-containing abnormal centrioles are induced by insufficient Plk4 in human HCT116 colorectal cancer cells. J Cell Sci 2009-06-01 [PMID: 19454482]

Array NB100-58828

• PCM1 Antibodies
• PCM1 Proteins